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Neutral buffered 10 formalin solution

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Neutral buffered 10% formalin solution is a commonly used fixative and preservative for tissue samples in various laboratory applications. It is a 10% formaldehyde solution that is buffered to a neutral pH to maintain the structural integrity of the samples during storage and processing.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Ham s f 12 nutrient mix, by Thermo Fisher Scientific (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Bovine insulin, by Merck Group (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions) Linoleic acid, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Glycerol, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) D glucose, by Thermo Fisher Scientific (1 mentions) Dulbecco s pbs, by Thermo Fisher Scientific (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Isoproterenol, by Merck Group (1 mentions) Keratinocyte sfm medium, by Thermo Fisher Scientific (1 mentions) L serine, by Merck Group (1 mentions) Arachidonic acid, by Merck Group (1 mentions) Gentamicin, by Merck Group (1 mentions) Mupirocin, by Merck Group (1 mentions)

Close spelling variants of this product

Formalin (867 citations) Neutral buffered formalin (592 citations) Formalin solution (199 citations) Buffered formalin (182 citations) Neutral buffered formalin solution (153 citations) Buffered formalin solution (16 citations) Neutral formalin (16 citations) Neutral buffered 10% formalin (11 citations) Formalin 10% (9 citations) Neutral formalin solution (7 citations)

15 protocols using neutral buffered 10 formalin solution

1

Optimized Cell Culture Media Formulations

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Tryptic soy broth (TSB), Luria broth (LB), D-glucose, phosphate-buffered saline (PBS), Dulbecco’s PBS (DPBS), Keratinocyte-SFM medium, DMEM (high glucose, GlutaMAX™ Supplement, pyruvate), and Ham’s F-12 Nutrient Mix were purchased from ThermoFisher Scientific (Waltham, MA). DermaLife K Keratinocyte Complete Medium with LifeFactors was obtained from Lifeline Cell Technology (Oceanside, CA). CnT-Prime 3D Barrier Medium was purchased from CELLnTEC Advanced Cell Systems AG (Zurich, Switzerland). Gentamicin, lysostaphin trimethoprim, sucrose, glycerol, mupirocin, neutral-buffered formalin solution (10%), and various supplements for skin culture media including hydrocortisone, isoproterenol, bovine insulin, selenious acid, L-serine, L-carnitine, bovine serum albumin (BSA), palmitic acid, linoleic acid, and arachidonic acid were obtained from Sigma-Aldrich (St. Louis, MI).
Wu B.(., Haney E.F., Akhoundsadegh N., Pletzer D., Trimble M.J., Adriaans A.E., Nibbering P.H, & Hancock R.E. (2021). Human organoid biofilm model for assessing antibiofilm activity of novel agents. NPJ Biofilms and Microbiomes, 7, 8.
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2

Thioacetamide-Induced Liver Injury Model

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Thioacetamide (TAA) was bought from Sigma-Aldrich (Cat. #: 163678, St. Louis, MO, USA). Lycorine hydrochloride was purchased from Molport® (Compound #: MolPort-009-653-412, Riga Latvia). Neutral buffered formalin solution 10% (Cat #: HT501128) was obtained from Sigma-Aldrich, St. Louis, MO, USA. In addition, phosphate-buffered saline (PBS) was purchased as a powder (pH 7.4) to prepare 1 L solutions (Cat. #: P3813, Sigma-Aldrich, MO, USA).
Alkreathy H.M, & Esmat A. (2022). Lycorine Ameliorates Thioacetamide-Induced Hepatic Fibrosis in Rats: Emphasis on Antioxidant, Anti-Inflammatory, and STAT3 Inhibition Effects. Pharmaceuticals, 15(3), 369.
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3

Histological Processing and Evaluation

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Tissues no more than 0.5 cm thick were fixed by immersion in Formalin Solution, Neutral buffered 10% (Sigma Aldrich, St. Louis, MO, USA) for at least 3 days and then put in 70% ethanol (Sigma Aldrich, St. Louis, MO, USA). Fixed samples were processed routinely, embedded in paraffin, and sectioned at 4 μm thickness. Sections were then stained with hematoxylin and eosin (H&E) using standard methods. All samples were reviewed by a board-certified veterinary pathologist. Findings were classified using the (International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) monograph.41 (link) All sections were examined using a BX11 microscope (Olympus, Tokyo, Japan).
Ferla R., Alliegro M., Dell’Anno M., Nusco E., Cullen J.M., Smith S.N., Wolfsberg T.G., O’Donnell P., Wang P., Nguyen A.D., Chandler R.J., Chen Z., Burgess S.M., Vite C.H., Haskins M.E., Venditti C.P, & Auricchio A. (2020). Low incidence of hepatocellular carcinoma in mice and cats treated with systemic adeno-associated viral vectors. Molecular Therapy. Methods & Clinical Development, 20, 247-257.
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4

