The human brain endothelial cell line (hCMEC/D3) was obtained under licence from University Paris 05, CNRS, Institute Cochin, INSERM (Paris, France). The cell line was maintained and characterised in accordance to regularly updated protocols by Institute Cochin and certified mycoplasma free56 (link). Human breast cancer cell line (MCF-7) (ATCC® HTB-22™) and human mammary epithelial cell line (MCF-10A) (ATCC® CRL-10317™) were purchased from American Type Culture Collection (VA, USA). Endothelial Basal Medium (EBM-2) (#CC-3156) was from Lonza Group Ltd. (Basel, Switzerland). Dulbecco/Vogt Modified Eagle's Minimal Essential Medium (DMEM) (#D0819), basic fibroblast growth factor (#F0291), gelatin solution (#G1393), hydrocortisone (#H-0888), insulin (#I-1882) and phosphate buffered saline (PBS) (#D8537) were purchased from Sigma-Aldrich Co. LLC. (Seelze, Germany). Foetal bovine serum (FBS) (#16000044) and penicillin/streptomycin (#15140122) were obtained from Gibco-BRL, Carlsbad Life Technologies (CA, USA). Recombinant human EGF (AF-100-15) was from PeproTech EC Ltd. (Hamburg, Germany). HEPES (#S11-001) was from PAA Cell Culture Company (Pasching, Austria). The cell culture flasks and plates were purchased from Corning Inc. (NY, USA).
Atcc crl 10317
Manufactured by American Type Culture Collection
Sourced in United States
ATCC® CRL-10317™ is a cell line derived from human embryonic stem cells. This product is intended for research use only and its core function is to serve as a model system for the study of human embryonic stem cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 10a, by American Type Culture Collection (3 mentions)
Hydrocortisone, by Merck Group (2 mentions)
Atcc htb 22, by American Type Culture Collection (2 mentions)
Gelatin solution, by Merck Group (1 mentions)
Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions)
Endothelial basal medium ebm 2, by Lonza (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
L 15 medium, by Merck Group (1 mentions)
Sk br 3, by Merck Group (1 mentions)
Cholera toxin, by Merck Group (1 mentions)
Mcf 10a, by Merck Group (1 mentions)
Atcc htb 19, by American Type Culture Collection (1 mentions)
Mda mb 436, by American Type Culture Collection (1 mentions)
Mda mb 436, by Merck Group (1 mentions)
Atcc crm htb 26, by American Type Culture Collection (1 mentions)
Mcf 7, by Merck Group (1 mentions)
Mda mb 231, by Merck Group (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
6 protocols using atcc crl 10317
1
Characterization of Human Cell Lines
2
Breast Cancer Cell Line Cultivation
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The human ER and PR positive BC cell line MCF-7 (ATCC® HTB-22™), HER-2 positive BC cell line SK-BR-3 (ATCC® HTB-30™), TNBC cell lines MDA-MB-436 (ATCC® HTB-130™), BT-20 (ATCC® HTB-19™) and MDA-MB-231 (ATCC® CRM-HTB-26™), and human non-cancerous mammary epithelial cell line (MCF-10A; ATCC® CRL-10317™) were purchased from the American Type Culture Collection (Manassas, USA). MDA-MB-231-luc cell line was a gift by Dr. Bin Gao, Ph.D., Professor from Chinese Academy of Sciences. MCF-7, SK-BR-3 and BT20 cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Sigma, USA) supplemented with 10% FBS, 100 U/mL penicillin and 50 U/mL streptomycin in a humidified atmosphere containing 5% CO2, at 37°C. MDA-MB-231 and MDA-MB-436 cells were maintained in L15 medium (Sigma, USA) supplemented with 10% FBS, 100 U/mL penicillin and 50 U/mL streptomycin in a humidified atmosphere at 37°C. MCF-10A cells were cultured in DMEM/Nutrient Mixture F-12 Ham medium (DMEM/F-12; Sigma, USA) containing 5% horse serum in the presence of 10 μg/mL insulin (Sigma, USA), 20 ng/mL epidermal growth factor (EGF; Sigma, USA), 100 ng/mL cholera toxins (Sigma, USA), 0.5 μg/mL hydrocortisone (Sigma, USA), 100 U/mL penicillin, and 50 U/mL streptomycin. All cells were used in experiments during the linear phase of growth.
