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Goat anti e cadherin

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Goat anti-E-cadherin is a primary antibody that recognizes the E-cadherin protein. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in maintaining cell-cell junctions and epithelial cell polarity. This antibody can be used to detect and study the expression and localization of E-cadherin in various experimental systems.

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Lab products found in correlation

Mouse anti cdx2, by BioGenex (2 mentions) Rabbit anti p53, by Vector Laboratories (2 mentions) Rabbit anti vimentin, by Abcam (2 mentions) Mouse anti β catenin, by BD (2 mentions) Irdye conjugated antibody, by LI COR (1 mentions) Goat anti lamin b1, by Santa Cruz Biotechnology (1 mentions) Hoechst 33342 solution 20 mm, by Thermo Fisher Scientific (1 mentions) Mouse anti brdu, by Cell Signaling Technology (1 mentions) 7 aminoactinomycin d 7 aad, by BD (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Smart pool of 4 sirnas, by Horizon Discovery (1 mentions) Mouse igg2a, by R&D Systems (1 mentions) Mouse anti cd81, by BD (1 mentions) Rabbit anti ocln, by Abcam (1 mentions) Rabbit anti pkc substrate, by Cell Signaling Technology (1 mentions) Normal goat igg control, by R&D Systems (1 mentions) Mouse igg1 isotype control, by R&D Systems (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions) Murine recombinant epidermal growth factor, by Thermo Fisher Scientific (1 mentions) Growth factor reduced matrigel, by Avantor (1 mentions)

Spelling variants of this product

E-cadherin (59 citations) Anti-E-cadherin (26 citations) Goat anti-E-cadherin pAb (2 citations)

9 protocols using goat anti e cadherin

1

Multimodal Analysis of Cell Proliferation

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Primary antibodies were as follows: rabbit anti-MCM2 and anti-MCM7 (Cell Signaling Technology, catalog 3619 and 3735); goat anti–lamin B1 (Santa Cruz Biotechnology, catalog sc-6217); mouse anti-BrdU (Cell Signaling Technology, catalog 5292); mouse anti-GAPDH and anti-HSP90 (OriGene, catalog TA802519 and TA500494); mouse APC-conjugated anti-BrdU (BioLegend, catalog 364113); and goat anti–E-cadherin (R&D Systems, catalog AF648). 7-Amino-actinomycin D (7-AAD) was from BD Biosciences (catalog 559925). Hoechst 33342 Solution 20 mM was from Thermo Fisher Scientific (catalog 62249). Secondary IRDye-conjugated antibodies and Odyssey IR imager with Image Studio software were from LI-COR Biosciences.
Rochman M., Rochman Y., Caldwell J.M., Mack L.E., Besse J.A., Manes N.P., Yoon S.H., Shoda T., Nita-Lazar A, & Rothenberg M.E. (2023). The minichromosome maintenance complex drives esophageal basal zone hyperplasia. JCI Insight, 8(17), e172143.
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2

Antibody Purification and Immunoblotting Protocol

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Murine monoclonal anti–human JAM-A (J10.4) was purified as described previously (Liu et al., 2000 (link)). Rabbit anti-p-JAM-A Y280 was from Rockland, Limerick, PA, catalogue: 600-401-GN5. Other antibodies were purchased: goat anti–JAM-A, mouse and goat anti-PTPN13, goat anti–E-cadherin (R & D Systems, Minneapolis, MN); polyclonal rabbit anti–JAM-A, ZO-2 and monoclonal mouse anti–ZO-2 (Thermo Fisher Scientific, Waltham, MA); monoclonal mouse anti–β catenin, mouse anti–Rap-2, Yes-1 (BD Transduction Laboratories, Lexington, KY); polyclonal rabbit anti-Src, Yes-1, Lyn, p-Src Y416, p-EGFR Y1068, p-HER3 Y1289 (Cell Signaling Technology, Beverly, MA); polyclonal rabbit anti-HA, calnexin, and monoclonal mouse anti-HA (Sigma-Aldrich, St. Louis, MO); polyclonal rabbit anti–Fap-1 (Santa Cruz, Dallas, Texas). For immunoblots, horseradish peroxidase–conjugated secondary antibodies were purchased (Jackson Immuno-Research Laboratories, West Grove, PA). For immunofluorescence, FITC and Alexa-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA) were used.
Fan S., Weight C.M., Luissint A.C., Hilgarth R.S., Brazil J.C., Ettel M., Nusrat A, & Parkos C.A. (2019). Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation. Molecular Biology of the Cell, 30(5), 566-578.
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3

