CPT-11 (Irinotecan) was purchased from the Pfizer Pty Limited (Perth, Australia). LZ-8 was isolated from LZDCH (Yeastern Biotech, Taipei, Taiwan), which was extracted form Ganoderma lucidum, as described previously [24 (link), 25 (link)]. Briefly, LZDCH was dissolved and centrifuged, and proteins were obtained by filtration. LZ-8 was purified by fast protein liquid chromatography (GE Healthcare, Chicago, IL, USA).
Cpt 11
Manufactured by Pfizer
Sourced in Australia
CPT-11 is a laboratory product used for research purposes. It is a chemical compound that functions as a topoisomerase I inhibitor. The core function of CPT-11 is to inhibit the activity of topoisomerase I, an enzyme involved in the regulation of DNA topology.
Automatically generated - may contain errors
4 protocols using cpt 11
1
Purification of LZ-8 from Ganoderma lucidum
2
Establishment of Colon Carcinoma Model in Rats
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Animal use was approved by the Animal Care and Use Committee of the University of Alberta and conducted in accordance with Guidelines of the Canadian Council on Animal Care (AC08153). Female Fisher 344 rats (150–180 g of body weight and 11–12 weeks of age) were obtained from Charles River (QC, Canada). The use of female animals avoided potential confounding effects of sex, and allowed direct comparison with prior work in the same tumor model [13] (link), [14] (link). Rats were housed 2 per cage in a temperature (22°C) and light controlled (12 h light) room; water and food were available ad libitum. One week before chemotherapy, rats were separated into individual cages. The Ward colon carcinoma was provided by Dr. Y Rustum, Roswell Park Institute. Tumor pieces (0.05 g) were transplanted subcutaneously on the flank of the rats via trocar under slight isoflurane anesthesia. CPT-11 was provided by Pfizer as a ready-to-use clinical formulation. Atropine (0.6 mg/ml) was a clinical injectable formulation. Rats were killed by CO2 asphyxiation.
3
Synthesis and Procurement of Anticancer Agents
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TAK-931 was synthesized by Takeda Pharmaceutical Company Ltd. (23 (link)). Niraparib was purchased from Selleck China (Shanghai, China). Gemcitabine was purchased from Eli Lilly and Company (Indianapolis, IN, USA). CPT-11 (irinotecan hydrochloride injection) was purchased from Pfizer (Perth) Pty Limited (Bentley, WA, Australia). 5-FU (fluorouracil injection; 25 mg/ml) was purchased from Shanghai Xudong Haipu Pharmaceutical Inc. (Shanghai, China).
4
Cell Migration and Invasion Assays with Anticancer Drugs
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Cell migration assay was performed in 24-well CIM plates (BD Biosciences, CA, USA). Briefly, 1–3 × 104 cells per well were seeded in serum-free medium plus indicated drugs in the upper compartment of the CIM plates. Serum-complemented medium was added to the lower compartment of the chamber. After 24 h incubation, cells that passed through the septum were fixed with cold methanol and stained with crystal violet. The average number of migrated cells in four random microscopic fields was counted. Cell invasion assay was performed in 24-well CIM plates coated with matrigel. Other steps were identical to those of cell migration assay. VP-16 was provided by Hengrui Medicine (Jiangsu, China), ADM and CPT-11 were provided by Pfizer (New York, NY, USA). Fludarabine (HY-B0069), Bay11–7082 (HY-13453), C-176 (HY-112906), KU55933 (HY-12016) and AG490 (HY-12000) were purchased from MCE (Middlesex County, NJ, USA). GSH was purchased from Beyotime (Beijing, China).
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