Human prostate adenocarcinoma PC-3 cells (KCLB no. 21435), human prostate adenocarcinoma DU145 cells (KCLB no. 30081), and human fibrosarcoma HT1080 cells (KCLB no. 10121) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were maintained in RPMI 1640 or DMEM (Cellgro, Manassas, VA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Cellgro) and penicillin (100 U/mL)/streptomycin (100 μg/mL) (Cellgro) at 37°C in a humidified 5% CO2 incubator.
Pc 3 cells
Manufactured by Korean Cell Line Bank
Sourced in United States
PC-3 cells are a well-characterized human prostate cancer cell line derived from a bone metastasis. They are widely used in cancer research to study the biology and treatment of prostate cancer.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Corning (3 mentions)
Streptomycin, by Corning (3 mentions)
Rpmi 1640, by Corning (3 mentions)
Heat inactivated fetal bovine serum, by Corning (2 mentions)
Du145 cells, by Korean Cell Line Bank (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Endothelial cell growth medium 2 egm2, by PromoCell (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Ht1080 cells, by Korean Cell Line Bank (1 mentions)
B16f10 cells, by Korean Cell Line Bank (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
4 protocols using pc 3 cells
1
Cell Culture of Prostate and Fibrosarcoma
2
Cell Culture Protocols for Various Cell Lines
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HT1080 cells (KCLB No. 10121, human fibrosarcoma), PC-3 cells (KCLB No. 21435, human prostate adenocarcinoma), and B16F10 cells (KCLB No. 80008, murine melanoma) were obtained from Korean Cell Line Bank (Seoul, Korea) in 2012, where they were characterized by DNA fingerprinting. Cells were maintained in RPMI1640 or Dulbecco's modified Eagle's medium (DMEM, Cellgro, Manassas, VA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Cellgro) and 100 U/ml penicillin/100 μg/ml streptomycin (Cellgro) at 37°C in a humidified 5% CO2 incubator. Human umbilical vein endothelial cells (HUVECs) purchased from Innopharmascreen (Asan, Korea) in October 2012 were maintained in Endothelial cell Growth Medium-2 (EGM-2; PromoCell, Heidelberg, Germany) and used at passages 3 to 8. Cells were routinely screened for Mycoplasma contamination.
3
Cell Culture and Murine Hepatocyte Isolation
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Human fibrosarcoma HT1080 cells (KCLB no. 10121), human prostate adenocarcinoma PC-3 cells (KCLB no. 21435), and human gastric carcinoma AGS cells (KCLB no. 21739) were obtained from the Korean Cell Line Bank (Seoul, Korea) and cultured in RPMI 1640 (Cellgro, Manassas, VA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Cellgro) and penicillin (100 U/mL)/streptomycin (100 μg/mL) (Cellgro) at 37°C in a humidified 5% CO2 incubator. Murine hepatocytes were isolated using a perfusion system with some modification and incubated as described previously [19 (link)].
4
Stable Cell Lines for Fluorescence Assays
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Fisher rat thyroid (FRT) cells stably expressing ANO1 were provided by Alan Verkman (University of California, San Francisco, CA, USA). ANO1-expressing FRT cells were stably transfected with the halide sensor YFP-H148Q/I152L/F46L as described in a previous study [56 (link)]. FRT cells were cultured in Ham’s F-12 Modified medium with 10% fetal bovine serum (FBS), 100 units/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine. PC-3 cells were purchased from Korean Cell Line Bank (KCLB) and cultured in RPMI 1640 medium supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin. FRT cells and PC-3 cells were grown at 37 °C, 5% CO2, and 95% humidity. HEK293T cells were purchased from the American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C with 10% CO2.
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