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Jem 1010 transmission electron microscope

Manufactured by Olympus
Sourced in Germany

The JEM-1010 is a transmission electron microscope manufactured by Olympus. It is designed to provide high-resolution imaging and analysis of samples at the nanoscale level. The core function of the JEM-1010 is to transmit a beam of electrons through a thin specimen, creating a magnified image that can be observed and analyzed.

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3 protocols using jem 1010 transmission electron microscope

1

Ultrastructural Analysis of LTX-315 Treated A375 Cells

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A375 cells were seeded at 1 × 105 cells per well in 6-well plates and allowed to grow for three days to optimize membrane structures in the culture, and the medium was changed on the second day. Cells were washed twice in serum-free RPMI before being treated with LTX-315 dissolved in serum-free RPMI at 3.5 and 17 μM, with serum-free RPMI as a negative control. Cells were then washed with PBS before fixation for 24 hours at 4°C with 4% formal aldehyde and 1% gluteralaldehyde in a Hepes buffer at pH 7.8. Dehydration and post-fixation protocols included incubation in a 5% buffered tannic acid and incubation in 1% osmium-reduced ferrocyanide. Ultrathin sections were prepared, and uranyl acetate (5%) and Reynolds's lead citrate were used for staining and contrasting. Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany).
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2

Ultrastructural Analysis of Peptide-Treated Cells

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Cells were seeded at 1 × 105 cells per well in 6-well plates and allowed to grow for three days to optimize membrane structures in the culture. Cells were then treated with peptides dissolved in serum-free RPMI at 25 μM. Cells were then washed with PBS before fixation for 24 h at 4 °C with 4% formal aldehyde and 1% glutaraldehyde in a HEPES buffer at pH 7.8. Dehydration and post-fixation protocols included incubation in a 5% buffered tannic acid and incubation in 1% osmium-reduced ferrocyanide. Ultrathin sections were prepared, and uranyl acetate (5%) and Reynolds’s lead citrate were used for staining and contrasting13 (link). Samples were examined on a JEOL JEM-1010 transmission electron microscope, and images were taken with an Olympus Morada side-mounted TEM CCD camera (Olympus soft imaging solutions, GmbH, Germany).
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3

Zebrafish Larvae Ultrastructure Analysis

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Before being used for electron microscopy the zebrafish larvae were anesthetized with 200 µg/ml tricaine, imaged alive by CLSM and afterwards immediately fixated in 2% glutaraldehyde and 2% paraformaldehyde in sodium cacodylate buffer (pH 7.2) for 3 h at room temperature followed by fixation for 16 h at 4 °C. Postfixation was performed in 1% osmium tetroxide in sodium cacodylate buffer for 1 h at room temperature. After dehydration through a graded series of ethanol all specimens were kept in epoxy resin (Agar Scientific, AGR1043) for 16 h before embedding. Ultrathin sections were collected on Formvar coated 200 mesh or one hole copper grids (Agar Scientific, AGS162) stained with 2% uranyl acetate in 50% ethanol and lead citrate for 10 min each. Electron microscopy images were obtained with a JEOL JEM-1010 transmission electron microscope (Tokyo, Japan) equipped with an Olympus Megaview camera (Tokyo, Japan).
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