Mounting media

Manufactured by Thermo Fisher Scientific

Sourced in United States

Mounting media is a laboratory product used to prepare microscope slides for analysis. It serves the core function of securing and preserving samples on the slide, allowing for clear visualization and examination under a microscope.

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Lab products found in correlation

Lsm 710, by Zeiss (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Normal goat serum (ngs), by Thermo Fisher Scientific (2 mentions) Rhodamine red anti mouse and cyanine 5 anti rabbit secondary antibodies, by Thermo Fisher Scientific (2 mentions) Fv1000 confocal microscope, by Olympus (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Collagen type 2, by BD (2 mentions) Antigen unmasking solution citrate buffer ph 6, by Vector Laboratories (2 mentions) Rotatory microtome, by Leica (1 mentions) Harris hematoxylin solution, by Merck Group (1 mentions) Eosin y solution, by Merck Group (1 mentions) Microscopy slides, by Thermo Fisher Scientific (1 mentions) Draq5, by Cell Signaling Technology (1 mentions) Tcs sp5 confocal microscope, by Leica (1 mentions) A1 confocal laser microscope system, by Nikon (1 mentions) Nunc lab tek 2 chamber slide, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by AAT Bioquest (1 mentions) Triton x 100, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Axioscope fluorescence microscope, by Zeiss (1 mentions)

Spelling variants of this product

ProLong Diamond antifade mounting media (41 citations) Prolong Gold mounting media with DAPI (25 citations) Cytoseal XYL mounting media (14 citations) ProLong mounting media (9 citations) Permanent mounting media (4 citations) SlowFade Gold with DAPI mounting media (3 citations) Pro–long Gold anti-fade DAPI mounting media (2 citations) SlowFade Diamond Antifade with DAPI mounting media (2 citations) PermaFluor Aqueous Mounting Media (2 citations) SlowFade® gold antifade mounting media with 4′,6-Diamidin-2-phenylindol (DAPI) (2 citations)

34 protocols using mounting media

Histopathological Analysis of Brain Tissue

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Histopathological study was performed as a previously described routine method [12 (link)]. Briefly, brain tissues were fixed in 4% neutral buffered
paraformaldehyde and washed with tap water for overnight. They were dehydrated in a graded series of ethyl alcohol (70 to 100%), cleaned with xylene, and embedded in paraffin with routine
protocol. Paraffin blocks were sectioned at a thickness of 4 µm using a rotatory microtome (Leica, Wetzlar, Germany). Sections were mounted on slide glass and dried on slide
warmer. They were immersed in xylene, rehydrated in a graded series of ethyl alcohol (100 to 70%), and washed with water. Sections were stained with Harris hematoxylin solution (Sigma
Aldrich) and eosin Y solution (Sigma Aldrich). After staining, sections were dehydrated with a graded ethyl alcohol series, cleaned in xylene, and subsequently mounted with mounting media
(Thermo Fisher Scientific, Waltham, MA, U.S.A.). Mounted sections were observed with Olympus microscope (Olympus, Tokyo, Japan).

SHAH M.A., PARK D.J., KANG J.B., KIM M.O, & KOH P.O. (2019). Baicalin attenuates lipopolysaccharide-induced neuroinflammation in cerebral cortex of mice via inhibiting nuclear factor kappa B (NF-κB) activation. The Journal of Veterinary Medical Science, 81(9), 1359-1367.

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Granulation Tissue Formation Analysis

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Mice (n=5 mice/group) were sacrificed and skin tissues were harvest at day 6 post-wounding. Cross-sections (5 µm) of paraffin-embedded skin tissues were stained with hematoxylin for 5 min and eosin for 2 min (H&E). Stained tissues were mounted with mounting media (Thermo Fisher scientific, Inc.) and observed under light microscopy (x100 magnification). The granulation tissue was identified by the presence of tissue matrix, immune cells, vascular tissue and fibroblasts, and the formation of granulation tissue area was defined as the area between underneath the neo-epithelium and above the subcutaneous fat tissue. The relative granulation tissue area ratios were calculated on the digital images using ImageJ based on the wound closure area, area of dermis and epidermis.

Lee B.C., Song J., Lee A., Cho D, & Kim T.S. (2020). Erythroid differentiation regulator 1 promotes wound healing by inducing the production of C-C motif chemokine ligand 2 via the activation of MAP kinases in vitro and in vivo. International Journal of Molecular Medicine, 46(6), 2185-2193.

