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Zeb 1 4c4

Manufactured by Abnova
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The ZEB-1 (4C4) is a lab equipment product from Abnova. It is designed to perform a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation or interpretation.

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Lab products found in correlation

α sma, by Abcam (3 mentions) Phospho jak2 tyr1007 1008, by Cell Signaling Technology (2 mentions) Phospho erk1 2, by Cell Signaling Technology (2 mentions) Interleukin 6 (il 6), by Abcam (2 mentions) Nf κb, by Cell Signaling Technology (2 mentions) Tgf β1, by Abcam (2 mentions) Erk1 2 137f5, by Cell Signaling Technology (1 mentions) Cyclin d1 2922, by Cell Signaling Technology (1 mentions) Phospho akt 193h12, by Cell Signaling Technology (1 mentions) Aldh1a1, by Merck Group (1 mentions) Mmp 9, by Cell Signaling Technology (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Anti mouse igg hrp conjugate, by Promega (1 mentions) Lin28b, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp conjugate, by Promega (1 mentions) Tyr1007 1008, by Cell Signaling Technology (1 mentions)

3 protocols using zeb 1 4c4

1

Comprehensive Protein Analysis Protocol

Cited 1 time
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This has been performed as previously described [32 (link)]. Antibodies directed against alpha smooth muscle actin (α-SMA), Twist-1, Vimentin (RV202), N-cadherin, transforming growth factor beta 1 (TGF-β1), VEGF-A (ab46154) and IL-6 were purchased from Abcam (Cambridge, MA); Lin28B (D4H1), AKT (C73H10), p-AKT (Thr308), STAT3, pSTAT3-Tyr705 (D3A7), Snail (C15D3), E-cadherin (24E10), EpCam (UV1D9), JAK-2 (D2E12), phospho-JAK-2 (TYR1007/1008), Cyclin D1 (2922), Akt, phospho-Akt (193H12), NF-κB, ERK1/2 (137F5), phospho-ERK1/2, MMP-9, HIF-1α, Glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 14C10) and β-Actin from Cell Signaling (Danvers, MA); ZEB-1 (4C4) from Abnova (Taipei, Taiwan), ALDH1A1 from Sigma (CA, USA) and IL-8 (EpR1116(2)) from Origene (Rockville, MD, USA).
Al-Harbi B., Hendrayani S.F., Silva G, & Aboussekhra A. (2018). Let-7b inhibits cancer-promoting effects of breast cancer-associated fibroblasts through IL-8 repression. Oncotarget, 9(25), 17825-17838.
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2

Immunoblotting Analysis of Cellular Markers

Cited 1 time
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This has been performed as previously described [18 (link)]. Antibodies directed against alpha smooth muscle actin (α-SMA), Ki-67, transforming growth factor beta 1 (TGF-β1), Stromal-derived factor-1 (SDF-1), Twist-1, Vimentin (RV202), MMP-9, (ab38898), ATR (ab54793), N-cadherin and interleukin-6 (IL-6) were purchased from Abcam (Cambridge, MA); STAT3, pSTAT3-Tyr705 (D3A7), Snail (C15D3), E-cadherin (24E10), EpCAM (UV1D9), JAK-2 (D2E12) and phospho-JAK-2 (TYR1007/1008), CyclinD1 (2922), Akt, phospho-Akt (193H12), MMP-2 (4022), ERK1/2 (137F5) and phospho-ERK1/2 from Cell Signaling (Danvers, MA); ZEB-1 (4C4) from Abnova (Taipei, Taiwan); p21 (F-5), p53 (DO-1) and Glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335) were purchased from Santa Cruz (Santa Cruz, CA).
Al-Ansari M.M, & Aboussekhra A. (2018). ATR suppresses the pro-tumorigenic functions of breast stromal fibroblasts. Oncotarget, 9(78), 34681-34690.
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3

Protein Expression Analysis Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
This has been performed as previously described [28 (link)]. Antibodies directed against DNMT1 (ab19905), alpha smooth muscle actin (α-SMA), Ki-67, transforming growth factor beta 1 (TGF-β1), Stromal-derived factor-1 (SDF-1), Twist-1, Vimentin (RV202), AUF1 (ab50692), N-cadherin and interleukin-6 (IL-6) were purchased from Abcam (Cambridge, MA); STAT3, pSTAT3-Tyr705 (D3A7), Snail (C15D3), E-cadherin (24E10), EpCam (UV1D9), JAK-2 (D2E12) and phospho-JAK-2 (TYR1007/1008), Cyclin D1 (2922), Akt, phospho-Akt (193H12), Nanog, NF-κB, OCT-4, PTEN, BMI-1, Lin-28B, Sox-2, ERK1/2 (137F5), phosph-ERK1/2 Glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335) from Cell Signaling (Danvers, MA); ZEB-1 (4C4) from Abnova (Taipei, Taiwan); p21 (F-5), p53 (DO-1), HuR (3A2), CD44 (Sp37) and CD24 (C-20) were purchased from Santa Cruz (Santa Cruz, CA), p16 (BD) was purchased from BD Biosciences (UK). Secondary antibodies are anti-Rabbit IgG HRP Conjugate (REF#W401), anti-mouse IgG HRP Conjugate (REF W402) and donkey anti-Goat IgG (H+L), HRP Conjugate (REF V805), from Promega (USA).
Al-Kharashi L.A., Al-Mohanna F.H., Tulbah A, & Aboussekhra A. (2017). The DNA methyl-transferase protein DNMT1 enhances tumor-promoting properties of breast stromal fibroblasts. Oncotarget, 9(2), 2329-2343.
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