3.5 μL of the membrane remodeling reactions were applied to glow-discharged R1.2/1.3 Quantifoil 200 Cu mesh grids (Quantifoil) in a Mark III Vitrobot (FEI). Grids were blotted with Whatman #1 filter paper (Whatman) for 4–8 seconds with a 0 mm offset at 19 °C and 100 % humidity before plunging into liquid ethane. Grids were stored under liquid nitrogen until samples were imaged for structural determination. Datasets were collected either on a 300 kV Technai Polara (membrane-bound CHMP1B+IST1 filaments), a 300 kV Titan Krios operating with an energy filter with a 20 eV slit (membrane-bound CHMP1B-only filaments), or a 200 kV Technai F20 (IST1-only filaments), all using a K2 Summit detector operated in super-resolution mode and binned by a factor of 2 for subsequent processing. Data collection parameters are summarized in Table 1 .
200 cu mesh grids
Manufactured by Quantifoil
The Quantifoil 200 Cu mesh grids are a type of specimen support grid used in electron microscopy. The grids feature a uniform pattern of holes or 'lacey' support film made of carbon, which is deposited on a copper mesh. The core function of these grids is to provide a stable and consistent platform for mounting and analyzing samples during electron microscopy imaging and analysis.
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Lab products found in correlation
2 protocols using 200 cu mesh grids
1
Cryo-EM Imaging of Membrane-Bound Protein Complexes
2
Cryo-EM Imaging of Membrane-Bound Protein Complexes
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3.5 μL of the membrane remodeling reactions were applied to glow-discharged R1.2/1.3 Quantifoil 200 Cu mesh grids (Quantifoil) in a Mark III Vitrobot (FEI). Grids were blotted with Whatman #1 filter paper (Whatman) for 4–8 seconds with a 0 mm offset at 19 °C and 100 % humidity before plunging into liquid ethane. Grids were stored under liquid nitrogen until samples were imaged for structural determination. Datasets were collected either on a 300 kV Technai Polara (membrane-bound CHMP1B+IST1 filaments), a 300 kV Titan Krios operating with an energy filter with a 20 eV slit (membrane-bound CHMP1B-only filaments), or a 200 kV Technai F20 (IST1-only filaments), all using a K2 Summit detector operated in super-resolution mode and binned by a factor of 2 for subsequent processing. Data collection parameters are summarized in Table 1 .
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