Quantikine high sensitivity elisa human tnf α immunoassay

Serum levels of DHEA-S were measured using a chemiluminescent enzyme immunoassay (CLEIA). Cortisol and prolactin (PRL) were measured by electro-chemiluminescence immunoassay. Total carnitine, acylcarnitine, and free carnitine were measured by the enzyme cycling method. IL-6 was measured using a human IL-6 CLEIA cartridge (Fujirebio, Tokyo, Japan), with a minimum detectable concentration of 0.2 pg/mL. TNF-α was measured by enzyme-linked immunosorbent assay (ELISA) using Quantikine high-sensitivity ELISA human TNF-α immunoassay (R&D Systems, Inc., Minneapolis, MN, USA), with a minimum detectable concentration of 0.07 pg/mL. IFN-α was measured using the VeriKine™ Human Interferon Alpha Multi-Subtype Serum ELISA Kit (pbl assay science, Piscataway, NJ, USA), with a minimum detectable concentration of 12.5 pg/mL. IFN-γ was measured by enzyme amplified sensitivity immunoassay (EASIA) using the MEDGENIX human IFN-γ EASIA kit (BioSource Europe S.A., Nivelles, Belgium), with a minimum detectable concentration of 0.1 IU/mL.

Serum DHEA-S levels were measured by chemiluminescent enzyme immunoassay and cortisol and PRL levels by an electrochemiluminescence immunoassay. TNF-α levels were measured by an enzyme-linked immunosorbent assay (ELISA) using the Quantikine high-sensitivity ELISA human TNF-α immunoassay (R&D Systems, Inc., Minneapolis, MN, USA), with a minimum detectable concentration of 0.07 pg/mL. IL-6 levels were measured using a human IL-6 chemiluminescent enzyme immunoassay cartridge (Fujirebio, Tokyo, Japan), with a minimum detectable concentration of 0.2 pg/mL. Total carnitine, acylcarnitine and free carnitine concentrations were measured using the enzyme cycling method.