Rabbit anti-α-SMA (Cat No. ab179467), Col4α1 (Cat No. ab6586), CD206 (Cat No. ab64693), CD68 (Cat No. ab125212), PPARα (Cat No. ab227074), and mouse anti-CD86 (Cat No. ab220188) antibodies were purchased from Abcam (Cambridge, MA). Rabbit anti-F4/80 (Cat No. #30325), NF-κB-p65 (Cat No. #8242), and phosphorylation of NF-κB-p65 (Cat No. #3033) antibodies were purchased from Cell Signaling Technology. Rabbit anti-β−actin (Cat No. AC028) was purchased from ABclonal (Wuhan, China). Mouse AST ELISA kit (Cat No. ab263882), ALT assay kit (Cat No. ab241035), ALP assay Kit (Cat No. ab267583), gamma-glutamyl transferase (γ-GT) assay kit (Cat No. ab241029), triglyceride assay kit (Cat No. ab65336), free fatty acid assay (Cat No. ab65341), ROS detection assay (Cat No. ab139476), caspase-3 assay kit (Cat No. ab39401), and mitochondrial complex I enzyme activity microplate assay kit (Cat No. ab109721) and mitochondrial complex III activity assay kit (Cat No. ab287844) were obtained from Abcam (Cambridge, MA). Glycyrrhetinic acid (Cat No. HY-N0375), betaine (Cat No. HY-B0710), ursolic acid (Cat No. HY-N0140), and wogonin (Cat No. HY-N0400) were purchased from MedChemExpress (Shanghai, China).
Wogonin
Manufactured by MedChemExpress
Sourced in China, United States
Wogonin is a flavonoid compound isolated from the root of the Scutellaria baicalensis plant. It is a chemical reagent commonly used in research applications, particularly in the field of medicinal chemistry and biochemistry. Wogonin is known for its potential anti-inflammatory, antioxidant, and neuroprotective properties, making it a subject of ongoing scientific investigation. The product is available in a pure powder form for laboratory use.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharides (lps), by Merck Group (2 mentions)
Alp assay kit, by Abcam (1 mentions)
Caspase 3 assay kit, by Abcam (1 mentions)
Triglyceride assay kit, by Abcam (1 mentions)
Alt assay kit, by Abcam (1 mentions)
Rabbit anti f4 80, by Cell Signaling Technology (1 mentions)
Mouse ast elisa kit, by Abcam (1 mentions)
Mitochondrial complex 3 activity assay kit, by Abcam (1 mentions)
Ros detection assay, by Abcam (1 mentions)
Col4α1, by Abcam (1 mentions)
Mouse anti cd86, by Abcam (1 mentions)
Free fatty acid assay, by Abcam (1 mentions)
Rabbit anti α sma, by Abcam (1 mentions)
Cd206, by Abcam (1 mentions)
Rabbit anti β actin, by ABclonal (1 mentions)
Nf κb p65, by Cell Signaling Technology (1 mentions)
Pparα, by Abcam (1 mentions)
Cd44 fitc, by BioLegend (1 mentions)
Cd34 pe, by BioLegend (1 mentions)
Cd45 af700, by BioLegend (1 mentions)
6 protocols using wogonin
1
Hepatoprotective Agents and Cellular Mechanisms
2
Reactive Oxygen Species Detection Assay
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Reactive Oxygen Species Detection Assay Kit was purchased from BioVision (San Francisco, CA, USA). Wogonin was purchased from MedChemExpress (Israel Shekel), and LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA). The following antibodies were used for cell surface staining: CD34-PE, CD44-FITC, CD29-PE-Cy7, Sca-1-AF700, CD45-AF700, and CD49e-APC are all from BioLegend and Mouse IL-10 ELISA Set and Zombie Green™ Fixable Viability Kit were purchased from BD Biosciences. Mouse IL-10 MAb was purchased from R&D Systems.
3
Cytotoxicity Evaluation of Compounds Against HBV-Replicating Cell Lines
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Two HBV-replicating cell lines HepG2.2.15 and HepG2.A64 were employed in the study. HepG2.A64 as an ETV-resistant HBV-replicating cell line has been employed previously (Liu et al., 2016 (link); Liu et al., 2018 (link)). Compared to HepG2.2.15 cells, HepG2.A64 cells generated comparable HBV DNA, higher HBsAg but lower HBeAg. The cytotoxicity of LWWL (Shibo Jindu, Zibo, China) and four compounds quercetin, luteolin, wogonin, and kaempferol (purchased from MedChemExpress Co., Ltd., Monmouth Junction, United States) on cells were analyzed using Cell Counting Kit-8 (Dojindo Laborarories, kyushu, Japan) according to the manufacturer’s instructions. The median cytotoxic concentration (CC50) was calculated. The molecular and cellular studies were carried out in Biosafety level-2 (BSL-2) laboratory at Center Laboratory, The Fifth Medical Center of Chinese PLA General Hospital. All manipulations were strictly conducted according to the instructions of the laboratories.
4
Cytotoxicity assessment of baicalein and wogonin
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Baicalein (purity ≧ 98%) and wogonin (purity ≧ 98%) were purchased from MedChemExpress (New Jersey, USA). The cytotoxicity control test of the drug was detected using Cell Counting Kit-8 (CCK-8) (Beyotime, Hangzhou, China). HT22 cells were seeded in a 96-well plate at a density of 3 × 105, and after culturing for 24 h, different concentrations of Baicalein (0, 10, 20, 40, 80, 160, 320 µM) and wogonin (0, 5, 10, 20, 40, 80, 160 µM) were added respectively. After 24 h of incubation, cell viability was detected with CCK8, and the OD value (450 nm) of each well was measured with a SpectraMAX M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA), which was used to screen out the safe concentration range of the drug.
5
Investigating Endothelial Cell Responses
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Wogonin, bosentan, and PDGF-BB were purchased from MedChemExpress (Israel Shekel). High-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Thermo Fisher Scientific Inc., Waltham, MA, USA). Phalloidin and Hochest 33342 were purchased from Abcam (Cambridge, UK). Primary antibodies specific for vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), and HIF-1α were procured from ProteinTech (Chicago, IL, USA). Primary antibodies against NOX4, α-smooth muscle actin (α-SMA), and calponin 1 were purchased from Abcam (Cambridge, UK). The Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Laboratories (Kumamoto, Japan). Other reagents and chemicals were all analytical grade.
6
Wogonin Modulates Murine B Cell Functions
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The spleen was minced and passed through a 70 μm nylon cell strainer; lymphocytes were isolated via Ficoll-Hypaque (Tianjin HaoYang Biological Manufacture, Tianjin, China). Cells were resuspended in completed RPMI-1640 medium (Gibco, Grand Island, USA) containing with 10% heat-inactivated fetal calf serum (Hyclone, Australia), 100 μg/mL streptomycin, 100 U/mL penicillin, 2 mM L-glutamine, and 50 μM 2-mercaptoethanol (Life Technologies). CD19+ cells were purified by negative selection nanobeads according to the manufacturer's protocols (BioLegend, San Diego, CA, USA). The purity of CD19+ cells was verified on FACS Arial II (BD Biosciences). 1 × 106/mL purified B cells were cultured with/without 2 μg/mL LPS (Sigma-Aldrich, St Louis, MO, USA) for 6 h, 12 h, 24 h, and 48 h in the presence of Wogonin (MedChemExpress, China) with different concentrations (0, 12.5, 25, and 50 μM).
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