Lungs were removed and fixed in 10% neutral-buffered formalin with 2 changes of formalin for a minimum of 7 days prior to processing. Lung tissue was processed with a Sakura Tissue-Tek VIP-5 processor on a 12-hour automated schedule using a graded series of ethanol, xylene, and Paraplast Xtra (MilliporeSigma). Paraffin-embedded tissues were sectioned at 5 μm thickness and dried overnight at 42°C before staining. Fixed tissue sections were stained with H&E or for SARS-CoV-2 N antigen using GenScript U864YFA140-4/CB2093 NP-1 diluted 1:1000 with an anti-rabbit IgG polymer (Vector Laboratories ImPress VR). Tissues were processed for IHC using a Discovery Ultra automated processor (Ventana Medical Systems) with ChromoMap DAB Kit (Roche Tissue Diagnostics). Stained slides were examined on an Olympus BX51 light microscope equipped with an Olympus DP722 camera and associated cellSens Dimension 1.4.1 software. Pathological analysis was performed blinded by a board-certified pathologist. Lesions were scored from 0 (no lesions) to 5 (severe).
Impress vr
Manufactured by Vector Laboratories
The ImPress VR is a versatile lab equipment product from Vector Laboratories. It is designed for high-quality image capture and processing, with features tailored for virtual reality applications. The product's core function is to facilitate the creation and manipulation of VR content.
Automatically generated - may contain errors
Lab products found in correlation
Chromomap dab kit, by Roche (5 mentions)
Discovery ultra, by Roche (3 mentions)
Bx51 light microscope, by Olympus (1 mentions)
U864yfa140 4 cb2093 np 1, by Vector Laboratories (1 mentions)
U864yfa140 4 cb2093 np 1, by GenScript (1 mentions)
Discovery ultra automated processor, by Roche (1 mentions)
Bx53 light microscope, by Olympus (1 mentions)
5 protocols using impress vr
1
SARS-CoV-2 Lung Tissue Analysis Protocol
2
SARS-CoV-2 Tissue IHC Staining
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Tissues samples were collected, placed into cassettes and fixed in 10% formalin for 7 days. Prior to removal from BSL-4, tissues were transferred into fresh 10% formalin and incubated an additional 24 hours. Tissues were then processed with a Sakura VIP-6 Tissue Tek, on a 12-hour automated schedule, using a graded series of ethanol, xylene, and PureAffin. Following processing, samples were embedded in Pureaffin paraffin polymer (Cancer Diagnostics, Durham, NC, USA) and sectioned at 5 μm and dried overnight at 42°C prior to hematoxylin and eosin (H&E) staining. IHC tissues were processed using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) for immunohistochemistry (IHC) staining. Specific immunoreactivity was detected using a validated GenScript U864YFA140–4/CB2093 NP-1 SARS-CoV-2-specific antiserum at a 1:1000 dilution. The secondary antibody was an anti-rabbit IgG polymer (cat# MP-6401) from Vector Laboratories ImPress VR as previously described [21 (link)].
3
Immunohistochemical Detection of SARS-CoV-2 Nucleoprotein
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Tissues were fixed in 10% neutral buffered formalin (with two changes) for a minimum of 7 days. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek on a 12-hour automated schedule, using a graded series of ethanol, xylene, and ParaPlast Extra. Embedded tissues are sectioned at 5um and dried overnight at 42°C prior to staining. Specific anti-CoV immunoreactivity was detected using GenScript U864YFA140–4/CB2093 NP-1 at a 1:1,000 dilution. The secondary antibody is an anti-rabbit IgG polymer from Vector Laboratories ImPress VR. Tissues were then processed for immunohistochemistry using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics).
4
Histopathological and Immunohistochemical Analysis of SARS-CoV-2 in Rhesus Macaques
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Histopathology and IHC were performed on rhesus macaque tissues. After fixation for a minimum of 7 days in 10% neutral-buffered formalin and embedding in paraffin, tissue sections were stained with H&E. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek on a 12-hour automated schedule, using a graded series of ethanol, xylene, and ParaPlast Extra. Embedded tissues were sectioned at 5 μm and dried overnight at 42°C before staining. Specific anti-CoV immunoreactivity was detected using GenScript U864YFA140-4/CB2093 NP-1 at a 1:1000 dilution. The secondary antibody was an anti–rabbit IgG polymer from Vector Laboratories ImPress VR. Tissues were then processed for IHC using the Discovery Ultra automated processor (Ventana Medical Systems) with a ChromoMap DAB Kit (Roche Tissue Diagnostics). Stained slides were analyzed by a board-certified veterinary pathologist.
5
Histopathological Analysis of Organ Tissues
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