Log In Sign up
The largest database of trusted experimental protocols

Fibronectin sirna

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

Fibronectin siRNA is a gene silencing tool that targets the expression of the fibronectin gene. It is designed to reduce the production of the fibronectin protein in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Opti mem, by Thermo Fisher Scientific (3 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Integrin β1, by Cell Signaling Technology (2 mentions) Integrin α5, by Cell Signaling Technology (2 mentions) Integrin α5 sirna, by Santa Cruz Biotechnology (2 mentions) Fibronectin pool, by Santa Cruz Biotechnology (2 mentions) Integrin β1 sirna, by Santa Cruz Biotechnology (2 mentions) Integrin β1 sirna b, by Santa Cruz Biotechnology (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Fibronectin (189 citations) SiRNAs (95 citations)

3 protocols using fibronectin sirna

1

Integrin and Fibronectin Knockdown Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated and left to grow overnight in 5% FBS/RPMI. 5%FBS/RPMI media was replaced with Opti-MEM (Invitrogen). Fibronectin pool, Fibronectin siRNA A GACUGGUGGUUACAUGUUAtt, Fibronectin siRNA B CGCAUCACUUGCACUUCUAtt, Fibronectin siRNA C GAUCCUGUCUACUUCACAAtt, Integrin α5 siRNA A GUCAGAAUUUCGAGACAAAtt, Integrin α5 siRNA B CCACUGACCAGAACUAGAAtt, Integrin β1 siRNA A GAGAUGAGGUUCAAUUUGAtt, Integrin β1 siRNA B GAUGAGGUUCAAUUUGAAAtt, (25nM, Santa Cruz), Integrin α5, Integrin β1, and PTEN (all 25nM, Cell Signaling Technologies), and BRAF (25nM, Thermo Scientific) siRNAs in complex with Lipofectamine 2000 (Invitrogen) were added. Scrambled, non-targeting siRNAs were used as controls. A final concentration of 5% FBS in RPMI was added the next day. For fibronectin knockdowns, final concentration 1% FBS in RPMI was added next day. Cells were transfected for 24-72 hours prior to treatment.
Fedorenko I.V., Abel E.V., Koomen J.M., Fang B., Wood E.R., Chen Y.A., Fisher K.J., Iyengar S., Dahlman K.B., Wargo J.A., Flaherty K.T., Sosman J.A., Sondak V.K., Messina J.L., Gibney G.T, & Smalley K.S. (2015). Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene, 35(10), 1225-1235.
+ Open protocol
+ Expand
2

Integrin and Fibronectin Knockdown Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated and left to grow overnight in 5% FBS/RPMI. 5%FBS/RPMI media was replaced with Opti-MEM (Invitrogen). Fibronectin pool, Fibronectin siRNA A GACUGGUGGUUACAUGUUAtt, Fibronectin siRNA B CGCAUCACUUGCACUUCUAtt, Fibronectin siRNA C GAUCCUGUCUACUUCACAAtt, Integrin α5 siRNA A GUCAGAAUUUCGAGACAAAtt, Integrin α5 siRNA B CCACUGACCAGAACUAGAAtt, Integrin β1 siRNA A GAGAUGAGGUUCAAUUUGAtt, Integrin β1 siRNA B GAUGAGGUUCAAUUUGAAAtt, (25nM, Santa Cruz), Integrin α5, Integrin β1, and PTEN (all 25nM, Cell Signaling Technologies), and BRAF (25nM, Thermo Scientific) siRNAs in complex with Lipofectamine 2000 (Invitrogen) were added. Scrambled, non-targeting siRNAs were used as controls. A final concentration of 5% FBS in RPMI was added the next day. For fibronectin knockdowns, final concentration 1% FBS in RPMI was added next day. Cells were transfected for 24-72 hours prior to treatment.
Fedorenko I.V., Abel E.V., Koomen J.M., Fang B., Wood E.R., Chen Y.A., Fisher K.J., Iyengar S., Dahlman K.B., Wargo J.A., Flaherty K.T., Sosman J.A., Sondak V.K., Messina J.L., Gibney G.T, & Smalley K.S. (2015). Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene, 35(10), 1225-1235.
+ Open protocol
+ Expand
3

Fibronectin Silencing in Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown in 6-well plates. When a confluency of 60–70% was reached, the cells were transfected according to the manufacturer’s instructions. Briefly, the cells were transfected with 10 nM of either fibronectin siRNA (Santa Cruz Biotechnology, TX, USA, cat#: sc-29315) or a non-targeting scramble control (Sigma, St.Louis, MO, USA) using Lipofectamine RNAiMAX (Thermo Fisher Scientific Inc.; Wilmington, DE, USA) diluted in Opti-MEM (ThermoFisher Scientific Inc.; Wilmington, DE, USA). The cells were then incubated for 24 h at 37 °C after which cells were harvested.
Huaman J., Naidoo M., Zang X, & Ogunwobi O.O. (2019). Fibronectin Regulation of Integrin B1 and SLUG in Circulating Tumor Cells. Cells, 8(6), 618.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!