Malvern zetasizer nano instrument

The average particle size and the polydispersity were measured by photon correlation spectroscopy (PCS) using a Malvern zetasizer nano instrument (Malvern Instruments, Herrenberg, Germany). The resulting particle suspensions were diluted 1:100 with purified water and measured at a temperature of 22 °C using a backscattering angle of 173°.
The zeta potential was measured in the same instrument by laser Doppler microelectrophoresis to provide information about the surface charge of the nanoparticles. Thus, the nanoparticle dilutions described above were transferred into a folded capillary cell and the experiment was conducted at 22 °C.

DLS experiments were performed using a Malvern Zetasizer Nano instrument (Malvern Instruments Ltd, Worcestershire, UK) equipped with a He-Ne laser (633 nm) using backscattering mode (detector angle 173°). The CMS nanocarriers were dissolved in dH₂O, mixed by a Vortex shaker for 2 min, followed by filtration using a 0.45 μm RC syringe filter. 100 μL of the filtered solution was added to a disposable Plastibrand® micro cuvette (Brand GmbH + Co KG, Wertheim, Germany) with a round aperture. The autocorrelation functions of the backscattered light fluctuation were analyzed using Zetasizer DLS software from Malvern Instruments Ltd (Worcestershire, UK) to determine the size distribution by intensity and volume. The measurements were performed at 25 °C, equilibrating the system on this temperature for 120 s. Mean diameter values were obtained from four different runs.