All laboratory measurements were performed after at least 12 h of fasting. Glycaemia was determined by the glucose oxidase method (glucose analyser, BeckmanCoulter, Milan). Blood levels of total cholesterol, low‐density lipoprotein (LDL) cholesterol, high‐density lipoprotein (HDL) cholesterol and triglycerides were analysed by enzymatic methods (Roche Diagnostics GmbH, Mannheim, Germany). Creatinine levels were measured using the Jaffe method. The estimation of glomerular filtration rate (eGFR) was based on the new CKD‐EPI (Chronic Kidney Disease Epidemiology Collaboration) equation.21 Serum uric acid (UA) levels were assessed using URICASE/POD method (Boehringer Mannheim, Mannheim, Germany). The high‐sensitivity C‐reactive protein (hs‐CRP) was quantified by the immunoturbidimetric method automated system (Cardio Phase hs‐CRP, Milan, Italy).
Uricase pod method
Manufactured by Roche
Sourced in Germany
The URICASE/POD method is a laboratory technique used for the quantitative determination of uric acid levels in biological samples. It involves the enzymatic conversion of uric acid to allantoin, which is then detected and measured colorimetrically. This method provides a reliable and accurate way to assess uric acid concentrations in various clinical and research applications.
Automatically generated - may contain errors
9 protocols using uricase pod method
1
Comprehensive Metabolic Profiling Protocol
2
Fasting Metabolic Biomarkers Assessment
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At the first eligibility visit, all laboratory measurements were performed after a fasting period of at least 12 h. Plasma glucose was determined by the glucose oxidase method (Glucose Analyzer, Beckman Coulter SpA, Milan, Italy). Triglyceride and cholesterol concentrations were measured by enzymatic methods (Roche Diagnostics GmbH, Mannheim, Germany). Serum creatinine was measured by an automated technique based on the measurement of Jaffe chromogen and by the URICASE/POD method (Boehringer Mannheim, Mannheim, Germany) implemented in an auto-analyzer. Values of e-GFR were calculated by using the equation proposed by CKD-EPI. hs-CRP was measured by a turbidimetric immunoassay (Behring).
Insulin resistance (IR) was estimated by homeostasis model assessment (HOMA-IR) according to the following equation:
HOMA = [insulin (μU/mL) × glucose (mmol/L)]/22.5 [15 (link)].
Insulin resistance (IR) was estimated by homeostasis model assessment (HOMA-IR) according to the following equation:
HOMA = [insulin (μU/mL) × glucose (mmol/L)]/22.5 [15 (link)].
3
Serum Creatinine and Uric Acid Assessment
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Serum creatinine and uric acid (UA) were measured by an automated technique based on the measurement of Jaffe chromogen and by the URICASE/POD method (Boehringer Mannheim, Mannheim Germany) implemented in an auto-analyzer. Values of e-GFR were calculated by the CKD-Epidemiology Collaboration proposed equation that is more accurate in subjects with e-GFR > 60 ml/min/1.73 m2 (link),37 (link).
4
Fasting Laboratory Measurements Protocol
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At the first eligibility visit, all laboratory measurements were performed after a fasting period of at least 12 h. Total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride, and fasting glucose were measured using the standard methods (Roche Diagnostics GmbH, Mannheim, Germany, and Glucose Analyzer, Beckman Coulter SpA, Milan, Italy). Serum creatinine was measured by an automated technique based on the measurement of Jaffe chromogen and the URICASE/POD method (Boehringer Mannheim, Mannheim, Germany) implemented in an auto-analyzer. Values of e-GFR were calculated using the equation proposed by investigators in Chronic Kidney Disease Epidemiology (CKD-EPI). High-sensitivity C-reactive protein (hs-CRP) was measured by a turbidimetric immunoassay (Behring, Montgomery, PA, USA).
