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Synergi 4 m fusion rp 80 column

Manufactured by Phenomenex
Sourced in Germany

The Synergi™ 4 µm Fusion-RP 80 Å column is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a 4 µm particle size and 80 Å pore size. The column is designed for the separation and analysis of a variety of compounds.

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2 protocols using synergi 4 m fusion rp 80 column

1

Controlled Release of Vancomycin from Modified Collagen Sheets

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Collagen sheets were loaded with 1 mg of vancomycin in PBS containing 0.01% (w/v) RB and modified by RGX. Unmodified collagen sheets loaded with 1 mg of vancomycin in PBS were used as control. To investigate the release of vancomycin, collagen sheets were used directly after treatment loading and incubated in a 24-well plate in 1 mL of PBS at either RT or 37 °C. Samples were taken after 30 min, 1 h, 2 h, 4 h, 8 h and 24 h by agitating and withdrawing the entire supernatant and adding 1 mL of fresh PBS to the collagen sample. The samples were analyzed by reversed-phase HPLC on a Shimadzu LC20-AD system, SIL-20AC autosampler, SPD-M20A photodiode array detector and a C18 250 × 4.6 mm Synergi™ 4 µm Fusion-RP 80 Å column (Phenomenex, Aschaffenburg, Germany). Then, 100 µL of the supernatant was injected onto the column by autosampler. After washing for 20 min with eluent A (95% water, 5% acetonitrile, 0.1% TFA), the concentration of eluent B (5% water, 95% acetonitrile, 0.1% TFA) was steadily increased from 0% to 90% over 50 min at a flow rate of 0.5 mL/min. Absorption was monitored at 280 nm for vancomycin and at 220 nm for collagen. Vancomycin eluted after a retention time of 41 min. The amount of released vancomycin was calculated from a calibration curve of vancomycin from 0 to 1 mg/mL.
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2

Optimized HPLC Analytical Method Development

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The optimization tests were carried out on various reversed-phase high performance liquid chromatography (HPLC) analytical columns with different stationary phase compositions; Kinetex 2.6 µm XB-C18 100 Å (100 × 2.1 mm), Synergi 4 µm Polar-RP 80 Å (250 × 2 mm) and Synergi 4 µm Fusion-RP, 80 Å column (250 mm × 2 mm) (all provided by Phenomenex). Four different mobile phases (with different solvent gradient programs, column oven temperatures and flow rates) with different modifiers were tested: (I) acetonitrile-water with 0.1% acetic acid (II) acetonitrile-water with 0.1% formic acid (III) methanol-water with 0.1% acetic acid (IV) methanol-water with 0.1% formic acid. The best result was obtained with the chromatographic conditions described in Section 3.5.
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