Yeast extract malt extract agar

Screening of actinobacteria from marine soil sediment sample was done by spread plate method (Manivasagan et al., 2013a , Manivasagan et al., 2013b ). The soil suspension was subjected to standard serial decimal dilution to give dilutions of 10⁻¹ to 10⁻¹⁰ using sterile 0.9% saline as diluents (Vimal et al., 2009 ). The actinobacteria from treated marine sediment samples was isolated by standard spread plating on the surface Starch Casein Agar (SCA; Hi-Media, India) (Selvakumar et al., 2012 ) supplemented with cycloheximide (25 mg/mL; SCAC) (Sivakumar et al., 2007 ) in level 1 bio safety cabinet. The whole procedure for each sample was done in triplicates to maximize the chance of isolating actinobacteria. The plates were dried for 25 min and incubated at 30 °C (4 weeks). Colonies showing typical actinobacteria growth characteristics were preserved by sub-culture on Yeast Extract Malt Extract Agar (Hi-Media) and stored under refrigerated conditions until further use.

The cultural characteristic of pure isolate was studied after incubation of strain ACTK2 for 7-14 days at 28˚C using various media: Tryptone Yeast Extract Agar (ISP-1), Yeast Extract-Malt Extract Agar (ISP-2), Oat Meal Agar (ISP-3), Starch-Casein nitrate Agar medium (ISP-4), Glycerol-Asparagine Agar (ISP-5), Peptone Yeast Extract Iron Agar (ISP-6), Modified ISP-2 (MISP-2), Starch Agar, Potato Dextrose Agar, Czapek Dox Agar, Nutrient Agar, Modified Nutrient Glucose Agar (MNGA) and Mueller Hinton Agar, procured from Hi-Media, Mumbai, India. Morphological parameters such as aerial and substrate mycelium, arrangements of spores and Gram staining reaction of the ACTK2 strain (16 ) were carried out as described in Bergy's Manual of Systematic Bacteriology and by Shirling and Gottlieb (17 , 18 (link)).