Human pancreatic cancer cell lines MiaPaCa-2, BxPC-3, PANC-1, and Capan-1 were purchased from the European Collection of Cell Cultures (Salisbury, UK). Cells were maintained in DMEM (MiaPaCa-2) and RPMI1640 (BxPC-3, PANC-1 and Capan-1) supplemented with 10% FBS, 100 unit/mL penicillin G and 0.1 mg/mL streptomycin sulfate. Five-week-old male nude mice were purchased from Charles River Japan (Yokohama, Japan), which were housed in specific pathogen-free conditions. The experimental protocols were performed in accordance with the Care and Use of Laboratory Animals of the University of Tokushima School of Medicine and were approved by the Animal Care and Use Committee. Recombinant human IGF-1 was purchased from PeproTech (Rocky Hill, NJ) and human HB-EGF and IGF-2 were purchased from R&D systems (Minneapolis, MN). The EP2-selective antagonist AH6809 and EP4-selective antagonist GW627368X were purchased from Cayman Chemical (Ann Arbor, MI). The pseudo-substrate of PKC-θ was purchased from Merck Millipore (Billerica, MA).
Mia paca 2
Manufactured by European Collection of Cell Cultures
Sourced in United Kingdom
MIA PaCa-2 is a human pancreatic carcinoma cell line. It is used for in vitro cell culture studies.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Panc 1, by European Collection of Cell Cultures (3 mentions)
Capan 1, by European Collection of Cell Cultures (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Gw627368x, by Cayman Chemical (1 mentions)
Human hb egf, by R&D Systems (1 mentions)
Recombinant human igf 1, by Thermo Fisher Scientific (1 mentions)
Ah6809, by Cayman Chemical (1 mentions)
Gemcitabine hydrochloride, by Bio-Techne (1 mentions)
Azd6738, by AstraZeneca (1 mentions)
Yoyo 3, by Thermo Fisher Scientific (1 mentions)
Mycoprobe mycoplasma detection kit, by R&D Systems (1 mentions)
Geneprint 10 system, by Promega (1 mentions)
Mia paca 2, by Promega (1 mentions)
Mia paca 2, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
Wi 38, by Merck Group (1 mentions)
M2279, by Merck Group (1 mentions)
8 protocols using mia paca 2
1
Human Pancreatic Cancer Cell Lines Characterization
2
Comprehensive Pancreatic Cancer Cell Line Protocol
3
Combination therapy for pancreatic cancer
4
Lectins Effect on Cell Growth
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The effect of lectins on cell growth was determined in a primary human umbilical vein endothelial cells (HUVECs), a mouse fibroblast cell line (L929; Passage No. 40), and in a panel of human tumor cells including lung adenocarcinoma (A549; Passage No. 37), acute monocytic leukemia cell line (THP-1; Passage No. 16) and pancreatic adenocarcinoma (PANC-1; Passage No. 29), Human pancreatic ductal adenocarcinoma cell line (CFPAC-1; Passage No.25), Human pancreatic epithelial carcinoma cell line (MIA PaCa-2; Passage No.19) and cervix adenocarcinoma (HeLa) obtained from the European Collection of Cell Cultures (ECCC, Salisbury, UK). HUVECs were maintained in M200 Media supplemented with 50X LVES (Gibco, Invitrogen); THP-1 was maintained in RPMI 1640; L929, A-549, PANC-1, CFPAC-1 and MIA PaCa-2 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM). HeLa and macrophages were cultured in Eagle's Minimum Essential Medium (EMEM). All media used were supplemented with 10% fetal bovine serum (FBS; Gibco) and the cells were maintained at 37°C and 5% CO2 in a humidified atmosphere.
5
Cell Line Maintenance and Authentication
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C26 (murine colon adenocarcinoma 26 cell line) and Miapaca2 (human pancreas cancer cell line) were obtained from a cell line collection of Otsuka Pharmaceutical Co., Ltd. Caki2 (human Caucasian kidney carcinoma cell line) was purchased from the European Collection of Cell Cultures. T24 (human bladder cancer cell line) was supplied by the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer at Tohoku University. Miapaca2 and T24 were genetically authenticated using STR analysis (Promega, October 2012). C26 cells were maintained in RPMI1640, T24 and Caki2 cells in McCoy's 5A, and Miapaca2 cells in D-MEM/Ham's F-12 with 10% fetal bovine serum (FBS), 50 U/mL of penicillin, and 50 µg/mL of streptomycin (a penicillin/streptomycin mixture) in a humidified atmosphere containing 5% CO2 at 37°C.