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Visualizing MSC-Antibody Interactions

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MSCs stably expressing green fluorescent protein were seeded on pre-sterilized 100 mm coverslips in a 6-well plate (50,000 cells/well). One day later, MSCs were incubated with PA-PG (50 µg/mL) in serum-free media for 1 h at 37 °C. The cells were then washed twice with DPBS and then incubated with AF647-labeled Ab (100 µg/mL) for 1 h at 4 °C. The cells were then washed, labeled with Hoechst 33342 dye (to stain the nuclei), and fixed using 4% paraformaldehyde for 15 min at room temperature. The cells were then washed, and the coverslips were mounted on microscopy slides using mounting media (Thermo Fisher Scientific). The slides were then imaged for GFP (MSC), Hoechst (nuclei), and AF647 (Ab) fluorescence using a confocal microscope (Leica, Wetzlar, Germany).

Nethi S.K., Li X., Bhatnagar S, & Prabha S. (2023). Enhancing Anticancer Efficacy of Chemotherapeutics Using Targeting Ligand-Functionalized Synthetic Antigen Receptor-Mesenchymal Stem Cells. Pharmaceutics, 15(6), 1742.

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Rhodamine B Cellular Localization Assay

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SKOV3 cells were seeded in a four-well chamber slide at a density of 1 × 10⁴ cells in a total volume of 400 μl for each well and allowed to incubate overnight. Media was replaced with formulations loaded with Rhodamine B and incubate for 6 h. Following, the supernatant was, and cells were washed thrice with 400 μl of PBS. Then, cells were fixed with 3% paraformaldehyde solution in PBS for 10 min at room temperature. This solution was then discarded, and cells were washed thrice with 400 μl of PBS. The nucleus was stained with a cell permeable far-red fluorescent DNA dye DRAQ5^® (Cell Signaling Technology, USA) at a concentration of 5 μM for 10 min at room temperature. Cells were then washed thrice with 400 μl of PBS. The chambers were then removed, and 1 drop of mounting media (Thermo Fisher Scientific) was added per coverslip. The coverslips were mounted on the slide and let sit for 1 h in the dark. Images were recorded using Leica TCS SP5 confocal microscope.

Luong D., Kesharwani P., Alsaab H.O., Sau S., Padhye S., Sarkar F.H, & Iyer A.K. (2017). Folic acid conjugated polymeric micelles loaded with a curcumin difluorinated analog for targeting cervical and ovarian cancers. Colloids and surfaces. B, Biointerfaces, 157, 490-502.

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Immunofluorescent Characterization of TDP-43

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SH-SY5Y cells, cultured on poly-L-lysine treated glass coverslip, were transfected at 60%–80% confluency with siRNAs for 48 h. Cells were fixed in 4% paraformaldehyde, permeabilized in 0.25% Triton-X, blocked in blocking buffer (3% BSA, 5% Normal Goat Serum (Thermo), 0.1% Tween) and incubated in primary antibody (TDP-43: 1:400; J2: 1:100). Slides were incubated with Rhodamine red anti-mouse and Cyanine 5 anti-rabbit secondary antibodies (Thermo Scientific; diluted 1:750) and were mounted in mounting media (Thermo). Cells were imaged with an Olympus FV1000 confocal microscope.

Dunker W., Ye X., Zhao Y., Liu L., Richardson A, & Karijolich J. (2021). TDP-43 prevents endogenous RNAs from triggering a lethal RIG-I-dependent interferon response. Cell reports, 35(2), 108976.

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Immunofluorescence Staining of Primary Hippocampal Neurons

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Primary hippocampal neurons, grown on glass coverslips coated with poly-L-lysine, were fixed in 4% paraformaldehyde (PFA)–phosphate buffered saline (PBS) for 15 min and then permeabilized by 0.1% Triton X-100–PBS for 10 min. After incubation with 10% normal goat serum (NGS) blocking buffer for 1 h at room temperature, the neurons were incubated with primary antibody at 4 °C overnight. After washing with PBS, the neurons on the coverslip were incubated with secondary antibodies for 1 h at room temperature. The neurons were washed with PBS 3 times for 5 min and then were mounted on positively charged glass slides with mounting media (Thermo Scientific, Kalamazoo, MI, USA, 9990402). The neurons were imaged using a Nikon A1 confocal laser microscope system.

Lee S.E, & Lee G.H. (2021). Reelin Affects Signaling Pathways of a Group of Inhibitory Neurons and the Development of Inhibitory Synapses in Primary Neurons. International Journal of Molecular Sciences, 22(14), 7510.

