Anti gapdh

Manufactured by Synaptic Systems

Sourced in Germany

Anti-GAPDH is a primary antibody that specifically binds to the GAPDH protein. GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is a commonly used housekeeping gene and is involved in the glycolytic pathway. The Anti-GAPDH antibody can be used to detect and quantify GAPDH expression levels in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti vgat, by Synaptic Systems (1 mentions) Anti c myc, by Roche (1 mentions) Anti green fluorescent protein, by Takara Bio (1 mentions) Anti vglut1, by Synaptic Systems (1 mentions) Anti histone h3, by Cell Signaling Technology (1 mentions) Hrp conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti rabbit igg ap307p, by Merck Group (1 mentions) Hrp conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti app, by Abcam (1 mentions) C myc antibody, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 555 conjugated α bungarotoxin, by Thermo Fisher Scientific (1 mentions) Anti bace1 d10e5, by Cell Signaling Technology (1 mentions) Anti flag, by Merck Group (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti gapdh

Generating Polyclonal Anti-Shank3a Antibody

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A novel polyclonal antibody directed against the rat Shank3a N-terminus (aa 333-470) was generated for this study according to the antibody production and purification protocol described in [31 (link)]. The anti-Shank3 PRC antibody has been described previously [31 (link)]. The following primary antibodies were purchased from commercial suppliers: anti-histone H3 (Cell Signaling Technology, Danvers, MA, USA), anti-green fluorescent protein (GFP) (Clontech, Laboratories, Mountain View, CA, USA), anti-c-Myc (Roche Applied Science, Mannheim, Germany), as well as anti-GAPDH, anti-VGLUT1, and anti-VGAT (all from Synaptic Systems, Goettingen, Germany).

Cochoy D.M., Kolevzon A., Kajiwara Y., Schoen M., Pascual-Lucas M., Lurie S., Buxbaum J.D., Boeckers T.M, & Schmeisser M.J. (2015). Phenotypic and functional analysis of SHANK3 stop mutations identified in individuals with ASD and/or ID. Molecular Autism, 6, 23.

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Antibody-based Protein Detection Protocol

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The following antibodies and reagents were used: Alexa Fluor^® 555-conjugated α-bungarotoxin (B35451, Life Technologies), Hoechst 33342 (H3570, Life Technologies), anti-FLAG (F7425, Sigma-Aldrich), anti-SNX3 (sc-10619, Santa Cruz), anti-SNX3 (sc-376667, Santa Cruz), c-Myc antibody (9E10, Santa Cruz), anti-GAPDH (Synaptic System), anti-BACE1 (D10E5, Cell Signaling), anti-APP (22C11, Millipore), anti-APP (Y188, Epitomics), and the corresponding secondary antibodies HRP-conjugated goat anti-rabbit IgG (AP307P, Millipore), HRP-conjugated goat anti-mouse IgG (31430, Thermo Scientific), HRP-conjugated rabbit anti-goat IgG (Invitrogen), Alexa Fluor 555-conjugated goat anti-mouse IgG (A21424, Life Technologies).

Xu S., Nigam S.M, & Brodin L. (2018). Overexpression of SNX3 Decreases Amyloid-β Peptide Production by Reducing Internalization of Amyloid Precursor Protein. Neuro-degenerative diseases, 18(1), 26-37.

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