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Collagenase

Manufactured by HiMedia
Sourced in India

Collagenase is a laboratory enzyme used for the dissociation and isolation of cells from tissues. It functions by breaking down collagen, a primary structural component of the extracellular matrix.

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2 protocols using collagenase

1

Fibroblast Morphology and Arecoline/Collagenase Effects

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The third passage was taken into consideration for the morphological assessment of the fibroblasts and the effects of arecoline (Sigma-Aldrich) and collagenase (HiMedia) on a concentration in a dose-dependent manner.
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2

Cytotoxicity Evaluation of Common Cell Culture Reagents

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Dulbecco's Modified Eagle's medium (DMEM), HAM's F12 K, α-MEM, trypsin, glutamine, fetal bovine serum (FBS), MSC qualified FBS were obtained from GIBCO by Life Technologies. 3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), carbon tetra chloride (CCl4) were purchased from Sigma – Aldrich Company. Collagenase, Penicillin and Streptomycin, and L-15 Medium were purchased from Hi Media Laboratories, India.
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