Chemical identification was done in gas chromatography coupled to a mass spectrometer (GC-MS; GCQP-2010 Ultra, Shimadzu Corp., Kyoto, Japan), equipped with a non-polar stationary phase column (30 m × 25 μm × 25 mm; Rtx-1MS; RESTEK, Bellefonte, PA, USA), and helium as carrier gas (41.1 cm/s). One microliter of fungal and plant extracts was injected at 250 °C in splitless mode using GC oven program for VOCs quantification described above. Quadrupole ion source and transfer line were kept at 250 °C for electron impact analysis at 70 eV (35–270 m/z). Fungal and plant VOCs were tentatively identified based on a comparison of mass spectra with the library database (NIST11) and Kovats retention indices. When available, authentic standards were used to confirm the identification of compounds, which were: benzaldehyde, 1-octen-3-ol, 3-hexenol-acetate, 2-octen-1-ol, and phenethyl alcohol, all from Sigma-Aldrich (Merck KGaA, St. Louis, Missouri, USA).
Rtx 1ms
Manufactured by Restek
Sourced in United States, Australia
The Rtx-1MS is a high-performance gas chromatography (GC) column designed for a wide range of applications. It features a 100% dimethylpolysiloxane stationary phase, which provides excellent inertness and peak shape for a variety of analytes. The Rtx-1MS column is suitable for the analysis of a broad spectrum of organic compounds, including hydrocarbons, alcohols, esters, and more. It is available in a variety of lengths, internal diameters, and film thicknesses to accommodate different analytical requirements.
Automatically generated - may contain errors
Lab products found in correlation
Benzaldehyde, by Merck Group (1 mentions)
Phenethyl alcohol, by Merck Group (1 mentions)
Gcqp 2010 ultra, by Shimadzu (1 mentions)
Quattro micro, by Waters Corporation (1 mentions)
Db 5ms column, by Agilent Technologies (1 mentions)
Trace gc ultra, by Thermo Fisher Scientific (1 mentions)
Trace gc ultra apparatus, by Thermo Fisher Scientific (1 mentions)
Trace gc ultra, by Restek (1 mentions)
6 protocols using rtx 1ms
1
GC-MS Volatile Organic Compound Profiling
2
Multiresidue Analysis of Persistent Organic Pollutants
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An equivalent of 6 g liver of each extract was used for chemical analysis. 13 C-labeled standards of PCBs, PBDEs, new BFRs and pesticides (F1), surrogate standard MeSO2-4Me-PCB120 (F2) and 13 C-OH-PCBs/PCP (F3) were added before chemical analysis. Further, extract F3 was derivatized using diazomethane before final cleanup on a solid phase extraction (SPE)-column with 25% sulfuric acid silica, concentrated and transferred to an analytical vial GC/MS vial with a 200 µL insert.
PCBs, DDTs and PBDEs were analyzed by GC-MS (Waters Quattro micro) in EI mode in single ion monitoring using a 30m x 0.25mmID DB5-MS column (Agilent) for PCBs and DDTs and a 15m x 0.18mmID RTx1-MS (Restek) for PBDEs. The pesticides, MeSO2-PCBs/DDE and derivatized OH-PCBs/PCP were analyzed by GC/MS (Agilent 7890A/5975B MSD) in NCI mode in single ion monitoring, with methane as CI gas using a 30m x 0.25mmID DB5-MS column. OPFRs were analysed by LC-Q-TOF-MS after having spiked samples with internal standards, extracted in acetonitrile (1 ml) by repeated sonication and vortexing and cleaned up the supernatant using ENVI-Carb graphitized carbon absorbent and glacial acetic acid. For further information on analysis see Nøst et al. (2012) and Sandanger et al. (2004) .