6
Culturing Human Pancreatic Cancer Cells
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Human pancreatic cancer cell lines PANC-1 and MiaPaca-2 were obtained from European Collection of Cell Cultures (ECACC, Wiltshire, UK) and cultured in Dulbecco's modified Eagle's medium (GIBCO, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific) and 1% penicillin/streptomycin (GIBCO, Thermo Fisher Scientific). Cells were incubated at 37°C and 5% CO2 in a humidified chamber.
7
Culturing Pancreatic Cancer Cell Lines
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The human pancreatic cell-line MIAPaCa-2 was obtained from the European Collection of Cell Cultures (ECACC, UK). BxPC-3 and PANC-1 human pancreatic cancer cell lines were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). PIN-127 cells were derived in-house from a patient-derived xenograft model as previously described (21) (link).
PANC-1 and MIAPaCa-2 cells were grown in DMEM (Sigma-Aldrich, Dublin, Ireland) supplemented with 5% (v/v) fetal bovine serum (FBS) (Thermo Fischer Scientific, UK) and 2% L-glutamine. BxPC-3 cells were grown in RPMI-1640 medium supplemented with 5% (v/v) FBS. PIN-127 cells were cultured in DMEM/F-12 Hams medium supplemented with 10% (v/v) FBS (Corning, SA). Cells were grown in a humidified atmosphere with 5% CO 2 at 37℃ in culture flasks. Cells were sub-cultured, using PBS to wash and trypsin-EDTA (Bio-Sciences Ltd, Ireland) to detach when cells reached 80-90% confluency.
PANC-1 and MIAPaCa-2 cells were grown in DMEM (Sigma-Aldrich, Dublin, Ireland) supplemented with 5% (v/v) fetal bovine serum (FBS) (Thermo Fischer Scientific, UK) and 2% L-glutamine. BxPC-3 cells were grown in RPMI-1640 medium supplemented with 5% (v/v) FBS. PIN-127 cells were cultured in DMEM/F-12 Hams medium supplemented with 10% (v/v) FBS (Corning, SA). Cells were grown in a humidified atmosphere with 5% CO 2 at 37℃ in culture flasks. Cells were sub-cultured, using PBS to wash and trypsin-EDTA (Bio-Sciences Ltd, Ireland) to detach when cells reached 80-90% confluency.
8
Cell Line Maintenance and Passage
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The cell lines MCF7, A549, ALT, PANC-1, MIA-PaCa-2 (European Collection of Cell Cultures) and WI38 (American Type Culture Collection) were maintained in monolayer culture in 75 cm2 flasks (TPP, Switzerland) under a humidified 5 % CO2 atmosphere at 37°C. For the A549 cell line, Dulbecco's MEM medium (GIBCO 21969, Invitrogen, UK) supplemented with L-glutamine (2 mM, GIBCO 25030, Invitrogen, UK), essential amino acids (1 %, GIBCO 11140, Invitrogen, UK), and foetal calf serum (10 %, S1810, Biosera, UK) was used. For MIA-Pa-Ca-2 and PANC-1, Dulbecco's MEM, supplemented with L-glutamine (2 mM) and foetal calf serum (10 %) was used.
The medium MEM (M2279, Sigma, UK) with added L-glutamine (2 mM), essential amino acids (1 %) and foetal calf serum (10 %) was used for the MCF7, ALT and WI38 cell lines. To passage the cells, they were washed with PBS (GIBCO 14040, Invitrogen, UK), treated with trypsine (GIBCO 25300, Invitrogen, UK), and re-seeded into fresh medium, resulting in an initial cell density of approximately 1x10 4 cells/mL medium. Cells were counted using a Neubauer haemocytometer (Assistant, Germany) by microscopy or a MacsQuant flow cytometer (Miltenyi Biotech, Germany) on a suspension of cells obtained by washing with PBS, trypsinisation, centrifugation at 8 °C at 8000 rpm for 3 minutes, and re-suspension in fresh medium.
The medium MEM (M2279, Sigma, UK) with added L-glutamine (2 mM), essential amino acids (1 %) and foetal calf serum (10 %) was used for the MCF7, ALT and WI38 cell lines. To passage the cells, they were washed with PBS (GIBCO 14040, Invitrogen, UK), treated with trypsine (GIBCO 25300, Invitrogen, UK), and re-seeded into fresh medium, resulting in an initial cell density of approximately 1x10 4 cells/mL medium. Cells were counted using a Neubauer haemocytometer (Assistant, Germany) by microscopy or a MacsQuant flow cytometer (Miltenyi Biotech, Germany) on a suspension of cells obtained by washing with PBS, trypsinisation, centrifugation at 8 °C at 8000 rpm for 3 minutes, and re-suspension in fresh medium.
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