3
Cell Culture of HCT116 and MCF10A
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The HCT116 and MCF10A cell lines were obtained from Horizon Discovery (HD PAR-007) and ATCC (ATCC CRL-10317™) respectively and grown in their respective recommended growth culture conditions.
4
Culturing Human Breast Cell Lines
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The (human breast epithelial adenocarcinoma MCF-7 WT P53) cell line was purchased from the American Type Culture Collection (ATCC-HTB-22™). The human normal epithelial breast cells (MCF-10A) was purchased from the American Type Culture Collection (ATCC-CRL-10317™). MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) 1,000 mg/L of glucose (Sigma-Aldrich D6046) containing 10% fetal bovine serum (Hyclone, SH30070.30) and 1% penicillin/streptomycin.
MCF-10A cells were cultured in mammary epithelial cell growth medium MEGM (Lonza, CC3151) along with the additives of Lonza clonetics kit (Lonza, CC4136) containing supplement and growth factors (bovine pituitary extracts, human epidermal growth factor, hydrocortisone, and insulin) without using the gentamycin amphotericin B mix provided with the kit.
MCF-10A cells were cultured in mammary epithelial cell growth medium MEGM (Lonza, CC3151) along with the additives of Lonza clonetics kit (Lonza, CC4136) containing supplement and growth factors (bovine pituitary extracts, human epidermal growth factor, hydrocortisone, and insulin) without using the gentamycin amphotericin B mix provided with the kit.
5
Evaluating Activated PRP Supernatants for Epithelial Cell Proliferation
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Supernatants of activated PRP was evaluated for their ability to stimulate proliferation of a human non-tumorigenic epithelial line (MCF 10A [ATCC® CRL-10317™], American Type Culture Collection, Manassas, VA, USA) [[57 (link),58 (link)] as previously described [37 ]. Briefly, MCF 10A cells seeded at 200,000 cells/cm2 in McCoy’s medium supplemented with 10% fetal bovine serum (Invitrogen, Grand Island, NY, USA), were grown for 24 hours at 37°C in 5% CO2 then washed twice with Hank’s Balanced Salt Solution (Invitrogen) and placed in serum-free media for an additional 24 hours. The serum-starved cells were then incubated for 24 hours at 37°C with control PPP (100 μL) from unactivated PRP or the supernatants of PEF- or thrombin-treated PRP. Cell proliferation was monitored by measuring total ATP/well using the ATPlite 1step single addition luminescence ATP detection assay (Perkin Elmer, Waltham, MA, USA) according to the manufacturer’s recommendations. Growth factor-dependent cell proliferation was confirmed by addition of purified recombinant human EGF (Lonza, Portsmouth, NH, USA).
6
Murine Mammary Cell Culture Protocols
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The TAC murine mammary epithelial cell line [32 (link), 33 (link)] was cultured on collagen-coated plates in Gibco high-glucose DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCS, penicillin (110 IU/ml), and streptomycin (110 μg/ml). A wild-type mouse mammary tumor (MMT) cell line (ATCC® CCL-51™; American Type Culture Collection, Manassas, VA, USA) was cultured in DMEM supplemented with 10% (vol/vol) FBS, gentamicin (100 IU/ml), and nonessential amino acids (Gibco; Thermo Fisher Scientific). The MCF10A cell line (ATCC® CRL-10317™; American Type Culture Collection) was propagated in DMEM/F-12 medium (Gibco; Thermo Fisher Scientific) supplemented with 5% horse serum, 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 500 ng/ml hydrocortisone, and 0.01 mg/ml insulin.
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