Four-armed polyethylene glycol 2000 Da (star-PEG) was purchased from Qiyue Biotechnology Co., Ltd (Xi'an, China). CL monomers, Sn(Oct)2, alvespimycin (17-DMAG) HCl, poly (vinyl pyrrolidone) (PVP, K30), potassium hexacyanoferrate (III) (K3 [Fe(CN)6]), and hyaluronic acid (HA, 20 KDa) were bought from Macklin Co. (Shanghai, China). Fetal bovine serum (FBS), RPMI 1640 medium, cell counting kit-8 (CCK-8), calcein AM/PI cell viability assay kit, annexin V-FITC apoptosis detection kit, cell cycle and apoptosis analysis kit and DCFH-DA were also acquired from Meilun Biotechnology Co. (Dalian, China). The cyclin A2 rabbit monoclonal antibody, Akt, phospho-Akt (Ser 473), HSP90-1α, HSP70 and GPX4 rabbit polyclonal antibody were purchased from the Beyotime Biotechnology Co. (Haimen, China). ACSL4 mouse monoclonal antibody were bought from Sigma-Aldrich Co. (USA).
Annexin 5 fitc apoptosis detection kit
Manufactured by Meilun
Sourced in China
The Annexin V-FITC Apoptosis Detection Kit is a laboratory reagent used for the detection and quantification of apoptosis, a programmed cell death process. The kit contains Annexin V, a protein that binds to phosphatidylserine, a molecule that is externalized during the early stages of apoptosis. Annexin V is conjugated to the fluorescent dye FITC, allowing for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Meilun (2 mentions)
Cell counting kit 8 cck 8, by Meilun (2 mentions)
Cell cycle and apoptosis analysis kit, by Meilun (1 mentions)
Dcfh da, by Meilun (1 mentions)
Hsp70, by Beyotime (1 mentions)
Gpx4 rabbit polyclonal antibody, by Beyotime (1 mentions)
Rpmi 1640 medium, by Meilun (1 mentions)
Methoxamine, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rnase a, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Yeasen (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Bca protein assay kit, by Yeasen (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Cytoflex s, by Beckman Coulter (1 mentions)
Coq10, by Merck Group (1 mentions)
Hydroxyproline assay kit, by Nanjing Jiancheng (1 mentions)
Tetraethoxysilane teos, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Meilun (1 mentions)
Penicillin, by Meilun (1 mentions)
7 protocols using annexin 5 fitc apoptosis detection kit
1
Nanoparticle-Mediated Cancer Therapy
2
Cytotoxicity and Apoptosis Assessment
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High-purity (≧98%) CT, Cell counting Kit-8 (CCK8), and the Annexin V-FITC Apoptosis Detection Kit were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). DMEM medium (high glucose) was purchased from Hyclone Company (Hyclone, Logan, UT, USA). Fetal bovine serum (FBS) was obtained from Gibco (Grand Island, NY, USA). The BCA Protein Assay Kit and propidium iodide were purchased from Shanghai Yeasen Biotechnology Co., Ltd. (Shanghai, China). RNaseA was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The ATP Assay Kit was purchased from Shanghai Biyuntian Biotechnology Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO) was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). Myristic acid-1,2-13C2, N, O-Bis (trimethylsilyl) trifluoroacetamide (BSTFA), trimethylchlorosilane (TMCS), pyridine, and methoxamine were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Annexin V-FITC Apoptosis Assay Protocol
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The Annexin V-FITC Apoptosis Detection Kit (Meilunbio) was used to evaluate cell death in the hBMECs. For 1 h in the dark at room temperature, the transfected cells were incubated in 200 µL binding buffer containing 10 µL Annexin V-FITC and 5 µL propidium iodide (PI). Flow cytometry was then used to determine apoptosis. The results of the flow analysis were processed using the Flowjo software.
4
Annexin V-FITC Apoptosis Assay
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Apoptotic cells were determined by flow cytometry (Beckman CytoFlex S) after the cells were doubled stained with Annexin V and propidium iodide (PI). The Annexin V-FITC Apoptosis Detection Kit (Meilunbio) was used for the apoptosis assay as per the manufacturer’s protocols.
5
Investigating Bleomycin-Induced Oxidative Stress
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Bleomycin was purchased from Hanhui Pharma Co., Ltd. (Hangzhou, China). 4% paraformaldehyde was purchased from Wuhan Saiweier Biological Technology Co., Ltd (Wuhan, China). Hydroxyproline assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). CoQ10 (purity > 98%, using HPLC) was purchased from Sigma-Aldrich (St. Louis, MO, USA), dissolved in DMSO (St. Louis, MO, USA), and diluted into the culture medium. The final concentration of the DMSO was 0.1%. Annexin V-FITC apoptosis detection kit and total ROS/superoxide detection kit were purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China).
6
Fabrication and Evaluation of CTAC-based Nanoparticles
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Hexadecyl trimethyl ammonium chloride (CTAC, >99%) was obtained from Sinopharm Group Chemical Reagent Co., LTD (Shenyang, China). Triethanolamine (TEA, >95%) was purchased from Tianjin Bodi Chemical Co., Ltd (Tianjin, China). Tetraethoxysilane (TEOS, 98%) was purchased from Sigma-Aldrich (Shanghai, China). ICG, 1,3-diphenyl isobenzofuran (DPBF), membrane protein extraction kit, Annexin V-FITC apoptosis detection kit, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT), cholecystokinin octapeptide (CCK-8) assay kit, Dulbecco's Modified Eagle's Medium (DMEM), RPMI 1640, PBS (pH 7.4), fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin were all purchased from Meilun Biotechnology Co., Ltd (Dalian, China). Nitric oxide (NO) assay was acquired from Solarbio Science & Technology Co., Ltd. (Beijing, China). The mouse interleukin 12 (IL-12), mouse tumor necrosis factor-alpha (TNF-α) uncoated enzyme-linked immunosorbent assay (ELISA) kits, FITC-labeled F4/80, PE-labeled CD206 and APC-labeled CD86 monoclonal antibody were purchased from Thermo Fisher Scientific.
7
Apoptosis Analysis of PRV-Infected Cells
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Cell apoptosis was analyzed with an Annexin V-FITC apoptosis detection kit (Meilun Biotechnology Co., Ltd., Dalian, China) by flow cytometry. Briefly, PK15 cells infected with PRV were treated with emodin for 36 h. The cell culture medium was collected, added to digested adherent cells, and centrifuged at 1000× g for 5 min. After the cell pellet was washed with PBS and resuspended by adding 500 μL binding solution, 5 μL annexin V-FITC and 5 μL PI were added for 10 min in the dark at RT. Cell apoptosis was measured by flow cytometry.
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