All of the NPC cell lines including CNE-1, CNE-2, HNE-1, SUNE-1, and the normal epithelial cell line NP69 were from the key laboratory of the Sun Yat-Sen University cancer center (Guangzhou, People’s Republic of China). All NPC cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). NP69 cells were maintained in serum-free medium (STEMCELL Technologies, Vancouver, BC, Canada). These cells were used within passage number four to six. All cells were cultured at 37°C in a 5% CO2-humidified incubator.
Antibodies used in this study included anti-Met antibodies for immunohistochemistry (Ventana, Harvard, MA, USA) and Western blot (Santa Cruz Biotechnology Inc., Dallas, TX, USA). Anti-phospho-Met (Upstate, NY, USA), anti-HGF, anti-AKT, anti-phospho-AKT, anti-MAPK, anti-ERK, anti-phospho-ERK, anti-vimentin, anti-Snail, and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for Western blot were purchased from the same company (CST, Danvers, MA, USA), while Anit-phospho-Met were purchased from another company (Upstate, Syracuse, NY, USA). PF-2341066 was dissolved in dimethyl sulfoxide, allotted, and stored at −20°C for use in vitro while dissolved in water for intragastric administration.
Antibodies used in this study included anti-Met antibodies for immunohistochemistry (Ventana, Harvard, MA, USA) and Western blot (Santa Cruz Biotechnology Inc., Dallas, TX, USA). Anti-phospho-Met (Upstate, NY, USA), anti-HGF, anti-AKT, anti-phospho-AKT, anti-MAPK, anti-ERK, anti-phospho-ERK, anti-vimentin, anti-Snail, and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for Western blot were purchased from the same company (CST, Danvers, MA, USA), while Anit-phospho-Met were purchased from another company (Upstate, Syracuse, NY, USA). PF-2341066 was dissolved in dimethyl sulfoxide, allotted, and stored at −20°C for use in vitro while dissolved in water for intragastric administration.