The largest database of trusted experimental protocols

Oe snhg1

Manufactured by GenePharma
Sourced in China

The Oe-SNHG1 is a laboratory equipment product designed for research purposes. It is used to detect and analyze the expression levels of the SNHG1 gene. The core function of this product is to facilitate the quantification of SNHG1 gene expression in biological samples.

Automatically generated - may contain errors

3 protocols using oe snhg1

1

Lentiviral Knockdown and Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviruses expressing specific targeted knockdown SNHG1 [short hairpin-SNHG1 (sh-SNHG1)] and MDM2 (sh-MDM2) sequences and a scramble shRNA (sh-NC; control shRNA) were constructed by GenePharma (Shanghai, China) (Table 2). Lentiviruses overexpressing SNHG1 (oe-SNHG1), MDM2 (oe-MDM2), and PPARγ (oe-PPARγ), oe-NC, Inhibitor NC, and miR-9-3p inhibitor were obtained from GenePharma. Transfection was implemented using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).
+ Open protocol
+ Expand
2

Silencing SNHG1 via siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
The small interfering RNA against SNHG1 (sh-SNHG1), overexpression vector of SNHG1 (oe-SNHG1), miR-128-3p mimic/inhibitor and their negative control (sh-NC, oe-NC, NC mimic, NC inhibitor) were acquired from GenePharma (Shanghai, China). SH-SY5Y and SK-N-SH cells were transfected with aforementioned substances using Lipofectamine™ 3000 (Invitrogen, CA, USA) following the manufacturer’s instruction.
+ Open protocol
+ Expand
3

Lentiviral Knockdown and Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviruses expressing speci c targeted knockdown SNHG1 [short hairpin-SNHG1 (sh-SNHG1)] and MDM2 (sh-MDM2) sequences and a scramble shRNA (sh-NC; control shRNA) were constructed by GenePharma (Shanghai, China) (Table 2). Lentiviruses overexpressing SNHG1 (oe-SNHG1), MDM2 (oe-MDM2), and PPARγ (oe-PPARγ), oe-NC, Inhibitor NC, and miR-9-3p inhibitor were obtained from GenePharma. Transfection was implemented using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!