Components for the magnet rack were designed using Autodesk Inventor software and printed with an Objet30 3D printer using VeroGray resin. Strong 1-inch neodymium magnets (K&J magnetics) were inserted into the rack, which was then sealed. CS-MACS (Miltenyi Biotec) columns are filled with RPMI from a 2-way stopcock then inserted into the magnet-holder. Columns were washed with 10mL RPMI before use, then attached to 23 gauge blunt-ended syringe filters to control flow speed. The RBC suspension was added to the column at a hematocrit of under 20%, then washed with RPMI. Once the flowthrough ran clear, the columns were washed with an additional 20mL of RPMI. The columns were removed from the magnet and placed in 15mL tubes, and late-stage iRBCs were eluted with 10mL of RMPI, with the syringe tips still attached. Purity of both the elution and the flowthrough were checked by giemsa smear. If parasitemia in the elution was under 90%, the columns were returned to the magnet-holder and the eluate passed through one more time, with 10mL RPMI for washing, before a final elution with 10mL RPMI. Columns were washed with 100mL H2O followed by 10mL 70% EtOH, then dried and stored in a 37°C incubator.
Cs macs
Manufactured by Miltenyi Biotec
The CS-MACS is a magnetic cell separation device designed for the isolation and enrichment of specific cell populations from complex biological samples. It utilizes magnetic microbeads coated with specific antibodies or ligands to label the target cells, which are then separated using a magnetic field.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cs macs
1
Magnetic Separation of Late-Stage Malaria-Infected RBCs
2
Magnetic Separation of Late-Stage Malaria-Infected RBCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Components for the magnet rack were designed using Autodesk Inventor software and printed with an Objet30 3D printer using VeroGray resin. Strong 1-inch neodymium magnets (K&J magnetics) were inserted into the rack, which was then sealed. CS-MACS (Miltenyi Biotec) columns are filled with RPMI from a 2-way stopcock then inserted into the magnet-holder. Columns were washed with 10mL RPMI before use, then attached to 23 gauge blunt-ended syringe filters to control flow speed. The RBC suspension was added to the column at a hematocrit of under 20%, then washed with RPMI. Once the flowthrough ran clear, the columns were washed with an additional 20mL of RPMI. The columns were removed from the magnet and placed in 15mL tubes, and late-stage iRBCs were eluted with 10mL of RMPI, with the syringe tips still attached. Purity of both the elution and the flowthrough were checked by giemsa smear. If parasitemia in the elution was under 90%, the columns were returned to the magnet-holder and the eluate passed through one more time, with 10mL RPMI for washing, before a final elution with 10mL RPMI. Columns were washed with 100mL H2O followed by 10mL 70% EtOH, then dried and stored in a 37°C incubator.
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