Log In Sign up
The largest database of trusted experimental protocols

Goat anti rabbit hrp 12 348

Manufactured by Merck Group
Visit Supplier
Sourced in United Kingdom

Goat anti-rabbit HRP (12–348) is a secondary antibody reagent that binds to rabbit primary antibodies. It is conjugated with horseradish peroxidase (HRP), which can be used to detect and visualize the bound primary antibodies in various immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Ab54481, by Abcam (2 mentions) Goat anti mouse hrp 170 6516, by Bio-Rad (2 mentions) Ab174833, by Abcam (1 mentions) Ab19481, by Abcam (1 mentions) Ab19534, by Abcam (1 mentions) Ab3508, by Abcam (1 mentions) Ab16801, by Abcam (1 mentions) Ab124811, by Abcam (1 mentions) Ab126704, by Abcam (1 mentions) Ab53179, by Abcam (1 mentions) Ab37185, by Abcam (1 mentions) Ab49900, by Abcam (1 mentions) Anti glut4 g4048, by Merck Group (1 mentions) Pierce protein a g agarose, by Thermo Fisher Scientific (1 mentions) Ab24509, by Abcam (1 mentions) Ab68155, by Abcam (1 mentions) Ab189860, by Abcam (1 mentions) Ab190479, by Abcam (1 mentions) Ab181602, by Abcam (1 mentions) Chemidoctm touch imaging system, by Bio-Rad (1 mentions)

3 protocols using goat anti rabbit hrp 12 348

1

Protein Expression Analysis in Metabolic Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals and reagents used in the study, which were of molecular and analytical grade, were purchased from Sigma Chemical Co. (St. Louis, MO, USA), unless otherwise mentioned. Antibodies against FNDC5 (ab174833), and PGC-1α (ab54481) were purchased from Abcam. The anti-CTH (WH0001491M), anti-GLUT4 (G4048), and anti-GAPDH HRP (G9295) antibodies were purchased from Sigma Aldrich. Goat anti-mouse HRP (170–6516) was purchased from Biorad and the goat anti-rabbit HRP (12–348) from Millipore.
Parsanathan R, & Jain S.K. (2022). Hydrogen Sulfide Regulates Irisin and Glucose Metabolism in Myotubes and Muscle of HFD-Fed Diabetic Mice. Antioxidants, 11(7), 1369.
+ Open protocol
+ Expand
2

Comprehensive Glutathione Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals, unless specified, were purchased from Sigma. Antibodies against glutathione [D8] (ab19534), GCLC (ab53179), GCLM [EPR6667] (ab126704), glutathione synthetase [EPR6562] (ab124811), glutathione reductase (ab16801), xCT or Slc7a11 (ab37185), PPARα (ab24509), PPARγ (ab19481), PGC1α (ab54481), Vitamin D receptor (ab3508), and β-actin HRP (ab49900) were purchased from Abcam. The anti-CTH (WH0001491M) and anti-GLUT4 (G4048) were purchased from Sigma Aldrich. Goat anti- mouse HRP (170–6516) was purchased from Biorad and the goat anti-rabbit HRP (12–348) from Millipore. Pierce™ protein A/G agarose was purchased from Thermo Scientific.
Parsanathan R, & Jain S.K. (2018). Hydrogen sulfide increases glutathione biosynthesis, and glucose uptake and utilization in C2C12 mouse myotubes. Free radical research, 52(2), 288-303.
+ Open protocol
+ Expand
3

Hippocampal Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was extracted from hippocampal tissues with protein lysis buffer (Beyotime Biotech, China). The protein concentration was determined with a BCA kit (P0010S, Beyotime Biotech). The proteins in each sample were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidene uoride (PVDF) membranes. Western blot analysis was carried out using the following primary antibodies raised against target proteins: rabbit anti-acetyllysine (dilution 1:1000, ab190479, Abcam, UK), rabbit anti-Sirt3 (dilution 1:1000, ab189860, Abcam), rabbit anti-SOD2 (dilution 1:1000, ab68155, Abcam), rabbit anti-Acetyl-SOD2(dilution 1:1000, AF3751, A nity Biosciences, China) and rabbit anti-β-actin (dilution 1:1000, ab181602, Abcam) antibodies overnight at 4 °C. The following secondary horseradish peroxidase (HRP)-conjugated antibodies were used at 1: 5000 dilution: goat antirabbit HRP (12-348) (Millipore, Watford, UK); and goat anti-mouse (sc-2005) (Santa Cruz, Insight Biotechnology, Wembley, UK). The blots were visualized with enhanced chemiluminescence detection reagents using a Chemidoc TM Touch Imaging System (Bio-Rad).
Wang G., Hao K., Wang H., Zhang Y., Xie X., Huang H., Chen C., Xu S., Xu R., Shu C., Liu Z., Zhou Y., & Reynolds G.P. (2022). Nicotinamide Reverses Deficits in Puberty-born Neurons and Cognitive Function after Maternal Separation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!