Fluorescent h2s probe 7 azido 4 methylcoumarin azmc

The CBS/CSE inhibitor AOAA (O-(carboxymethyl)hydroxylamine hemihydrochloride) [9 (link)] was purchased from Sigma-Aldrich (Burlington, MA, United States), the 3-MST inhibitor HMPSNE (2-[(4-hydroxy-6-methylpyrimidin-2-yl)sulfanyl]-1-(naphthalen-1-yl)ethan-1-one) [10 (link)] was purchased from MolPort (Riga, Latvia). The fluorescent H₂S probe 7-azido-4-methylcoumarin (AzMC) was obtained from Sigma-Aldrich (Burlington, MA, USA).
Rabbit monoclonal anti-CBS (D8F2P), anti-ATP citrate lyase (ACLY) (D1 × 6P), anti-β-catenin (D10A8), anti-mouse IgG HRP-linked antibody (#7076) and rabbit polyclonal anti-phospho-ACLY (Ser455) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-β-actin (AC-15) and rabbit polyclonal anti-SQR (HPA017079) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Anti-rabbit IgG (H+L) cross-adsorbed secondary antibody-HRP (#31458) was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal anti-3-MST (ab224043), anti-CSE (ab151769), anti-TST (ab231248) and rabbit monoclonal anti-ETHE1 (ab174302) were purchased from Abcam (Cambridge, UK).

The cell proliferation ELISA 5-bromo-2′-deoxyuridine (BrdU) kit was purchased from Roche Diagnostics Ltd. (Sigma–Aldrich Chemie Gmbh: Munich, Germany). The β -actin (8H10D10) mouse monoclonal antibody (#3700), the Tom20 (D8T4N) rabbit monoclonal antibody (#42406), the ELISA Lysis Buffer (1X), the protease/phosphatase inhibitor cocktail (100X), and horseradish peroxidase (HRP)-linked secondary antibodies against the corresponding species IgG of the primary antibodies were obtained from Cell Signaling Technology (Bioconcept AG: Allschwill, Switzerland). The 3-MST rabbit polyclonal antibody (ab85377) and the Amersham ECL Prime Western blotting detection reagent kit were obtained from Abcam PLC (Cambridge, UK) and GE Healthcare Life Sciences (Sigma–Aldrich Chemie Gmbh: Munich, Germany), respectively. The mitochondrial isolation kit MITOISO2, the fluorescent H₂S probe 7-azido-4-methylcoumarin (AzMC) [10 (link)], the fluorescent polysulfide probe (E)-2-(3-(6-(2-hydroxyethylamino)naphthalen-2-yl)-3-oxoprop-1-enyl)-3,5-dimethoxybenzaldehyde (P3) [11 (link)], bovine serum albumin (BSA), and lactalbumin hydrolysate were purchased from SigmaAldrich Chemie Gmbh (Munich, Germany). All other materials and reagents for cell culture and Western blotting were obtained from Thermo Fisher Scientific (Basel, Switzerland), unless otherwise stated.