Macrophages were stained by DAPI simultaneously with CD68, HLA-DR, or CD206. Fibroblasts were stained by Actin, DAPI, Collagen Ⅰ antibody (1:100, Abcam, USA), vinculin (VCL) antibody (1:100, Abcam, USA), Integrin β1 antibody (1:100, Abcam, USA), and alpha-smooth muscle actin (α-SMA) antibody (1:100, Abcam, USA) for different purposes.
Integrin β1 antibody
Manufactured by Abcam
Sourced in United States
The Integrin β1 antibody is a tool used in research applications to detect and study the Integrin β1 protein. Integrin β1 is a transmembrane protein that plays a role in cell-cell and cell-extracellular matrix interactions. This antibody can be used to identify and quantify Integrin β1 expression in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using integrin β1 antibody
1
Macrophage and Fibroblast Immunostaining
2
Immunofluorescence Analysis of Cell-Cell Adhesion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After rehydration, tissue sections were blocked by goat serum, treated with hyaluronidase (0.8%) for 20 minutes at 37°C, and then incubated with N-CDH antibody, integrinβ1antibody (1 : 100, Abcam, UK), and YAP-TAZ antibody (1 : 100, Santa Cruz, USA) overnight at 4°C. Next, after an additional wash step, the sections were incubated with the fluorescent secondary antibody (1 : 1000; Proteintech, China) for 2 hours at room temperature, protected from light. The sections were then stained with DAPI and imaged using fluorescence microscopy (Leica, Germany).
3
Integrin β1 Expression in Laryngeal Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
From 2010 to 2016, 60 laryngeal cancer tissues and 25 noncancerous laryngeal tissues were obtained from patients who underwent surgery at Taihe hospital, Hubei University of Medicine. The tissues were incubated with integrin β1 antibody (1:300; Abcam, Cambridge, MA, USA) at 4 °C overnight. After washing in PBS, the tissues were incubated with HRP-labeled secondary antibody (Beyotime, Jiangsu, China) for 30 min at room temperature. Finally, the specific immunostaining was visualized using an ultrasensitive streptavidin-peroxidase system (Maxim Biotech, Fuzhou, China) [15 (link)]. The intensity of integrin β1 staining in individual cases was evaluated by two independent scorers in a blinded fashion. A score > 4 was defined as high expression, and a score ≤ 4 was regarded as low expression [16 (link)].
4
Immunofluorescence Staining of Integrin β1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The treated cells were grown on coverslips, fixed with 3% paraformaldehyde for 15 min and permeabilized with 0.5% Triton X‐100 in PBS for 10 min at room temperature and blocked with 3% goat serum for 20 min at room temperature. Then cells were incubated with integrin β1 antibody (1:500, abcam) overnight at 4°C. The next day, cells were washed in 0.1 mol/L PBS for 3 times and then incubated with fluorescein‐conjugated affinipure goat antirabbit IgG (1:500) for 3 hr at 37°C and subsequently stained the nuclei with DAPI. Finally, cells were observed using an Olympus BX51 fluorescence microscope (Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!