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2 protocols using kt109

1

Comprehensive Cannabinoid Receptor Characterization

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Chemicals were of the purest analytical grade. MEM, glutamine, PES, DMSO, collagenase, DNase1, trypsin, BPA (Bisphenol A), ABTS (2,2′-azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt), and hyaluronidase were purchased from Sigma (St. Louis, MO, USA). Rabbit anti-CB1, anti-CB2, anti-GPR55, anti-FAAH, anti-DAGL-α, anti-DAGL-β, anti-MAGL, anti-NAPE-PLD polyclonal antibodies, and KT109 were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Mouse anti-actin antibody was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and rabbit anti-TRPV1 polyclonal antibody was from OriGene Technologies (Rockville, MD, USA). Rabbit anti-inhibin B polyclonal antibody was obtained from Elabscience Biotechnology (Houston, TX, USA), and rabbit anti-transferrin polyclonal antibody was from Proteintech Group (Chicago, IL, USA). Iodoresinferatoxin was obtained from Tocris Cookson (Bristol, UK), whereas SR144528 was a kind gift from Sanofi-Synthelabo (Montpellier, France).
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2

Macrophage Lipid Characterization Protocol

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Unless otherwise specified, reagents used were purchased from Fisher Scientific. Bio-beads SM-2 Adsorbants (Bio-Rad) catalog# 1528920. Polyethyleneimine (Polysciences, Inc.) catalog# 24765, 2-Mercaptoethanol (Sigma-Aldrich) catalog# M6250–250ML, recombinant M-CSF (Peprotech) catalog# 315–02. KT109 (synthesized in house), KT195 (Cayman Chemical Company) catalog# 14818. Anti-FLAG antibody produced in rabbit (Sigma-Aldrich) catalog# F7425, Goat anti-rabbit DyLight 550 (Thermo Fisher Scientific) catalog# 84541. PE/Cy7 anti-mouse/human CD11b antibody (Biolegend) catalog# 101215. Rat anti-mouse F4/80 antibody (Bio-Rad) catalog# MCA497A488T. Arachidonic acid-d8 (Cayman Chemical Company) catalog# 390010, 2-Arachidonyl glycerol-d5 (Cayman Chemical Company) catalog# 362162, Tritetradecanoin (C14:0) (Nu-Chek Prep) catalog# T-140, Trihexadecanoin (C16:0) (Nu-Chek Prep) catalog# T-150, Trioctadecanoin (C18:0) (Nu-Chek Prep) catalog# T-160, Trieicosanoin (C20:0) (Nu-Chek Prep) catalog# T-170, Tridocosanoin (C22:0) (Nu-Chek Prep) catalog# T-180, Triarachidonin (C20:4) (Nu-Chek Prep) catalog# T-295, Trieicosapentaenoin (C20:5) (Nu-Chek Prep) catalog# T-325, Tridocosahexaenoin (C22:6) (Nu-Chek Prep) catalog# T-310. Fetal bovine serum (FBS) and dialyzed FBS were obtained from Omega Scientific.
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