Log In Sign up
The largest database of trusted experimental protocols

Mhc 2 pe

Manufactured by BD
Visit Supplier
Sourced in United States

The MHC-II-PE is a fluorescently labeled antibody that binds to major histocompatibility complex class II (MHC-II) proteins. It is used in flow cytometry applications to identify and characterize cells expressing MHC-II on their surface.

Automatically generated - may contain errors

Lab products found in correlation

Cd11b fitc, by BD (2 mentions) Cd11c apc, by BD (2 mentions) Cd86 pe, by BD (2 mentions) Suramin, by Bio-Techne (1 mentions) Luminescent atp detection assay kit, by Abcam (1 mentions) Dynasore, by Merck Group (1 mentions) Fluo 4 direct calcium assay kit, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Ox atp, by Bio-Techne (1 mentions) Recombinant murine gm csf, by R&D Systems (1 mentions) Arl67156, by Bio-Techne (1 mentions) Bzatp, by Bio-Techne (1 mentions) Psb 12062, by Merck Group (1 mentions) F4 80 pe, by BD (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Cd11b fitc, by BioLegend (1 mentions) Ly6c bv421, by BD (1 mentions) Ly6g percp cy5, by BD (1 mentions)

Close spelling variants of this product

MHC-II (4 citations) PE-conjugated anti-MHC II (3 citations) Anti-MHC class II-PE-Cy5 (2 citations) Anti-mouse I-A/I-E (MHC II)-PE (2 citations) MHC II-PE-cy7 (2 citations)

8 protocols using mhc 2 pe

1

Murine Dendritic Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Female C57BL/6 mice purchased from Ji'nan Pengyue Laboratory Animal Breeding Co., Ltd. (China) were housed and maintained in the animal facilities of Weifang Medical University. Recombinant murine GM-CSF and IL-4 were purchased from R&D Systems (USA). A Fluo-4 Direct™ calcium assay kit was purchased from ThermoFisher Scientific (USA). ARL-67156, Bz-ATP, OX-ATP, and suramin were purchased from Tocris Bioscience (USA). A-438079, a competitive P2X7 receptor antagonist, was purchased from Santa Cruz (USA). Luminescent ATP Detection Assay kit was purchased from Abcam (USA). The endocytosis inhibitors MDC, PitS2, Dynasore, and P2X4-specific antagonist PSB-12062 were purchased from Sigma-Aldrich (USA). RNAiso Plus, SYBR Premix Ex Taq II, and the PrimeScriptTM RT reagent Kit were obtained from Takara (China). CD11c-APC (#550261), CD11b-FITC (#553310), MHC II-PE (#558593), and F4/80-PE (#565410) and isotype control antibodies were purchased from BD Bioscience (USA). CD39-PE (#12-0391-82) was purchased from Thermo Fisher (USA). All experiments involving animals were performed in accordance with the Chinese National Laboratory Animal-Guideline for Ethical Review of Animal Welfare and approved by the Institutional Animal Care and Use Committee of Weifang Medical University (NO. 010/2017).
Zhao R., Qiao J., Zhang X., Zhao Y., Meng X., Sun D, & Peng X. (2019). Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation. Frontiers in Immunology, 10, 2524.
+ Open protocol
+ Expand
2

Tumor Dissociation and Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumours were minced and digested in 3 mL RPMI with 2% Hepes, 2% FCS, 0.5 mg/mL Collagenase D and 0.1 mg/mL DNAse 1 at + 37 °C on a shaker (enzymes from Roche). After 45 min 300 μL 0.1 M EDTA was added for 5 min to stop the reaction. Single cell suspension was then obtained by using gentleMACS C tubes with a gentleMACS Dissociator and filtering of the suspension. Cells were blocked with BD’s FC-block (Cat# 553141) 30 min on ice, stained with directly conjugated antibodies from BD (30 min on ice) and a viability dye, recorded on a LSR Fortessa flow-cytometer (BD, BioSciences) and analysed with Flowjo v10 (FlowJo LLC). The used antibodies and dyes were: Fixable Viability Dye eFluor 780 (eBioscience, Cat# 65-0865-14), CD11b FITC (BioLegend, Cat# 101206), Ly6G PerCP-Cy5.5 (BD, Cat# 560602), F4/80 APC (eBiosicence, Cat# 17-4801-82), MHCII PE (BD, Cat# 557000), Ly6C-BV421 (BD, Cat# 562727).
Tuominen S., Keller T., Petruk N., López-Picón F., Eichin D., Löyttyniemi E., Verhassel A., Rajander J., Sandholm J., Tuomela J, & Grönroos T.J. (2020). Evaluation of [18F]F-DPA as a target for TSPO in head and neck cancer under normal conditions and after radiotherapy. European Journal of Nuclear Medicine and Molecular Imaging, 48(5), 1312-1326.
+ Open protocol
+ Expand
3