Subgingival Plaque Biofilm Cultivation

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Subgingival plaque was collected from hopeless elements extracted for periodontal reasons and with probing greater than 7 mm. Plaque was incubated in 2 mL of LB Broth (Lennox L-broth base, Invitrogen, MA, USA), a generic culture medium for bacteria, for 24 h at 37 °C in oscillating incubator to obtain the inoculum for biofilm cultivation. Discs were placed into wells of 6-well cell plates (Corning Life Sciences, Woburn, MA, USA), covered with 3 mL of subgingival human plaque suspension + 2 mL of fresh LB broth medium. Bacteria and discs finally were incubated for 7 days, at 37 °C. The LB medium was replaced every 24 h. After 7 days of cultivation, the medium rich in bacteria was removed, and the biofilm-covered discs were transferred into new wells of a sterile 6-well plate. All discs were washed with PBS 1X, then discs used for Cell culture were leaved in PBS 1X, whereas discs used for microscopy were fixed in formalin solution neutral buffered 10% (Sigma Aldrich, MO, USA) for 15 min at room temperature and dehydrated using solutions with an increased concentration of alcohol (50%, 5 min—75%, 5 min—80%, 5 min, 95%, 5 min and 100%, 30 min). Fixed and non-fixed discs were stored at 4 °C.
Gianfreda F., Bollero P., Muzzi M., Di Giulio A., Nicolai E, & Canullo L. (2022). The Effects of Ultrasonic Scaling and Air-Abrasive Powders on the Decontamination of 9 Implant-Abutment Surfaces: Scanning Electron Analysis and In Vitro Study. Dentistry Journal, 10(3), 36.
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5

Immunohistochemical Analysis of Murine Spleen

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Spleens from mice were collected, fixed using Formalin solution neutral buffered 10% (Sigma-Aldrich, Missouri, USA), and soaked in 30% sucrose before being embedded in FSC 22 Frozen Section Media (Leica Biosystems, IL, USA) followed by snap freezing with dry ice. Tissue sectioning was done by the Laboratory Animal Research Facility in National Cancer Center, Korea. 6 μm-thick-sections were fixed for 15 minutes in acetone and methanol with a 1:1 ratio before blocked in PBS containing 10% FBS (Atlas Biologicals, CO, USA) and 0.3% TWEEN 20 (Sigma-Aldrich, Missouri, USA) for 1 hour at room temperature. Staining with mixture of 5 μg /ml FITC conjugated anti-B220 (clone RA3-6B2, Biolegend, CA, USA) and 20 μg/ml biotinylated anti-AID (clone mAID-2, eBioscience, CA, USA) was carried out at 4oC, overnight. Sections were washed and incubated with 1/500 dilution of Cyanine3 Streptavidin (Biolegend, CA, USA) at room temperature for 1 hour before counterstaining with DAPI. Sections were mounted in VECTASHIELD Antifade mounting medium (Vector Laboratories, CA, USA) and viewed with Olympus iX83 microscope. For H&E staining, fixed sections were stained using the H&E staining kit (Abcam, Cambridge, United Kingdom) following the manufacture’s instruction.
Nguyen H.T., Guevarra R.B., Magez S, & Radwanska M. (2021). Single-cell transcriptome profiling and the use of AID deficient mice reveal that B cell activation combined with antibody class switch recombination and somatic hypermutation do not benefit the control of experimental trypanosomosis. PLoS Pathogens, 17(11), e1010026.
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6

Analytical Techniques for Zearalenone

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Zearalenone (ZEN), α and β zearalenol (ZEL), α and β zearalanol (ZAL), hypothemycin, 17 β-estradiol (E2), dimethylsulfoxide (DMSO), penicillin/streptomycin (P/S), Dulbecco's phosphate buffered saline (PBS) and formalin solution neutral buffered 10% were purchased from Sigma Aldrich (Buchs, Switzerland); Dulbecco's Minimum Essential Medium (DMEM) high glucose with stable glutamine from PAA (Linz, Austria), charcoal stripped fetal bovine serum (FBS) and Hoechst 33258 were from Invitrogen (Eugene, Oregon, USA) and G418 from Roche (Mannheim, Germany).
Ehrlich V.A., Dellafiora L., Mollergues J., Dall'Asta C., Serrant P., Marin-Kuan M., Lo Piparo E., Schilter B, & Cozzini P. (2015). Hazard assessment through hybrid in vitro / in silico approach: The case of zearalenone. ALTEX, 32(4).
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7

