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11 protocols using zn no3 2

1

Mung Bean Seed Heavy Metal Uptake

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MA, urea, K2Cr2O7, ascorbic acid, NaOH and HCl were purchased from Aladdin Chemical Co., Ltd (Shanghai, China). Other chemicals, including NaCl, KCl, AgNO3, CuCl2·2H2O, CaCl2·2H2O, FeCl2, MnSO4·H2O, BaCl2, NiCl2·6H2O, MgCl2, Zn(NO3)2, FeCl3·6H2O, Al(NO3)3·9H2O and CrCl3·6H2O, were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Mung bean seeds were purchased from retail outlets. All the chemical agents were of analytical grade and used as received without further purification. Deionized water was used for preparing the solutions.
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2

Electrolyte Composition Effects on Zinc-Based Energy Storage

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The ZS electrolyte (2 m ZnSO4) was prepared by dissolving 17.25 g of ZnSO4 (99.995%, Aladdin) into 30 ml of deionized (DI) water. The La3+-ZS electrolyte (2 m ZnSO4 and 0.0085 m La(NO3)3) was prepared by adding 110.4 mg of La(NO3)3 (99%, Aladdin) into 30 mL of ZS solution. To inquire the role of NO3, NO3-ZS electrolyte with a same concentration of NO3 as La3+-ZS was prepared by adding 113.8 mg of Zn(NO3)2 (AR, Sinopharm Chemical) into 30 mL of ZS solution.
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3

Synthesis of ZnO Nanostructures

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As shown in Figure 1, the seed layer coated substrates were first immersed in Zn(NO3)2 (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China)/hexamethylenetetramine (HMTA) (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China)/DAP (Macklin Inc., Shanghai, China) (0.05 M Zn(NO3)2, Zn(NO3)2, and DAP in the proportion 1:6) aqueous solution (10 min) and then was heated in a constant temperature water bath (90 °C, 3 h). After the reaction, the substrates were separated from the solution and washed with ultra-pure water to obtain the substrates with ZnO nanostructures.
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4

Biochar-Based Heavy Metal Removal

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Chemical reagents of an analytic grade, including cystamine dihydrochloride, glutaraldehyde, methanol, anhydrous ethanol, NaOH, HNO3, HCl, Cd(NO3)2, Zn(NO3)2, AS(NO3)2, Ni(NO3)2, Cr(NO3)2, and Co(NO3)2 were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All solutions were prepared using deionized (DI) water. The biochar, which was pyrolyzed by rice husk at 400–450 °C, was from Liao Ning Golden Future Agriculture Technology Co., Ltd. (Anshan, Liaoning Province, China).
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5

Microcantilever-based Pb2+ Detection

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We used commercially available silicon microcantilevers (Mikro Masch Co., Sunnyvale, CA) in all of our experiments. The dimensions were 350 μm in length, 35 μm in width, and 1 μm in thickness. One side of the cantilever was covered with a thin film of chromium (20 nm) followed by a 20-nm layer of gold, and the other side was silicon with a thin naturally grown oxide layer.
All the chemicals were of analytical grade. The chemicals including Pb(NO3)2, Mg(NO3)2, Fe(NO3)3, Cd(NO3)2, Zn(NO3)2, and Ca(NO3)2 were obtained from Sinopharm Chemical Reagent Beijing Co., Ltd. To tether the oligonucleotide to the gold surface of microcantilevers by Au-S bond, the 3′ end was tailored with an alkanethiol linker X (5′-GGGTGG-GTGGGTGGGT-3′-(CH2)6SH), 20 the thiolated G-rich singlestranded oligonucleotide Pb 2+ -dependent DNAzyme X and a single-stranded oligonucleotide Y (3′-C-ACCCTCCCAC-5′, which is partially complementary to the DNAzyme X); these were obtained from Sangon Biotechnology Co., Ltd. (Shanghai, PRC). Also, 3-(N-morpholino)propanesulfonic acid (MOPS) and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) were purchased from Sigma-Aldrich. All reagents were used as received without further purification. The stock solution of oligonucleotide (100 μM) was prepared in 10 mM Tris-HAc buffer (pH 8.0).
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6

