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Rabbit anti limk1

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Rabbit anti-LIMK1 is a primary antibody that specifically recognizes the LIMK1 protein. LIMK1 is a serine/threonine protein kinase that plays a role in the regulation of actin dynamics and cell motility.

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Lab products found in correlation

Rabbit anti cofilin, by Cell Signaling Technology (2 mentions) Rabbit anti phospho cofilin, by Cell Signaling Technology (2 mentions) Mouse anti ha antibody, by Fortrea (1 mentions) Bms 5, by Bristol-Myers Squibb (1 mentions) Rabbit anti β tubulin antibody, by Merck Group (1 mentions) Peroxidase conjugated goat anti rabbit and goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Y 27632, by R&D Systems (1 mentions) Bradford protein assay, by Bio-Rad (1 mentions) Puromycin, by Corning (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Mouse anti phospho cofilin, by Santa Cruz Biotechnology (1 mentions) Rabbit anti pak1, by Cell Signaling Technology (1 mentions) Rabbit anti pak4, by Cell Signaling Technology (1 mentions) Mouse anti actin, by Merck Group (1 mentions) Rabbit anti fmrp, by Abcam (1 mentions) Mouse anti rac1, by Merck Group (1 mentions) Mouse anti sv2, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti psd95, by Cell Signaling Technology (1 mentions) Mouse anti gapdh, by Biosynth (1 mentions) Alexa fluor phalloidin, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

LIMK1 (12 citations) Anti-LIMK1 (5 citations)

4 protocols using rabbit anti limk1

1

LIMK Inhibition in Cellular Studies

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Compound T5601640 (defined here as T56-LIMKi) was purchased from Ambinter (Paris, France). The LIMK inhibitor BMS-5 (Bristol-Myers Squibb) was purchased from SYNkinase (Shanghai, China). ROCK inhibitor Y-27632 was purchased from R&D Systems (Minneapolis, MN). The antibodies used were rabbit anti cofilin , rabbit anti phospo-cofilin (Ser3) and rabbit anti LIMK1 (Cell Signaling Technology, Beverly, MA); rabbit anti-β-tubulin antibody (Sigma-Aldrich, St. Louis, MO); mouse anti-HA antibody (Covance, Berkeley, CA); rabbit anti-LIMK2 (Abcam, Cambridge, MA ); peroxidase-conjugated goat anti-rabbit and goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA).
Rak R., Haklai R., Elad-Tzfadia G., Wolfson H.J., Carmeli S, & Kloog Y. (2014). Novel LIMK2 Inhibitor Blocks Panc-1 Tumor Growth in a mouse xenograft model. Oncoscience, 1(1), 39-48.
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2

Keratinocyte Serum-Free Medium Optimization

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Keratinocyte serum-free medium (K-SFM), bovine pituitary extract (BPE), epidermal growth factor (EGF), and 100 × antibiotic-antimycotic mixture were purchased from Life Technologies, Inc. (Grand Island, NY). miR-143 precursor vector construct and scramble miRNA vectors were purchased from SBI System Biosciences (Mountain View, CA). Puromycin was purchased from Cellgro (Manassas, VA). Rabbit anti-LIMK1 was purchased from Cell Signaling Technology (Beverly, MA). Mouse anti-phospho-Cofilin was purchased from Santa Cruz Biotech. Inc. (Santa Cruz, CA), and mouse anti-β-actin was purchased from Sigma Aldrich (St. Louis, MO). Horseradish peroxidase-conjugated mouse and rabbit secondary antibodies were purchased from Santa Cruz Biotech. Inc. (Santa Cruz, CA), and Bradford Protein Assay came from Bio-Rad Laboratories (Hercules, CA).
Ngalame N.N., Makia N.L., Waalkes M.P, & Tokar E.J. (2015). Mitigation of arsenic-induced acquired cancer phenotype in prostate cancer stem cells by miR-143 restoration. Toxicology and applied pharmacology, 312, 11-18.
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3

Synaptic Protein Regulation Analysis

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The following antibodies were used in this study: rabbit anti-phospho-cofilin (Ser3; 1:1000; Cell Signaling), rabbit anti-cofilin (1:1000; Cell Signaling Technology), rabbit anti-phospho-LIMK1 (Thr508; 1:1000; Abcam), rabbit anti-LIMK1 (1:1000; Cell Signaling Technology), rabbit anti-phospho-Slingshot1 (Ser978; 1:1000; ECM Biosciences), rabbit anti-Slingshot1 (1:1000; Abcam), rabbit anti–phospho-PAK1 (Ser199; 1:1000; Abcam), rabbit anti–phospho-PAK1 (Thr423; 1:1000; Cell Signaling Technology), rabbit anti-PAK1 (1:1000; Cell Signaling Technology), mouse anti-phospho-PAK4 (Ser474; 1:1000; Santa Cruz Biotechnology), rabbit anti-PAK4 (1:1000; Cell Signaling Technology), mouse anti-Rac1 (1:1000; Millipore), mouse anti-GAPDH (1:50,000; Fitzgerald), mouse anti-actin (1:10,000; Sigma-Aldrich), rabbit anti-FMRP (1:1000; Abcam), rabbit anti-PSD95 (1:1000; Cell Signaling Technology), mouse anti-SV2 (1:2000; Developmental Studies Hybridoma Bank), mouse anti-VAMP2 (1:1000; Thermo Fisher Scientific), rabbit histone H3 (1:1000; Cell Signaling Technology), horseradish peroxidase (HRP)-linked rabbit anti-immunoglobulin G (IgG) (1:5000; Cell Signaling Technology), and HRP-linked mouse anti-IgG (1:5000; Cell Signaling Technology).
Pyronneau A., He Q., Hwang J.Y., Porch M., Contractor A, & Zukin R.S. (2017). Aberrant Rac1-cofilin signaling mediates defects in dendritic spines, synaptic function, and sensory perception in fragile X syndrome. Science signaling, 10(504), eaan0852.
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4

Immunoblotting Antibody Cocktail Protocol

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Anti-cortactin (Clone 4F11), rabbit anti-p34Arc, anti-phospho-tyrosine (Clone 4G10), rabbit anti-TKS5 and mouse anti-MT1-MMP monoclonal antibodies were obtained from Millipore. Monoclonal anti-p34Arc was purchased from Synaptic System. Rabbit anti-Rab7, rabbit anti-cofilin, rabbit anti-phospho-cofilin, rabbit anti-LIMK1 and rabbit anti-LIMK2 antibodies were purchased from Cell Signaling. Rabbit anti-GADPH antibodies were purchased from Santa Cruz. AlexaFluor–phalloidin was from Invitrogen. Horseradish peroxidase-conjugated and fluorescently conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories.
Lagoutte E., Villeneuve C., Lafanechère L., Wells C.M., Jones G.E., Chavrier P, & Rossé C. (2016). LIMK Regulates Tumor-Cell Invasion and Matrix Degradation Through Tyrosine Phosphorylation of MT1-MMP. Scientific Reports, 6, 24925.
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