Eclipse ti u digital camera

Harvested calvaria specimens were dehydrated and subsequently infiltrated with resin (Technovit, Heraeus Kulzer GmbH, Germany) for 23 days. The method for the slide preparation was described previously [20 (link)]. The polished sections were viewed using a Nikon fluorescence microscope apparatus with bright field, TRITC and FITC filters, and a Nikon Eclipse Ti-U digital camera (Nikon Instruments Inc., Melville, NY, USA). Each fluorescent image was merged to measure the distance between fluorescent labeled layers at the interface between native bone and the implanted scaffold (INBS) and at the central pore area with the Nikon NIS Elements software (Nikon Instruments Inc., Melville, NY, USA) tools. A total of five measurements were made along the span of each double label. The MAR was calculated using the following equation: MAR (μm/day) = ∑_χ/nt, where ∑_χ is the sum of all the measurements between double labels, n is the total number of measurements, and t is the time (days) interval expressed [25 –27 (link)]. The mean distance of the five regions was subject to statistical analysis and the overall mean distance was calculated for each group. Two-way ANOVA was used to compare the means among four groups.

The explanted calvaria was dehydrated and subsequently infiltrated with resin (Technovit, Heraeus Kulzer GmbH, Hanau, Germany) for 32 days. The gross tissue was adhered to a mold with Technovit 7230 and embedded with Technovit 7200 embedding media. Air bubbles in the mold were eliminated in a vacuum chamber. The sample was polymerized for 2 to 6 h with yellow and blue lamps. The slides were mounted to both sides of the sample, and the sample was cut to the area of interest (EXAKT Model 310 sectioning saw, Norderstedt, Germany). The desired sections were ground until their thickness reached between 65 and 70 mm (EXAKT Model 400 microgrinding system, Norderstedt, Germany). Once polished, sections were stained using Stevenel’s blue solution and counterstained with Van Gieson’s stain. Color images of the stained sections were acquired with a Nikon inverted microscope apparatus with a Nikon Eclipse Ti-U digital camera (Nikon Instruments Inc., Melville, NY, USA).