Msm mc2155 was grown in liquid media containing 7H9 salts (Becton Dickinson) supplemented with 5 g/L albumin, 2 g/L dextrose, 0.85 g/L NaCl, 0.003 g/L catalase, 0.2% glycerol, and 0.05% Tween80, or plated on LB agar. For all oxidative stress experiments, strains were grown in Hartmans-de Bont (HdB) media, which was made as described (Hartmans and De Bont, 1992 ) with 0.05% Tween80. E. coli TOP10 was used for cloning. Antibiotic selection concentrations for M. smegmatis were as follows: 25 μg/mL kanamycin, 50 μg/mL hygromycin, 20 μg/mL zeocin, 20 μg/mL nourseothricin, and 5 μg/mL gentamicin. Antibiotic concentrations for E. coli were as follows: 50 μg/mL kanamycin, 100 μg/mL hygromycin, 50 μg/mL zeocin, and 40 μg/mL nourseothricin. Anhydrotetracyline was used at 100 ng/mL for gene induction or repression. All strains were grown at 37°C unless otherwise indicated.
7h9 salts
Manufactured by BD
7H9 salts are a type of laboratory media used for the cultivation of various bacteria, particularly Mycobacterium species. The salts provide the necessary nutrients and growth factors for the bacteria to thrive in a controlled laboratory environment. The composition and formulation of the 7H9 salts are designed to support the optimal growth and maintenance of the target microorganisms.
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Lab products found in correlation
3 protocols using 7h9 salts
1
Growth Conditions and Antibiotic Selection for Mycobacterium Smegmatis
2
Growth Conditions and Antibiotic Selection for Mycobacterium Smegmatis
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Msm mc2155 was grown in liquid media containing 7H9 salts (Becton Dickinson) supplemented with 5 g/L albumin, 2 g/L dextrose, 0.85 g/L NaCl, 0.003 g/L catalase, 0.2% glycerol, and 0.05% Tween80, or plated on LB agar. For all oxidative stress experiments, strains were grown in Hartmans-de Bont (HdB) media, which was made as described (Hartmans and De Bont, 1992 ) with 0.05% Tween80. E. coli TOP10 was used for cloning. Antibiotic selection concentrations for M. smegmatis were as follows: 25 μg/mL kanamycin, 50 μg/mL hygromycin, 20 μg/mL zeocin, 20 μg/mL nourseothricin, and 5 μg/mL gentamicin. Antibiotic concentrations for E. coli were as follows: 50 μg/mL kanamycin, 100 μg/mL hygromycin, 50 μg/mL zeocin, and 40 μg/mL nourseothricin. Anhydrotetracyline was used at 100 ng/mL for gene induction or repression. All strains were grown at 37°C unless otherwise indicated.
3
Growth Conditions for Mycobacterium and E. coli
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Mycobacterium smegmatis mc2155 was grown in 7H9 salts (Becton-Dickinson, Franklin Lakes, NJ) supplemented with: 5 g/L albumin, 2 g/L dextrose, 0.85 g/L NaCl, 0.003 g/L catalase, 0.2% glycerol and 0.05% Tween80, or plated on LB agar. Hartmans-de Bont (HdB) media was made as described (Hartmans and De Bont, 1992 ) with 0.05% tween80, HdB-C was made without glycerol. E. coli DH5α was used for cloning and E. coli BL21 codon plus or ArcticExpress DE3 RP were used for protein expression. Antibiotic concentrations for M. smegmatis were: 25 µg/ml kanamycin, 50 µg/ml hygromycin, 20 µg/ml zeocin and 20 µg/ml nourseothricin. Antibiotic concentrations of E. coli were: 50 µg/ml kanamycin, 100 µg/ml hygromycin, 25 µg/ml zeocin, 40 µg/ml nourseothricin, 20 µg/ml chloramphenicol and 140 µg/ml ampicillin. All strains were grown at 37°C.
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