PCBs, DDTs and PBDEs were analyzed by GC-MS (Waters Quattro micro) in EI mode in single ion monitoring using a 30m x 0.25mmID DB5-MS column (Agilent) for PCBs and DDTs and a 15m x 0.18mmID RTx1-MS (Restek) for PBDEs. The pesticides, MeSO2-PCBs/DDE and derivatized OH-PCBs/PCP were analyzed by GC/MS (Agilent 7890A/5975B MSD) in NCI mode in single ion monitoring, with methane as CI gas using a 30m x 0.25mmID DB5-MS column. OPFRs were analysed by LC-Q-TOF-MS after having spiked samples with internal standards, extracted in acetonitrile (1 ml) by repeated sonication and vortexing and cleaned up the supernatant using ENVI-Carb graphitized carbon absorbent and glacial acetic acid. For further information on analysis see Nøst et al. (2012) and Sandanger et al. (2004) .
3
Peppermint and Clove Oil Chemical Profiling
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Essential oils from Mentha piperita L. (peppermint oil) and Syzygium aromaticum (L.) Merr. & L.M. Perry (clove oil) were obtained from Pollena Aroma S.A., Warsaw, Poland.
The chemical compositions of the essential oils were analyzed by gas chromatography mass spectrometry flame ionization detection (GC-MS-FID) using a Trace GC Ultra (Thermo Scientific, Waltham, USA) chromatograph combined with a DSQ II mass spectrometer with a flame ionization detector (FID) using an MS-FID splitter (SGE, Analytical Science, Trajan, Australia) and a nonpolar capillary column, Rtx-1 ms (60 m × 0.25 mm, film thickness 0.25 μm, Restek, Bellefonte, PA, USA). The oven temperature was programmed as followed: 50–300 °C at 4 °C/min; injector temp.: 280 °C; detector temp.: 310 °C; carrier gas helium with regular pressure: 200 kPa; ionization energy: 70 eV; ion source temperature: 200 °C. Components were identified based on comparisons of their mass spectra with those of a laboratory-made MS library and commercial libraries (Adams [33 ], NIST 09, Wiley 275.1 and Mass Finder 4) and with retention indices associated with a series of alkanes using linear interpolation (C8–C26). Quantitative analyses (expressed as percentages of each component) were carried out using peak area-normalized measurements without correction factors. The identified essential oil components are presented inTable 3 .
The chemical compositions of the essential oils were analyzed by gas chromatography mass spectrometry flame ionization detection (GC-MS-FID) using a Trace GC Ultra (Thermo Scientific, Waltham, USA) chromatograph combined with a DSQ II mass spectrometer with a flame ionization detector (FID) using an MS-FID splitter (SGE, Analytical Science, Trajan, Australia) and a nonpolar capillary column, Rtx-1 ms (60 m × 0.25 mm, film thickness 0.25 μm, Restek, Bellefonte, PA, USA). The oven temperature was programmed as followed: 50–300 °C at 4 °C/min; injector temp.: 280 °C; detector temp.: 310 °C; carrier gas helium with regular pressure: 200 kPa; ionization energy: 70 eV; ion source temperature: 200 °C. Components were identified based on comparisons of their mass spectra with those of a laboratory-made MS library and commercial libraries (Adams [33 ], NIST 09, Wiley 275.1 and Mass Finder 4) and with retention indices associated with a series of alkanes using linear interpolation (C8–C26). Quantitative analyses (expressed as percentages of each component) were carried out using peak area-normalized measurements without correction factors. The identified essential oil components are presented in
4
GC-FID-MS Analysis of Salvia sclarea Oil
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Salvia sclarea L. (Lamiaceae) oil was obtained from POLLENA-AROMA Poland. It was analyzed by GC-FID-MS in the Institute of General Food Chemistry, Lodz University of Technology, using a Trace GC Ultra apparatus (Thermo Electron Corporation) with FID and MS DSQ II detectors and FID-MS splitter (SGE). Operating conditions: apolar capillary column Rtx-1ms (Restek), 60 m × 0.25 mm i.d., film thickness 0.25 µm; temperature program, 50–300°C at 4°C/min; SSL injector temperature 280°C; FID temperature 300°C; split ratio 1: 20; carrier gas helium at a regular pressure 200 kPa; FID temperature 260°C; carrier gas, helium; 0.5 ml/min; split ratio 1: 20. Mass spectra were acquired over the mass range 30–400 Da, ionization voltage 70 eV; ion source temperature 200°C.