5
Comprehensive Metabolic Profile Analysis
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All laboratory measurements were performed on peripheral blood samples after at least 12 h of fasting. SA was measured with a colorimetric spectrophotometric method (Bromocresol green). Glycaemia was determined by the glucose oxidase method (glucose analyzer, BeckmanCoulter, Milan) and the homeostasis model assessment (HOMA) index was used for the determination of Insulin resistance [34] (link). Enzymatic methods (Roche Diagnostics GmbH, Mannheim, Germany) were used for determination of blood levels of total cholesterol, low-density lipoprotein (LDL) cholesterol, highdensity lipoprotein (HDL) cholesterol, and triglycerides. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) by pyridoxal phosphate activated (liquid reagent), and gamma-glutamyltransferase (γ-GT) were evaluated by standardized method (COBAS Integra 800-Roche Diagnostics GmbH, Mannheim, Germany). Creatinine was measured using the Jaffé method. The CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation was used for the estimation of glomerular filtration rate (eGFR) [35] (link). Serum uric acid (UA) levels were assessed using URICASE/POD method (Boehringer Mannheim, Mannheim, Germany). The immuno-turbidimetric method automated system (Cardio Phase hs-CRP, Milan, Italy) was used to assess the highsensitivity C-reactive protein (hs-CRP).
6
Fasting Biochemical Measurements in Patients
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At the first eligibility visit, all laboratory measurements were performed after a fasting period of at least 12 h. Plasma glucose was determined by the glucose oxidase method (glucose analyser, Beckman Coulter SpA, Milan, Italy). Triglyceride and cholesterol concentrations were measured by enzymatic methods (Roche Diagnostics GmbH, Mannheim, Germany). Serum creatinine was measured by an automated technique based on the measurement of Jaffe chromogen and by the URICASE/POD method (Boehringer Mannheim, Mannheim, Germany) implemented in an auto‐analyser. Values of e‐GFR were calculated by using the equation proposed by investigators in the Chronic Kidney Disease Epidemiology. High‐sensitivity C‐reactive protein (hs‐CRP) was measured, in 653 patients, by a turbidimetric immunoassay (Behring).
7
Fasting Metabolic Biomarkers Measurement
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All laboratory measurements were performed after a fasting period of at least 12 h. Plasma glucose was determined by the glucose oxidase method (Glucose Analyzer, Beckman Coulter SpA, Milan, Italy). Triglyceride and total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol concentrations were measured by enzymatic methods (Roche Diagnostics GmbH, Mannheim, Germany). Serum creatinine was measured by an automated technique based on the measurement of Jaffe chromogen and by the URICASE/POD method (Boehringer Mannheim, Mannheim, Germany) implemented in an auto-analyzer. Values of e-GFR were calculated by using the equation proposed by investigators in the Chronic Kidney Disease Epidemiology (CKD-EPI) Collaboration38 (link). High-sensitivity C-reactive protein (hs-CRP) was measured by a turbidimetric immunoassay (Behring).
8
Fasting Metabolic Biomarker Assessment
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All laboratory measurements were performed after at least 12 h of fasting. Glycaemia was determined by the glucose oxidase method (glucose analyser, BeckmanCoulter, Milan). Blood levels of total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol and triglycerides were analysed by enzymatic methods (Roche Diagnostics GmbH, Mannheim, Germany). Creatinine levels were measured using the Jaffe method. The estimation of glomerular filtration rate (eGFR) was based on the new CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation. 21 Serum uric acid (UA) levels were assessed using URICASE/POD method (Boehringer Mannheim, Mannheim, Germany). The high-sensitivity C-reactive protein (hs-CRP) was quantified by the immunoturbidimetric method automated system (Cardio Phase hs-CRP, Milan, Italy).
9
Metabolic Profile Biomarker Analysis
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All laboratory measurements were carried out on peripheral blood samples after at least 12 h of fasting. Glycemia was determined by the glucose oxidase method (glucose analyzer, BeckmanCoulter, Milan). Creatinine levels were measured using the Jaffe method. The estimation of glomerular filtration rate (eGFR) was based on the new CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation [42] (link). Serum uric acid (UA) levels were assessed using URICASE/POD method (Boehringer Mannheim, Mannheim, Germany). Serum levels of high-sensitivity C-reactive protein (hs-CRP) were measured by immunoturbidimetric method automated system (Cardio Phase hs-CRP, Milan, Italy). In addition, glycated hemoglobin (HbA1c) was measured by high-performance liquid chromatography certified by the national glycohemoglobin standardization program (NGSP) and using an automatic analyzer (Adams HA-8160 HbA1c analyzer, Menarini, Italy). Analytical determinations were taken using an automatic particle counter (Siemens Healthcare Diagnostics, ADVIA 120/2120 Haematology System, Milan, Italy) to measure haemoglobin, haematocrit, and white blood cell count. Plasma concentrations of insulin were determined by chemiluminescence test (Roche Diagnostics). Insulin resistance was determined by the homeostasis model assessment (HOMA) index [43] (link).
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