Log In Sign up
The largest database of trusted experimental protocols

1 kb extend marker

Manufactured by New England Biolabs
Visit Supplier

The 1 kb extend marker is a molecular weight standard used for the size determination of DNA fragments in agarose gel electrophoresis. It consists of a mixture of DNA fragments ranging in size from 1,000 to 20,000 base pairs, with distinct bands at 1,000, 2,000, 3,000, 4,000, 5,000, 6,000, 8,000, 10,000, 12,000, 15,000, and 20,000 base pairs.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop one, by Thermo Fisher Scientific (1 mentions) Qubit dsdna hs, by Thermo Fisher Scientific (1 mentions) Blood and tissue culture kit, by Qiagen (1 mentions)

2 protocols using 1 kb extend marker

1

Comparative DNA Extraction and Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
To ensure the integrity and determine the overall size of the high molecular weight genomic DNA, purified samples were loaded on a 0.5% agarose gel and compared to a 1 kb extend marker from New England Biolabs. The size of genomic DNA prepared with MNPs is significantly larger than the one prepared using spin columns (Blood and Tissue culture kit, QIAGEN; Supplementary Fig. S1). Purity and concentration of the DNA was measured using a NanoDrop One and a Qubit (dsDNA HS) from Thermo Fischer Scientific.
Erkes A., Grove R.P., Žarković M., Krautwurst S., Koebnik R., Morgan R.D., Wilson G.G., Hölzer M., Marz M., Boch J, & Grau J. (2023). Assembling highly repetitive Xanthomonas TALomes using Oxford Nanopore sequencing. BMC Genomics, 24, 151.
+ Open protocol
+ Expand
2

DNA Separation and Electroelution Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 2

Samples of DNA markers (1 kb Extend marker, New England Biolabs) was loaded into sample well of two lanes of a SageHLS cassette. The DNA was separated and electroeluted in using the following electrophoresis conditions: 0.75% agarose, 50 mM Tris, 29 mM TAPS, 0.1 mM EDTA, pH 8.7, 55 V continuous field (DC), 50 minutes, gel temperature 30° C. Electroeluted fractions from all elution wells were analyzed on an analytical agarose slab gel (FIGS. 11A-11B). Evidence of electrophoretic mobility compression in the HLS separation run is seen in Fraction #2 (that is, fragments 10-48.5 kb comigrate and are found together in fraction #2, and no DNA is found in Fraction #1). Therefore, due to the compression phenomenon, under these conditions, all DNA greater than 10 kb will be found in fraction #2. Fractions #5 and #6 contain fragments ranging from 1-2 kb.

US11867661B2. Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification (2024-01-09). Sage Science, Inc. [US]. Inventors: T. Christian Boles [US].
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!