Apgltqalntk

Deionized water was produced by a Millipore Advantage A10 system (Millipore, Bedford, MA, USA). Chloral hydrate was ordered from KeLong Chemical (Chengdu, China), 90% ethanol (HPLC grade) from Thermo Fisher Scientific (Rockford, IL, USA), sequencing-grade trypsin from Promega (Madison, WI, USA), ammonium formate from Thermo Fisher Scientific (Rockford, IL, USA), and HPLC-grade acetonitrile (CH₃CN) from Thermo Fisher Scientific (Rockford, IL, USA). Isotopically-labeled peptide with 99.8% purity: APGLTQALNTK (13C6, 15N2), and unlabeled peptide with the same sequence with 99.0% purity: APGLTQALNTK, were synthesized by GenScript (Nanjing, China).

Chloral hydrate was purchased from KeLong Chemical (Chengdu, China). Deionized water was purified by a Millipore Advantage A10 system (Millipore, Bedford, MA, USA). An amount of 90% ethanol (HPLC grade) was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Sequencing grade trypsin was obtained from Promega (Madison, WI, USA). Ammonium formate was purchased from Thermo Fisher Scientific (Rockford, IL, USA). HPLC grade acetonitrile (CH3CN) was obtained from Thermo Fisher Scientific (Rockford, IL, USA). Isotopically labeled peptide with 99.8% purity: APGLTQALNTK (13C6, 15N2), and unlabeled peptide with the same sequence with 99.0% purity: APGLTQALNTK, were purchased from GenScript (Nanjing, China).