Ornithine-Based Bioactive Compound Analysis

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l-Ornithine hydrochloride was obtained from Sigma-Aldrich Co. Ltd (Shanghai, China). FeCl3, NaCl, KCl, CaCl2, CuCl2, MgCl2, FeCl2, Zn(NO3)2, BaCl2, Pb(NO3)2, AlCl3, NiSO4, CoCl2, Cr2(SO3)3, CdSO4, Hg(NO3)2, MnCl2, MTT, Dulbecco's modified Eagle medium (DMEM) and quinine sulfate were purchased from Sinopharm Chemical Reagent Co. Ltd. RPMI-1640 medium was obtained from Gibco Company Ltd (USA). Fetal bovine serum was purchased from Hyclone Company Ltd (USA). A549 cells were purchased from the American Type Culture Collection (ATCC). Sephadex G-25 was purchased from GE Healthcare. All other chemical reagents were of analytical grade and used without any further purification. Ultrapure water (18.2 MΩ cm) was prepared using a Milli-Q system.
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7

Kaolinite-Based Heavy Metal Adsorption

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The kaolinite was purchased from Chengdu Chenzheng Chemical Co., Ltd. Its X-ray fluorescence results showed that, the main components of kaolinite are SiO2 (55.2%), Al2O3 (38.74%) and K2O(3.27%). Zn(NO3)2, Pb(NO3)2, and Cu(NO3)2 were purchased from Sinopharm Chemical Reagent Co. Ltd. They were of analytical grade and were used without further purification. Deionized water was used for the preparation of all solutions. The pH adjustment of solutions was performed using 0.1 mol/L HNO3 or 0.1 mol/L NaOH.
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8

Zinc-Ion Solid-State Battery Assembly

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The reagents [e.g. Zn plate, Cu plate, Zn(NO3)2 and LiNO3] were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai). Zinc plate (0.2 mm in thickness, 99.9%) and copper plate (0.1 mm in thickness, 99.9%) were polished before battery assembly. The 2.5 × 2.5 cm2 ceramic lithium super-ionic conductor film (LATSP, Li1+x+yAlxTi2-xSiyP3-yO12 purchased from Ohara Inc., Japan) with a thickness of 0.15 mm and a Li-ion conductivity of 10−4 S cm−1 was used as received. The glass microfiber filter was obtained from Whatman® Anodisc Inorganic Membranes. 2 M LiNO3 solution and 1 M Zn(NO3)2 solution were used as cathodic electrolyte and anodic electrolyte, respectively. Detailed information about the battery assembly was given in Figure S4.
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9

Synthesis and Characterization of H4TCPB

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1,2,4,5-Tetrakis (4-carboxyphenyl) benzene (H4TCPB, 98%) was supplied by Jilin Chinese Academy of Science-Yanshen Technology Co., Ltd (Changchun, China). Hydrochloric acid (HCl, 36-38%) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai China) N, N-dimethylformamide (DMF, 99.5%), ethanol (99.7%), methanol (99.5%), acetone (99.5%), tetrahydrofuran (99.5%) nitric acid (HNO3, 65%), NaNO3 (99%), KNO3 (99%), Ca(NO3)2 (99%), Ti(NO3)4 (99%), Zn(NO3)2 (99%), Co(NO3)2, (99%), and Fe(NO3)3 (99%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai China) A wide range of antibiotics and chemicals, i.e., tetracycline, penicillin sodium, salicylic acid, diuron, disulfiram, and norfloxacin, were purchased from Shanghai Maclin Biochemical Technology Co., Ltd. (Shanghai China).
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10

Synthesis and Characterization of H4TCPB

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1,2,4,5-Tetrakis (4-carboxyphenyl) benzene (H4TCPB, 98%) was supplied by Jilin Chinese Academy of Science-Yanshen Technology Co., Ltd (Changchun, China). Hydrochloric acid (HCl, 36-38%) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai China) N, N-dimethylformamide (DMF, 99.5%), ethanol (99.7%), methanol (99.5%), acetone (99.5%), tetrahydrofuran (99.5%) nitric acid (HNO3, 65%), NaNO3 (99%), KNO3 (99%), Ca(NO3)2 (99%), Ti(NO3)4 (99%), Zn(NO3)2 (99%), Co(NO3)2, (99%), and Fe(NO3)3 (99%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai China) A wide range of antibiotics and chemicals, i.e., tetracycline, penicillin sodium, salicylic acid, diuron, disulfiram, and norfloxacin, were purchased from Shanghai Maclin Biochemical Technology Co., Ltd. (Shanghai China).
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