The PPMIP, BPAMIP, and NIP particles were slurry-packed into three HPLC columns (100 mm × 4.6 mm i.d.) using a HY-HPLC-S packing pump (Hydrosys Industries, Beijing, China) under 3000 psi with methanol as solvent, and the obtained HPLC columns were named as PP-imprinted, BPA-imprinted, and non-imprinted columns, according to the type of their packing materials. Chromatographic analyses were performed with an HPLC system equipped with a Rheodyne manual injector, a Waters 515 pump, and a Waters 2487 UV detector.
The chromatographic evaluation was performed by injecting 20 μL of each analyte (20 ppm) with a mobile phase (ACN) flow rate of 1 mL·min−1, and 220 nm was selected as the detection wavelength. The capacity factor (k) was calculated according to Equation (1):
where tR and t0 are the retention times of the analyte and the void marker (acetone), respectively. The IFs of PPMIP for BPA, BPB, BPAF, BPS, E2, DES, and NP were calculated by Equation (2), and the IFs of BPAMIP for these analytes were calculated by Equation (3):
where kPPMIP, kBPAMIP and kNIP are the capacity factors of the analytes on the PP-imprinted, BPA-imprinted, and non-imprinted columns, respectively.
The chromatographic evaluation was performed by injecting 20 μL of each analyte (20 ppm) with a mobile phase (ACN) flow rate of 1 mL·min−1, and 220 nm was selected as the detection wavelength. The capacity factor (k) was calculated according to Equation (1):
where tR and t0 are the retention times of the analyte and the void marker (acetone), respectively. The IFs of PPMIP for BPA, BPB, BPAF, BPS, E2, DES, and NP were calculated by Equation (2), and the IFs of BPAMIP for these analytes were calculated by Equation (3):
where kPPMIP, kBPAMIP and kNIP are the capacity factors of the analytes on the PP-imprinted, BPA-imprinted, and non-imprinted columns, respectively.