Identification of components was based on the comparison of their MS spectra with those in a laboratory-made MS library, commercial libraries (NIST 98.1, Wiley Registry of Mass Spectral Data, 8th Ed. and MassFinder 4) and with literature data [22 , 23 ] along with the retention indices on an apolar column (Rtx-1, MassFinder 4) associated with a series of alkanes with linear interpolation (C8–C26).
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Salvia sclarea L. (Lamiaceae) oil was obtained from POLLENA-AROMA Poland. It was analyzed by GC-FID-MS in the Institute of General Food Chemistry, Lodz University of Technology, using a Trace GC Ultra apparatus (Thermo Electron Corporation) with FID and MS DSQ II detectors and FID-MS splitter (SGE). Operating conditions: apolar capillary column Rtx-1ms (Restek), 60 m × 0.25 mm i.d., film thickness 0.25 µm; temperature program, 50–300°C at 4°C/min; SSL injector temperature 280°C; FID temperature 300°C; split ratio 1: 20; carrier gas helium at a regular pressure 200 kPa; FID temperature 260°C; carrier gas, helium; 0.5 ml/min; split ratio 1: 20. Mass spectra were acquired over the mass range 30–400 Da, ionization voltage 70 eV; ion source temperature 200°C.
Identification of components was based on the comparison of their MS spectra with those in a laboratory-made MS library, commercial libraries (NIST 98.1, Wiley Registry of Mass Spectral Data, 8th Ed. and MassFinder 4) and with literature data [22 , 23 ] along with the retention indices on an apolar column (Rtx-1, MassFinder 4) associated with a series of alkanes with linear interpolation (C8–C26).
5
GC-MS Analysis of Essential Oils
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The essential oils of NR and HR roots (about 50 g of each) were obtained by hydrodistillation, using a clevenger-type apparatus for 5 h. Chemical analysis of essential oils composition was performed by GC-MS method according to Makowczyńska et al. [18 (link)]. Apparatus details are as follows: Trace GC Ultra with FID and MS DSQ II detector and MS-FID splitter, with column Rtx-1 ms (Restek), 60 m × 0.25 mm i.d., and film thickness 0.25 μm; temperature program, 50–310°C at 2°C/min; injector temp. 280°C; FID temp. 300°C; carrier gas helium and ionization voltage 70 eV; ion source temp. 200°C. The identification of the compounds was based on the comparison of their RIs and MS spectra with those stored in the computer libraries and literature data [19 , 20 ]. The percentages were computed from FID response without the use of correction factors. The results are showed in Table 1 .
6
Comprehensive GC-MS Analysis Protocol
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The primary column was a 30 m × 0.25 mm i.d. ×0.25 μm df Rtx‐1MS (Restek Corp.) and the secondary column a 2 m × 0.18 mm i.d. × 0.18 μm df Rxi‐17M (Restek Corp.). Carrier gas was helium, and runs were performed in splitless mode. The initial temperature of the GC oven was 40°C, held for 1 min, followed by ramping to 300°C at a rate of 5°C/min, followed by a 5‐min hold. Secondary oven temperature offset was +5°C to the primary oven, and modulator temperature offset was set to +20°C of the secondary oven. The thermal modulation periods were set to 5 s, hot pulse time 0.6 s, and cool time between stages 1.9 s. Column flow was set to 1.0 ml/min, and the total run time was set to 34.80 min. The transfer line was set to 300°C and ion source 225°C. Acquisition rate was set to 100 spectra/s with a 500‐s delay and mass range of 40–500 amu.
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