The percentage of living cells was determined using the Lactate Dehydragenase (LDH) Cytotoxicity Assay Kit (Beyotime Institute of Biotechnology, Jiangsu, China). In brief, HepG2 hepatocytes and C2C12 myotubes were cultured in 96-well plates. After berberine or metformin treatment for 24 h in DMEM supplemented with 0.25% BSA, LDH was measured in medium and cell extracts according to the manufacturer’s instruction.
Lactate dehydragenase ldh cytotoxicity assay kit
Manufactured by Beyotime
Sourced in China
The Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit is a colorimetric assay that measures the amount of lactate dehydrogenase released from damaged cells. It provides a simple and efficient method for quantifying cytotoxicity or cell death in cell-based assays.
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Lab products found in correlation
2 protocols using lactate dehydragenase ldh cytotoxicity assay kit
1
Cytotoxicity Assay for Berberine and Metformin
2
Evaluation of Cell Death and Cytokine Levels
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Cell death was evaluated using the Lactate Dehydragenase (LDH) Cytotoxicity Assay Kit (Beyotime Institute of Biotechnology, Jiangsu, China). SH-SY5Y cells were grown in 96-well plates. After OGD treatment for 6h, the level of LDH in the cell culture supernatant was measured according to the manufacturer’s instruction.
ELISA was used to assess the effects of MLIF on TNF-α and IL-1β levels in OGD-induced BV-2 cells. Cells were plated onto 12-well plate. Supernatants were harvested to measure IL-1β and TNF-α using ELISA kits according to the manufacturer’s instruction.
ELISA was used to assess the effects of MLIF on TNF-α and IL-1β levels in OGD-induced BV-2 cells. Cells were plated onto 12-well plate. Supernatants were harvested to measure IL-1β and TNF-α using ELISA kits according to the manufacturer’s instruction.
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