Equine apoferritin

Manufactured by Merck Group

Sourced in Germany

Equine Apoferritin is a lab equipment product from Merck Group. It is a protein complex that can be used as a standard or control in various laboratory applications.

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Lab products found in correlation

Bovine thyroglobulin, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Em gp2, by Leica (1 mentions)

Spelling variants of this product

Apoferritin (58 citations) Apoferritin from equine spleen (3 citations)

2 protocols using equine apoferritin

SARS-CoV-2 RBD Protein Purification and SAXS

Cited 1 time

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Samples were prepared according to standardized approaches for synchrotron SAXS data collection^{44 (link)}.
The RBD from Spike glycoprotein, SARS-CoV-2, was expressed in HEK293-F cells and purified as previously described^{37 (link)}. Purified RBD was stored frozen (−80 °C) and dialyzed overnight into a PBS buffer before measurement.
Standard proteins were purchased from Sigma (Darmstadt, Germany): Bovine Thyroglobulin (Sigma-T1001), Equine Apoferritin (Sigma-A3660), and Bovine Serum Albumin (Sigma-A7030). Each standard was dissolved in a PBS buffer and filtered using an Ultrafree®-MC spin-filter device (12,000×g, 4 min.) prior to measurement.

Blanchet C.E., Round A., Mertens H.D., Ayyer K., Graewert M., Awel S., Franke D., Dörner K., Bajt S., Bean R., Custódio T.F., de Wijn R., Juncheng E., Henkel A., Gruzinov A., Jeffries C.M., Kim Y., Kirkwood H., Kloos M., Knoška J., Koliyadu J., Letrun R., Löw C., Makroczyova J., Mall A., Meijers R., Pena Murillo G.E., Oberthür D., Round E., Seuring C., Sikorski M., Vagovic P., Valerio J., Wollweber T., Zhuang Y., Schulz J., Haas H., Chapman H.N., Mancuso A.P, & Svergun D. (2023). Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Communications Biology, 6, 1057.

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Cryo-EM Single Particle and Tomography

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Equine Apoferritin at 7 mg/ml (Sigma, A3641) was prepared on 1.2/1.3 gold Ultrafoil grids, glow discharged in a PELCO easiGlow system for 2 min with a 15 mA current, for single particle reconstructions and on 1.2/1.3 carbon Quantifoil grids, glow discharged for 30 s with a 15 mA current, for tomography using a Leica EM GP2 automated plunge freezer. A 4 μL volume was applied to the grids which were back-side blotted using the ‘auto-blot’ function of the EM GP2. The sample chamber was set to 22° and 95% humidity.

Brown H.G, & Hanssen E. (2022). MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy. Communications Biology, 5, 817.

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