Multiparameter Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prior to flow cytometry, cells were washed in staining buffer (0.05% (w/v) BSA, 2 mM EDTA in 1× PBS) and treated with FcR blocking reagent (Miltenyi Biotec) for 10 min. Subsequently, In vitro differentiated single cell clones and Dox-pDC were stained with the following antibodies for 30 min at 4°C: CD11c-APC, MHC-I-FITC, MHC-II-PE, SiglecH-PE, CD86-PE-Cy7, CD289 (TLR9)-FITC, CD11b-V500, B220-PerCP, CD8α-APC-Cy7 (all BD Biosciences) and CD9-FITC (Thermo Fisher). T lymphocytes were stained with the following antibodies: CD3-FITC, CD4-V500, CD8α-APC-Cy7, CD44-APC and IFNγ-APC-Cy7 (all BD Biosciences); CD62L-PerCP-Cy5.5 and RORγt-PerCP-ef710 (all Thermo Fisher Scientific). Flow cytometry was performed using LSRII and FlowJo analysis software (V10; FlowJo, Ashland, USA).
Thieme S., Holzbaur A., Wiedemuth R., Binner A., Navratiel K., Anastassiadis K., Brenner S, & Richter C. (2018). The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile. PLoS ONE, 13(2), e0192437.
+ Open protocol
+ Expand
4

NLRP3 Regulates VSMC Apoptosis and Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols
After culturing in serum‐free medium for 24 hours, primary VSMCs from NLRP3fl/fl or NLRP3SMC KO mice were treated with 20% CKD serum for 48 hours. Then VSMCs were collected for Annexin V/PI staining (BD Biosciences, San Jose, CA) to quantify VSMC apoptosis or injected intraperitoneally (105 cells per C57BL/6 mice). Twelve hours later, the peritoneal lavage fluid was collected for flow cytometry. The following fluorochrome‐conjugated antibodies were used: F4/80‐fluorescein isothiocyanate (eBioscience 11‐4801‐82, 1:50 dilution), MHC II‐PE (BD Biosciences 553566, 1:50 dilution), CD40‐PerCP Cy5.5 (Biolegend 124623, 1:50 dilution), and CD80‐PE Cy7 (Biolegend 104733, 1:50 dilution).
Ding X., Chen J., Wu C., Wang G., Zhou C., Chen S., Wang K., Zhang A., Ye P., Wu J., Chen S., Zhang H., Xu K., Wang S, & Xia J. (2018). Nucleotide‐Binding Oligomerization Domain‐Like Receptor Protein 3 Deficiency in Vascular Smooth Muscle Cells Prevents Arteriovenous Fistula Failure Despite Chronic Kidney Disease. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(1), e011211.
+ Open protocol
+ Expand
5

Quantifying DC Activation Markers

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
DCs (0.5 × 106) were either left unstimulated or stimulated for 24 hours with bare M278 and PPM (each at 2.5 μg/mL) or an equivalent weight of PPP as used for PPM to quantify changes in expressions of CD80, CD86, CD40, MHC-I, and MHC-II as markers of differentiation and activation. DCs were washed, and Fc surface receptors were blocked for 15 minutes at 4°C using a purified mouse anti-CD16/CD32 receptor blocking antibody (BD Bioscience) diluted in fluorescent-activated cell sorting (FACS) buffer [phosphate-buffered saline (PBS) 0.1% NaN3, 1.0% FBS]. DCs were washed and stained with fluorochrome-conjugated antibodies against CD11c-APC-Cy7 (BD: 561241), MHC-I-A647 (BD: 562832) MHC-II-PE (BD: 558593), CD86-FITC (BD: 553691), CD80-PECy-7 (BD: 562504), CD40-BV421 (BD: 562846) each at 0.250 μg/100 μL. Unstimulated control cells (medium) were kept as background controls. Cells were then washed and fixed with 1% paraformaldehyde (PFA) solution for 20 minutes at 4°C. Data were acquired on a BD LSR II flow cytometer (BD Bioscience) with at least 1 × 105 events for each sample and analyzed using FlowJo software (Tree Star Inc., Ashland, OR, USA) [19 (link)].
Dixit S., Sahu R., Verma R., Duncan S., Giambartolomei G.H., Singh S.R, & Dennis V.A. (2017). Caveolin-Mediated Endocytosis of the Chlamydia M278 Outer Membrane Peptide Encapsulated in Poly(lactic acid)-Poly(ethylene glycol) Nanoparticles by Mouse Primary Dendritic Cells Enhances Specific Immune Effectors Mediated by MHC Class II and CD4+ T cells. Biomaterials, 159, 130-145.
+ Open protocol
+ Expand
6

