Mission shrna containing lentiviral particles

Manufactured by Merck Group

Mission shRNA containing lentiviral particles is a laboratory tool used to deliver short hairpin RNA (shRNA) sequences into target cells. The lentiviral particles provide a means to introduce the shRNA genetic material into cells, enabling the study of gene function through RNA interference.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Celltox green cytotoxicity assay, by Promega (1 mentions) Cisplatin, by Merck Group (1 mentions) Snapwell membranes, by Corning (1 mentions) Rat tail collagen 1, by BD (1 mentions)

Close spelling variants of this product

MISSION shRNA lentiviral particles MISSION® lentiviral particles ShRNA lentiviral particles MISSION shRNA Lentiviral particles Lentiviral shRNAs

2 protocols using mission shrna containing lentiviral particles

Investigating HEI-OC1 Hair Cell Line

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The cochlear hair cell line HEI-OC1 [23 (link)] was a generous gift of Dr. Kalinec (House Ear Institute, Los Angeles, CA, USA). High-glucose Dulbecco's Modified Essential Medium (DMEM, cat no. 11965–092) and fetal bovine serum (cat no. 26140–111) were purchased from Life Technologies. Cisplatin (cat no. 479306) and Mission shRNA containing lentiviral particles were purchased from Sigma-Aldrich. ATPlite one step assay (cat no. 6016731) was purchased from Perkin Elmer and CellTox Green Cytotoxicity Assay (cat no. G8741) from Promega.

Grondin Y., Bortoni M.E., Sepulveda R., Ghelfi E., Bartos A., Cotanche D., Clifford R.E, & Rogers R.A. (2015). Genetic Polymorphisms Associated with Hearing Threshold Shift in Subjects during First Encounter with Occupational Impulse Noise. PLoS ONE, 10(6), e0130827.

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Stable Cell Line Generation for Knockdown Studies

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CFBE41o-, wt-HBE41o- or primary bronchial epithelial cells were infected with Mission shRNA containing lentiviral particles (Sigma-Aldrich) at MOI 3 for 16 hours before seeding onto 12 mm Snapwell membranes (Corning, Cambridge, MA) coated with rat tail collagen I (BD Biosciences). Lentivirus infection is covered under TSRI approval #01–13-10–07 and all steps were carried out in a BSL2/3 certified laboratory. Stable cell lines were created by puromycin selection (20 μg/ml) and serial dilution after infection. Quantification of CK2 knockdown was carried out with ImageJ (NIH, http://imagej.nih.gov/ij) (68 (link)).

Pankow S., Bamberger C, & Yates JR I.I.I. (2019). A post-translational modification code for CFTR maturation is altered in cystic fibrosis. Science signaling, 12(562), eaan7984.

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