Wst cell proliferation assay kit

Materials for the preparation of the hydrogels, acrylamide (AAm, 40% w/v), N,N′-methylenebisacrylamide (Bis, 2% w/v), alginic acid (sodium salt, low viscosity), ammonium persulfate (APS), N,N,N′,N′-tetramethylethylenediamine (TEMED), and calcium chloride (CaCl₂) were purchased from Sigma Aldrich (Saint Louis, MO, USA), and agarose (low melting) was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and used as received. Tris-HCl buffer (pH 7.2) was purchased from Life Technologies (Carlsbad, CA, USA) and binzil silica nanoparticle colloid solution with a mean particle size of 4 nm was a gift from AkzoNobel Pulp and Performance Chemicals Inc. (Marietta, GA, USA). 5-FU was purchased from Sigma (Saint Louis, MO, USA). Human U-87 glioblastoma cells were obtained from ATCC (Manassas, VA, USA), Dulbecco’s modified Eagle medium (DMEM) from Mediatech (Manassas, VA, USA), fetal bovine serum and penicillin-streptomycin from Invitrogen (Carlsbad, CA, USA), and sodium pyruvate, MEM non-essential amino acids and GlutaMax from Life Technologies (Carlsbad, CA, USA). WST cell proliferation assay kit was purchased from Dojindo Molecular Technologies (Rockville, MD, USA).

Human U-87 and U-251 glioblastoma cells were obtained from ATCC (Manassas, VA, USA); Dulbecco’s modified Eagle medium (DMEM), from Mediatech (Manassas, VA, USA); fetal bovine serum (FBS) and penicillin–streptomycin, from Invitrogen (Carlsbad, CA, USA); poly-L-lysine solution (0.01%), from Sigma Aldrich (Saint Louis, MO, USA); sodium pyruvate, MEM non-essential amino acids, and GlutaMax, from Life Technologies (Carlsbad, CA, USA); and trypsin, from CellGro (Manassas, VA, USA). A WST cell proliferation assay kit was purchased from Dojindo Molecular Technologies (Rockville, MD, USA). High-viscosity alginic acid sodium salt from brown algae (alginate) was purchased from Sigma Aldrich (Saint Louis, MO, USA); sterile alginate covalently coupled with GRGDSP, from FMC BioPolymer (Philadelphia, PA, USA); rat-tail collagen, from Advanced BioMatrix (San Diego, CA, USA); and agarose (low melting), from Thermo Fisher Scientific (Waltham, MA, USA), used as received. The chemical inhibitor of the RhoA-ROCK pathway (Y-27632) was purchased from Selleck Chemicals (Houston, TX, USA), and that of the Rac pathway (NSC23766), from EMD Biosciences (La Jolla, CA, USA). Integrin inhibitor RGD was purchased from Selleck Chemicals (Houston, TX, USA); GRGDSP, from Sigma Aldrich (Saint Louis, MO, USA); and cilengitide, from MedChem Express (Monmouth Junction, NJ, USA).