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Immunohistochemical Profiling of Zinc Transporters in HCC

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To estimate the correlations of zinc transporters and HCC prognosis immunohistochemical staining was performed in liver resection samples. Previously characterized HCC and cirrhotic FFPE tissue blocks were cut at 4 µm and dried for 1 h at 60 °C. Immunohistochemistry (IHC) staining was applied to tissue sections using fully automated Bond-Max IHC system (Leica). Subsequently, the slides were stained with antibodies against ZnT1 (MBS3013756), ZnT7 (MBS7605982), ZIP7 (MBS8525931), ZIP5 (MBS9412273) and ZIP14 (MBS9404998). All primary antibodies were purchased from MyBiosource. The sections were also counterstained with Mayer’s hematoxylin&eosin and cover slipped with mounting media (Thermo).

, & Bitirim C.V. (2021). The role of zinc transporter proteins as predictive and prognostic biomarkers of hepatocellular cancer. PeerJ, 9, e12314.

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Macrophage-Lactobacillus Interaction Assay

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J774A.1 macrophages were seeded on top of a microscope slide inside 60 mm petri dishes at a density of 0.8 × 10⁶ cells overnight. Upon adherence, cells were exposed to Lpb. plantarum strain C904, LT52, or IMC513 at a ratio of 10:1 (bacteria/macrophages) for 6 h. In brief, single colonies of each strain were picked, diluted in PBS and used to cover J774A.1 cells on individual microscope slides. Both cell and bacterial staining was performed using Shandon^TM Kwik-Diff^TM Giemsa stains (ThermoFisher, Waltham, MA, United States). Briefly, the microscope slides were dipped orderly 15 times in methanol, eosin and finally methylene Blue, washed with tap water. Samples were let to dry, mounted with mounting media (Thermo Fisher, Waltham, MA, United States) and hold using a coverslide. A light field microscope (Zeiss, Germany) was used to take pictures at 100× magnification.

Garcia-Gonzalez N., Nuñez-Sanchez M.A., Villoria Recio M., Battista N., Gahan C.G, & Corsetti A. (2021). Immunomodulation of J774A.1 Murine Macrophages by Lactiplantibacillus plantarum Strains Isolated From the Human Gastrointestinal Tract and Fermented Foods. Frontiers in Microbiology, 11, 557143.

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Immunofluorescence Assay for RAD51 in MDA-MB-231 Cells

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MDA-MB-231 cells grown on 8-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific, Inc.) were fixed with 4% paraformaldehyde (Sigma-Aldrich; Merck KGaA) for 30 min at room temperature, followed by incubation of the cells with a permeabilization/blocking buffer containing 0.5% Triton-X 100 (Sigma-Aldrich; Merck KGaA) and 1% bovine serum albumin (Sigma-Aldrich; Merck KGaA) in PBS for 1 h at room temperature. The cells were then sequentially incubated with rabbit polyclonal antibodies against human RAD51 (cat. no. 100469; 1:200 dilution; GeneTex) overnight at 4°C and Alexa Fluor 555-conjugated donkey polyclonal antibodies against rabbit IgG (cat. no. A32794; 1:500 dilution; Thermo Fisher Scientific, Inc.) for 1 h at room temperature, followed by co-staining with 2 µg/ml FITC-conjugated phalloidin (for F-actin) (cat. no. A12379; Thermo Fisher Scientific, Inc.) and 1 µg/ml DAPI (AAT Bioquest) (for the cell nuclei) for 20 min at room temperature. After the cells were mounted onto coverslips with Mounting Media (Thermo Fisher Scientific, Inc.), images of the cells were captured by Zeiss Axioscope fluorescence microscope (Carl Zeiss GmbH) and analyzed by ImageJ (NIH).

Wang Y.Y., Cheng K.H., Hung A.C., Lo S., Chen P.Y., Wu Y.C., Hou M.F, & Yuan S.S. (2023). Differential impact of cytoplasmic vs. nuclear RAD51 expression on breast cancer progression and patient prognosis. International Journal of Oncology, 64(2), 12.

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Immunofluorescent Characterization of TDP-43

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Dunker W., Ye X., Zhao Y., Liu L., Richardson A, & Karijolich J. (2021). TDP-43 prevents endogenous RNAs from triggering a lethal RIG-I-dependent interferon response. Cell reports, 35(2), 108976.

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