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5 protocols using polyethylene glycol 400

1

Formulation and Characterization of Nanoemulsion

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MNF standard was acquired from Sigma Aldrich (India), MNF API was procured from Yucca enterprises (India), Triethanolamine was procured from Fischer Scientific (Mumbai, India). Phospholipon 90 G was procured from Lipoid (Germany), Sodium cholate was procured from DC Fine Chemicals (India), Polyethylene glycol-400, Methanol, Chloroform and carbapol 934 P were acquired from SD fine chemicals (Mumbai, India). ELISA kits for inflammatory cytokines were acquired from ABclonal technology Co., Ltd. (India). Freund’s adjuvant, Complete cell suspension was procured from Sigma Aldrich (India). All HPLC solvents were acquired from SD Fine chemicals (Mumbai, India).
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2

Piperine-based Neuroprotective Formulation

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Piperine standard was acquired from Sigma Aldrich (Bangalore, India), Piperine API was procured from Yucca enterprises (Mumbai, India). Phospholipon 90G was procured from Lipoid (Ludwiashafen, Germany), sodium cholate was procured from DC Fine Chemicals (Mumbai, India), polyethylene glycol-400, methanol, chloroform span 60, and cholesterol were acquired from SD fine chemicals (Mumbai, India). Oxidative stress markers (superoxide dismutase, catalase, glutathione, malondialdehyde) kits were acquired from Abcam, Cambridge (UK). Pentylenetetrazole was procured from Sigma Aldrich (Bangalore, India). All HPLC solvents were acquired from SD Fine chemicals (Mumbai, India).
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3

Clomiphene Citrate-Krill Oil Formulation

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Clomiphene Citrate (CC) was a gift sample obtained from Palam Pharma Pvt. Ltd, Ahmedabad, India. Krill oil was purchased from Inlife Health Care supplements, Hyderabad, India. Labrasol (Caprylocaproyl Polyoxyl-8 glycerides) and Transcutol HP (Diethylene glycol monoethylether) were received as gift samples from Gattefosse Pvt. Ltd. (Mumbai, India). Tween 80, Tween 20, Span 80, Polyethylene glycol 400, polyethylene glycol 600, and propylene glycol were purchased from S.D. Fine chemicals (Mumbai, India). All of the other ingredients were procured commercially and were of analytical quality. Throughout the experiment, double distilled water was utilized.
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4

Raspberry Ketone Formulation Development

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Raspberry ketone was gift sample from the Kshipra Biotech, Indore, India. Sefsol 218 ® was provided as gift sample from Nikko Chemicals (Tokyo, Japan). The oil vitamin E was purchased from Merck, Mumbai, India. Olive oil and almond oil were purchased from Falcon, Bengaluru (India). Linseed oil was purchased from Sigma-Aldrich, Mumbai, India. Solutol HS 15 was provided as gift samples from Signet Chemicals Corporation Pvt. Ltd, Mumbai, India. Tween 20, tween 60, tween 80 and polyethylene glycol 400 were gifted samples from SD Fine Chemicals, New Delhi, India. Propylene glycol was procured from Thomas Baker Chemicals, Mumbai, India.Capmul MCM, lauroglycol 90 and transcutol P were obtained as gift from Gattefosse (Saint Priest, Cedex, France). HPLC-grade acetonitrile was purchased from Merck, Mumbai, India.
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5

Fisetin-loaded Nanoformulation Development

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Fisetin was purchased from Xi'an XIAOCAO Botanical Development Co., Ltd. (China). Lipoid S 100 was obtained from Lipoid GmbH (Ludwigshafen, Germany). Sodium cholate was purchased from Thomas Baker (Mumbai, India). Ethanol (absolute alcohol 99.9%) was purchased from Brampton, Ontario L6T 3Y4 (Canada). Chloroform, sodium hydroxide and potassium dihydrogen orthophosphate were purchased from Merck (Mumbai, India). Carbopol 934, triethanolamine and polyethylene glycol-400 were purchased from SD fine chemicals (Mumbai, India). All other chemicals and solvents used were of analytical grade. Water for high performance liquid chromatography (HPLC) was used for all experiments.
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