Anti inos pe

Manufactured by Thermo Fisher Scientific

Anti-iNOS PE is a laboratory reagent used for the detection and quantification of inducible nitric oxide synthase (iNOS) in biological samples. It functions as a specific antibody that binds to iNOS, allowing for its identification and measurement.

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Lab products found in correlation

Anti ifn γ pe cy7, by BioLegend (1 mentions) Anti ly6c efluor 450, by Thermo Fisher Scientific (1 mentions) Anti ly6g percp, by BioLegend (1 mentions) Anti cd45 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti arg1 fitc, by R&D Systems (1 mentions) Cytofix cytoperm kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PE anti-mouse iNOS (clone CXNFT, INOS-PE Anti-iNOS

2 protocols using anti inos pe

Multicolor Flow Cytometry Panel for Immune Cell Profiling

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The following mAbs were used: anti-CD11c Alexa Fluor 700 (N418) or anti-DEC-205 PE-cy7 (205yekta), anti-MHCII eFluor 450 (M5/114.15.2), anti-CD40 PE (1C10), anti-CD86 FITC (CT-CD8b), anti-CD86 Brilliant violet 605 (GL1), anti-CD11b Alexa Fluor 647 (M1/70), anti-Ly6C Alexa Fluor 488 (HK1.4), anti-F480 Alexa Fluor 488 (BM8), anti-Ly6C eFluor 450 (HK1.4), and anti-iNOS PE (CXNFT) from eBioscience; anti-CD8α Alexa Fluor 700 or PerCP (53-6.7), anti-CD11b Alexa Fluor 700 (M1/70), anti-F4/80 Brilliant violet 605 (BM8), anti-Ly6G PerCP (1A8), anti-CXCR4 Brilliant violet 421 (1276F12), anti-CXCR2 PE/Cy7 (5E8/CXCR2), anti-TNF-α Brilliant violet 650 (MP6-XT22), anti-CD3 FITC (145-2C11), anti-CD49 APC (DX5), and anti-IFN-γ PECy7 (XMG1.2) from Biolegend; and anti-MHC-II PE (AF6-120.1), anti-CD8α PerCP (53-6), anti-CD11c APC (N418), anti-CD74 FITC (In-1), anti-IL-12p40/70 V450 (C15.6), and anti-IL-12p40/70 APC (C15.6) from BD Bioscience.

Ruiz-Rosado J.D., Olguín J.E., Juárez-Avelar I., Saavedra R., Terrazas L.I., Robledo-Avila F.H., Vazquez-Mendoza A., Fernández J., Satoskar A.R., Partida-Sánchez S, & Rodriguez-Sosa M. (2016). MIF Promotes Classical Activation and Conversion of Inflammatory Ly6Chigh Monocytes into TipDCs during Murine Toxoplasmosis. Mediators of Inflammation, 2016, 9101762.

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Microglia iNOS and Arg-1 Expression

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To determine the expression of inducible nitric oxide synthase (iNOS) and arginase-1 (Arg-1) by microglia, brain mononuclear cells were harvested as described in previous section. Cells were surface stained using anti-CD45-PE-Cy7 and anti-CD11b-BV421 prior to fixation/permeabilization using cytofix/cytoperm kit (eBioscience). Cells were then stained with anti-iNOS-PE (eBioscience) and anti-Arg-1-FITC (R&D Systems), as recommended by manufacturer’s protocol.

Chauhan P., Hu S., Sheng W.S., Prasad S, & Lokensgard J.R. (2017). Modulation of Microglial Cell Fcγ Receptor Expression Following Viral Brain Infection. Scientific Reports, 7, 41889.

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