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Xl30 environmental sem feg

Manufactured by Thermo Fisher Scientific
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The XL30 Environmental SEM-FEG is a scanning electron microscope that utilizes a field emission gun (FEG) for high-resolution imaging of samples in an environmental chamber. It is designed to observe samples in their natural state without the need for extensive sample preparation.

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Lab products found in correlation

Vacuum desk 2 sputter coater, by Denton (1 mentions) Vacuum sputter coater, by Denton (1 mentions)

Close spelling variants of this product

XL30 FEG-SEM XL30 environmental XL30 FEG XL30 SEM FEG-SEM

2 protocols using xl30 environmental sem feg

1

Dragline Silk Sample Preparation and SEM Imaging

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The dry dragline silk sample was taped on the sample holder and coated with Au/Pd in a Denton vacuum sputter coater desk II for 3 min under a pressure of 200 mTorr. The deposition rate was 5 nm/min with a current of 20 mA. The SEM image was taken using a FEI XL30 Environmental SEM-FEG. The SEM was operating at a vacuum pressure less than 9 × 10−5 mbar and a beam current of 7.00 kV.
Xu D., Shi X., Thompson F., Weber W.S., Mou Q, & Yarger J.L. (2015). Protein secondary structure of Green Lynx spider dragline silk investigated by solid-state NMR and X-ray diffraction. International journal of biological macromolecules, 81, 171-179.
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2

Characterizing Webspinner Silk Fibers

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Cleaned webspinner silks were secured to conductive carbon tape, then were gold coated using a Denton vacuum sputter coater for 3 minutes at a deposition rate of 5 nm min−1. The SEM was performed using a XL30 Environmental SEM-FEG built by FEI. The secondary electron (SE) detector was used for imaging. Images were collected under a vacuum pressure of less than 9 × 10−5 mbar and with a beam current of 5 kV. SEM images were processed using Gwyddion version 2.31 to measure fiber diameters from multiple images.37
Addison J.B., Popp T.M., Weber W.S., Edgerly J.S., Holland G.P, & Yarger J.L. (2014). Structural characterization of nanofiber silk produced by embiopterans (webspinners). RSC advances, 4(78), 41301-41313.
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