Quantifying Immune Cell Populations in Epididymal Fat

Check if the same lab product or an alternative is used in the 5 most similar protocols
The dissected epididymal fat samples were minced in DMEM with 1 mg/ml collagenase P (Roche, Mannheim, Germany) and 5% BSA. After 45 min of shaking incubation at 37 °C, any debris was filtered using nylon mesh. The stromal vascular fraction (SVF) was separated via centrifugation (500 g for 10 min). The SVF was washed and incubated with antibodies for further flow cytometry analysis. For the analysis of macrophage population, the SVF was stained with following antibodies: CD11b-FITC (BD 557396, BD Biosciences, Franklin Lakes, NJ), F4/80-Bv421 (BD 565411), CD206-APC (BD 565250), and MHC II-PE (BD 562010). For the analysis of lymphocyte population, the SVF was stained with following antibodies: CD19-APC-Cy7 (BD 561043) and CD3-PE-Cy5 (BD 561108). Samples were analyzed using a BD FACSCanto and FACSDiva software (BD Biosciences, Franklin Lakes, NJ). Data were processed with FlowJo software (Tree Star Inc. Ashland, OR).
Ahn C.H., Choi E.H., Lee H., Lee W., Kim J.I, & Cho Y.M. (2021). Vertical sleeve gastrectomy induces distinctive transcriptomic responses in liver, fat and muscle. Scientific Reports, 11, 2310.
+ Open protocol
+ Expand
7

Influenza Vaccine Immunogenicity Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
AEAR was prepared in our lab [22 (link)]. 2014/2015 seasonal influenza virus split vaccine was purchased commercially from Shenzhen sanofi Pasteur biological products co., LTD (Shenzhen China). Goat anti-mouse peroxidase conjugates (IgG-HRP, IgG1-HRP and IgG2a-HRP) were purchased from Southern Biotech Inc. USA. Concanavalin A (ConA), lipopolysaccharide (LPS, from Escherichia coli 055:B5) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were from Sigma-Aldrich Co. LLC. USA. Imject Alum Adjuvant (Alum) was from Thermo Scientific Pierce USA. CD11c-FITC, CD3-PE, CD4-APC, CD8a-FITC, CD44-PE, CD25-APC, Foxp3-PE, IFN-γ-PE, IL-4-PE, CD86-PE, CD40-APC, CD80-APC, MHC-II-PE, Cytofix/Cytoperm and Perm/Wash buffer were purchased from BD Bioscience USA. Carboxyfluorescein diacetate succinimidyl ester (CFSE), Treg staining kit were from eBioscience USA. All the other reagents were analytically purified in China.
Zhang A., Wang D., Li J., Gao F, & Fan X. (2017). The effect of aqueous extract of Xinjiang Artemisia rupestris L. (an influenza virus vaccine adjuvant) on enhancing immune responses and reducing antigen dose required for immunity. PLoS ONE, 12(8), e0183720.
+ Open protocol
+ Expand
8

Microglia Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated microglia were suspended in an incubation buffer (50 μl; 1 × PBS + 0.1%BSA) for 30 min on ice then Fc receptors blocked with anti-CD32 (BD Bioscience, San Jose, CA). Fluorescent conjugated antibodies were applied on ice for 30 min in the dark to assess microglia purity (mouse anti-rat CD11b-FITC, BD Pharmingen, San Jose, CA; mouse anti-rat-CD45-APC, eBioscience, San Diego, CA) and state of activation (mouse anti-rat: MHC-II-PE, CD32-PE, CD86-PE; BD Bioscience, San Jose, CA). For CD206, cells were incubated in rabbit anti-rat CD206 then donkey anti-rabbit-PE secondary antibody (BD Bioscience, San Jose, CA). Following washes in PBS, cells were analyzed on an Attune Acoustic Focusing Cytometer (ABI, Carlsbad, CA) calibrated with commercially available beads prior to each run. Fluorescence spillover compensation values were generated from non-stained cell populations and single-color staining controls. Isotype controls were used to exclude the non-specific binding of antibodies. For each staining condition, 1 × 104 events were collected.
Peng H, & Nixon K. (2021). Microglia Phenotypes Following the Induction of Alcohol Dependence in Adolescent Rats. Alcoholism, clinical and experimental research, 45(1), 105-116.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!