TACSTD2 Gene Overexpression and Silencing

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A pcDNA3.1 vector carrying the TACSTD2 gene insert and a pcDNA3.1 vector carrying the siRNA-resistant TACSTD2 gene devoid of the 3’- and 5’-untraslated regions (UTR) (pTACSTD2) for rescue experiments were purchased from GenScript Corporation. siRNA for TACSTD2 gene silencing (siTACSTD2) targeting the 3’UTR of the TACSTD2 gene was performed using SMARTpool of 4 siRNAs (Dharmacon) (siTD2-A, 5’-GAGAAGAGGAGUUUGUUAAUU-3’; siTD2-B, 5’-ACAAGUAUCUGUAUGACAAUU-3’, siTD2-C, 5’-GCAAGUAACUGAAUCCAUUUU-3’, siTD2-D, 5’-GCACACACCAGGUUUAAUAUU-3’), which were transfected into parental and TACSTD2-overexpressing Huh 7.5 cells at 100nM final concentration using RNAiMAX (Invitrogen). The antibodies used include mouse anti-TACSTD2 (Santa Cruz), goat anti-TACSTD2, biotinylated goat anti-TACSTD2 and goat anti-E-cadherin, mouse IgG1 isotype control, mouse IgG2A isotype control, and normal goat IgG control (R&D systems), mouse anti-CLDN1 (Abnova), rabbit anti-CLDN1 and mouse anti-OCLN (Life Technologies), rabbit anti-OCLN (Abcam), rabbit anti-CD81 and mouse anti-ZO-1 (Thermo Scientific), mouse anti-SR-B1 (BD transduction laboratories), mouse anti-CD81 (BD Pharmingen), mouse anti-JAM-A (Hycult Biotech), mouse anti-HCV core (Anogen), rabbit anti-PKC substrate (Cell Signaling Technologies), and rabbit anti-alpha tubulin (Millipore). The HCV neutralizing mAb AR4A was a gift from Dr. Mansun Law.
Sekhar V., Pollicino T., Diaz G., Engle R.E., Alayli F., Melis M., Kabat J., Tice A., Pomerenke A., Altan-Bonnet N., Zamboni F., Lusso P., Emerson S.U, & Farci P. (2018). Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma. PLoS Pathogens, 14(3), e1006916.
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4

Immunohistochemical Analysis of Mouse Tissue

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Mouse tissues were fixed in 10% (v/v) buffered formalin and embedded in paraffin. Sections of the paraffin-embedded tissues were subjected to immunohistochemical (IHC) analysis as described[8 (link)]. The following primary antibodies were used for IHC analysis: mouse anti–β-catenin (1:800; BD Biosciences, Lexington, KY), rabbit anti-p53 (1:500; Vector Laboratories Inc., Burlingame, CA), goat anti–E-cadherin (1:500; R&D Systems, Minneapolis, MN), Rabbit anti-vimentin (1:500; Epitomics, Burlingame, CA), and mouse anti-CDX2 (1:100; BioGenex, Fremont, CA). The IHC staining with anti-p53 antibody was classified according to the percentage of stained cells. Expression of p53 was considered to be weak if <10% of neoplastic cells were observed to be stained, and strong if >10% of neoplastic cells were stained.
Tang J., Feng Y., Kuick R., Green M., Green M., Sakamoto N., Kurosu Y., Lin J., Cho K.R, & Fearon E.R. (2019). Trp53 null and R270H mutant alleles have comparable effects in regulating invasion, metastasis and gene expression in mouse colon tumorigenesis. Laboratory investigation; a journal of technical methods and pathology, 99(10), 1454-1469.
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5

