Anti snail slug antibody

Patient stomach tissues were collected at the First Affiliated Hospital of Bengbu Medical College after obtaining informed consent and institutional approval. The anti-MAL antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The rabbit anti-CD44 monoclonal antibody, anti-STAT3 (phospho-Y705) antibody, anti-snail + slug antibody, anti-vimentin antibody, anti-E-cadherin antibody, anti-STAT3 antibody, anti-GAPDH antibody, anti-alpha SMA antibody, horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (H + L) antibody, HRP-conjugated goat anti-rabbit IgG (H + L) antibody, Alexa Fluor^® 594-conjugated goat anti-mouse IgG (H + L) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG (H + L) antibody were purchased from Abcam (Cambridge, MA, United States).

Histological and immunohistochemical sections were prepared [27 (link)] and stained with hematoxylin and eosin (HE) and Masson’s trichrome (MT) according to standard protocols. For IHC, antigens were activated using a Histofine simple stain kit (Nichirei Biosciences, Tokyo, Japan), and the sections were stained with anti-COL1A1 antibody (Cell Signaling Technology, 72026), anti-α-SMA antibody (Cell Signaling Technology, 19245), anti-ZEB1 antibody (Cell Signaling Technology, 70512), anti-SNAIL + SLUG antibody (Abcam, ab180714), and anti-Lamin B1 antibody (Synaptic Systems GmbH, Göttingen, Germany, HS-404013). TUNEL staining was performed using the In Situ Cell Death Detection Kit (Sigma Aldrich, 1684795).