Tissue Fixation and Staining Protocol

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Tissues collected from mice or patients were fixed overnight in neutral buffered 10% formalin solution (Sigma-Aldrich, Belgium) and processed in the lab (H&E staining) or in the tissue core facility at Ghent University Hospital (immunohistochemistry).
De Thaye E., Van de Vijver K., Van der Meulen J., Taminau J., Wagemans G., Denys H., Van Dorpe J., Berx G., Ceelen W., Van Bocxlaer J, & De Wever O. (2020). Establishment and characterization of a cell line and patient-derived xenograft (PDX) from peritoneal metastasis of low-grade serous ovarian carcinoma. Scientific Reports, 10, 6688.
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8

Hepatotoxicity Evaluation with 3-Methylcholanthrene

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For chemical treatments, a 100-mg vial of 3-methylcholanthrene (3MC) was purchased from Sigma-Aldrich (St. Louis, MO, USA) at an HPLC purity of >97.5%. Dimethyl sulfoxide (DMSO) and CH223191 were also purchased from Sigma-Aldrich. One-hundred percent pure corn oil (CO) was purchased from a local grocer. A 10 mg/mL stock of 3MC was made and this solution was made fresh before injection and disposed of after 30 days. Liver sections for H&E staining were preserved in neutral buffered 10% formalin solution (Sigma-Aldrich), and liver sections for Oil Red O were suspended in VWR® Clear Frozen Section Compound (Radnor, PA, USA) to embed tissues for cryosectioning. The Infinity™ ALT Liquid Stable Reagent was purchased from Fisher Diagnostics (Middletown, VA, USA) for use in the in vitro determination of ALT activity in mouse serum.
Cho T.E., Bott D., Ahmed S., Hutin D., Gomez A., Tamblyn L., Zhou A.C., Watts T.H., Grant D.M, & Matthews J. (2019). 3-Methylcholanthrene Induces Chylous Ascites in TCDD-Inducible Poly-ADP-Ribose Polymerase (Tiparp) Knockout Mice. International Journal of Molecular Sciences, 20(9), 2312.
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9

Ophthalmic Pilocarpine Formulation Development

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TT was provided by Aurobindo Pharmaceuticals (Hyderabad, India). Span® surfactants, cholesterol, soy lecithin, glycerine, acetic acid, pilocarpine and neutral buffered 10% formalin solution were from Sigma–Aldrich (St. Louis, MO, USA). Glyceryl distearate (Gattefosse, Saint-Priest, France), Wecobee® M, a triglyceride derived from fully hardened palm kernel oil (conforms USP/NF monograph) from Stepan (Northfield, IL, USA) and isopropyl alcohol (IPA) from Merck (Darmstadt, Germany).
Rajabalaya R., Leen G., Chellian J., Chakravarthi S, & David S.R. (2016). Tolterodine Tartrate Proniosomal Gel Transdermal Delivery for Overactive Bladder. Pharmaceutics, 8(3), 27.
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10

Quantitative Angiogenesis Assay on CAM

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An ex ovo CAM assay was performed as previously described [42 (link),43 (link)]. Briefly, fertilized chicken eggs (Granjas Gibert SA) were incubated for 3 days in a humidified incubator at 37 °C. The entire egg content was then carefully transferred into a Petri dish (430167, Corning) and incubated for another 6 days. On embryonic day E9, sterile methylcellulose disks containing or without GC8 particles were carefully placed on the CAM. For each experimental condition, six specimens were used and 4 disks were placed on each membrane. The disks were prepared previously to the implantation day by drying 50 μL drops of a solution containing 1.5% (w/v) (hydroxypropyl)methyl cellulose (Sigma-Aldrich) in MilliQ water with or without homogeneously distributed GC8 particles on a Teflon surface [44 (link)]. After 3 more days of incubation (E12), embryos were humanely sacrificed by decapitation and CAM was immediately fixed with a neutral buffered 10% formalin solution (Sigma-Aldrich) for 30 min. Finally, the membranes containing the disks were excised and images were acquired with an Olympus MVX10 Macroscope. Angiogenesis stimulation was quantitatively measured by counting the vessels converging towards the disks using ImageJ. A minimum of 10 samples were analyzed per condition.
Navarro-Requena C., Weaver J.D., Clark A.Y., Clift D.A., Pérez-Amodio S., Castaño Ó., Zhou D.W., García A.J, & Engel E. (2017). PEG Hydrogel Containing Calcium-Releasing Particles and Mesenchymal Stromal Cells Promote Vessel Maturation. Acta biomaterialia, 67, 53-65.
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