Total RNA for each sample was extracted pooled 10 BPs using the RNeasy Plus Micro kit (catalog # 74004, QIAGEN, Venlo, Netherlands) according to the manufacturer’s protocol. DNase I treatment was performed using spin columns. RNA was reverse-transcribed using the SuperScript III First-Strand Synthesis System. Quantitative real-time polymerase chain reaction (qPCR) was performed using a StepOnePlus Real-Time PCR system. cDNA was amplified using the Power SYBR Green PCR Master Mix or TaqMan™ Fast Advanced Master Mix. The experiment was performed in triplicate. Target gene expression was normalized to hypoxanthine phosphoribosyltransferase 1 (Hprt1).112 (link) cDNA from the brain tissue of post-hatched 1-day-old chickens was used to generate standard curves for each gene. Relative quantification was performed using Real-Time PCR System Software v2.0, with the 2−ΔΔCT method (Thermo Fisher Scientific). The primers used are shown in the key resources table .
Real time pcr system software v2
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Real-Time PCR System Software v2.0 is a software application designed to operate and control Thermo Fisher Scientific's real-time PCR systems. The software provides the necessary functionality to set up, run, and analyze real-time PCR experiments.
Automatically generated - may contain errors
Lab products found in correlation
2δδct method, by Thermo Fisher Scientific (2 mentions)
Rneasy plus micro kit, by Qiagen (2 mentions)
Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions)
Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (1 mentions)
2 protocols using real time pcr system software v2
1
Real-Time qPCR of Chicken Brain
2
Quantifying Gene Expression in Chicken Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA for each sample was extracted pooled 10 BPs using the RNeasy Plus Micro kit (catalog # 74004, QIAGEN, Venlo, Netherlands) according to the manufacturer’s protocol. DNase I treatment was performed using spin columns. RNA was reverse-transcribed using the SuperScript III First-Strand Synthesis System (catalog #N8080234, Thermo Fisher Scientific, Waltham, MA, USA). Quantitative real-time polymerase chain reaction (qPCR) was performed using a StepOnePlus Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). cDNA was amplified using the Power SYBR Green PCR Master Mix (catalog #4367659, Thermo Fisher Scientific, Waltham, MA, USA). Target gene expression was normalized to hypoxanthine phosphoribosyltransferase 1 (Hprt1, Hassanpour et al., 2019 (link)). cDNA from the brain tissue of post-hatched 1-day-old chickens was used to generate standard curves for each gene. Relative quantification was performed using Real-Time PCR System Software v2.0, with the 2−ΔΔCT method (Thermo Fisher Scientific, Waltham, MA, USA). The following primers were used:
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!