Intestinal Organoid Culture and Immunostaining

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Crypt culture media and supplements; Advanced DMEM/F12, Glutamax and B27 and N2 were purchased from InVitrogen. Murine recombinant epidermal growth factor, noggin and IL-6, IL-22 were all obtained from Peprotech and mouse recombinant R-spondin 1 from R & D Systems. Growth factor-reduced Matrigel was purchased from VWR International.
Immunolabelling; primary and secondary antibodies; rat anti-BrdU (Abcam), mouse anti-Lysozyme (Abcam); goat anti-E-cadherin (R&D); rabbit IgG, rabbit anti IL6 receptor and anti-IL-6 (Bio-Xcell), rabbit anti-pSTAT3 Tyr705, pSTAT3 Y705 blocking peptide, rabbit anti-Cleaved caspase-3 (Cell Signaling); Immunolabelling was visualised by using an appropriate combination of species-specific Alexafluor-conjugated secondary antibodies (488, 568, and 647 nm), raised in mouse, donkey or goat (Invitrogen). FITC-conjugated Ulex europaeus Lectin (UEA-1 FITC) was purchased from Sigma. Vectashield mounting medium with DAPI was purchased from Vector Laboratories Ltd. STATTIC and IWP2 were purchased from Tocris Bioscience.
Jeffery V., Goldson A.J., Dainty J.R., Chieppa M, & Sobolewski A. (2017). Interleukin-6 signaling regulates small intestinal crypt homeostasis. Journal of immunology (Baltimore, Md. : 1950), 199(1), 304-311.
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6

Immunostaining Protocol for Tongue and Ganglion Sections

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Immunoreactions were performed as described previously [26, (link)27] (link). Briefly, tongue or ganglion sections were air dried, rehydrated, blocked (in 10% normal donkey serum, 0.3% Triton-X in PBS-X), and incubated overnight at 4˚C with primary antibodies. On the next day, slides were washed and incubated with appropriate secondary antibodies for 1-2 h at room temperature in the dark. Primary antibodies were goat anti-SHH (AF464, 0.1 μg/mL; R&D Systems); rat anti-keratin 8 (TROMA-1, 1:1,000; Developmental Studies Hybridoma Bank); goat anti-Ecadherin (AF748, 1:5,000; R&D Systems) and rabbit anti-RFP (600-401-379, 1:1,000; Rockland). For SHH in tongue sections, the heat-induced antigen-retrieval method [26] (link) was used.
Kumari A., Franks N.E., Li L., Audu G., Liskowicz S., Johnson J.D., Mistretta C.M, & Allen B.L. (2024). Distinct expression patterns of Hedgehog signaling components in mouse gustatory system during postnatal tongue development and adult homeostasis. PloS one, 19(6).
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7

Antibody Generation and Characterization

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Rat anti–mouse GP2 antibody was generated in our laboratory (2F11-3). Rabbit anti–Muc2 (H-300), rabbit anti–TRAF6 (H-274), and goat anti–Annexin V (R-20) antibodies were purchased from Santa Cruz Biotechnology. Rabbit anti–RelB monoclonal antibody (D7D7W) was purchased from Cell Signaling Technology. Rabbit anti–Marcksl1 antibody was purchased from Proteintech. Goat anti–E-cadherin, goat anti–CCL20, and sheep anti-Spi-B antibodies were obtained from R&D Systems. Donkey Dylight549 anti–rat IgG antibody and Dylight488 anti–rabbit IgG were purchased from Jackson ImmunoResearch. Donkey Alexa Fluor 555 and 633 anti–goat IgG were purchased from Life Technologies.
Kanaya T., Sakakibara S., Jinnohara T., Hachisuka M., Tachibana N., Hidano S., Kobayashi T., Kimura S., Iwanaga T., Nakagawa T., Katsuno T., Kato N., Akiyama T., Sato T., Williams I.R, & Ohno H. (2018). Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling. The Journal of Experimental Medicine, 215(2), 501-519.
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8

Liver Protein Extraction and Western Blotting

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Total liver and isolated hepatocyte lysates were prepared using RIPA buffer with protease inhibitor cocktail (Sigma-Aldrich Corp. St Louis, MO). For western blotting, primary antibodies used included rabbit anti-pSmad2 (Abcam or Cell Signaling as above), goat anti-E-cadherin (R & D), rabbit anti-albumin (Santa Cruz), rabbit anti- α SMA (Abcam), and rabbit anti-Yap1 (Cell Signaling). HRP-conjugated anti-rabbit or mouse (GE Healthcare, Pittsburgh, PA) and donkey anti-goat (Santa Cruz) were used as secondary antibodies. Signal was developed using ECL Prime (GE Healthcare) with detection on a Chemidoc system (BioRad) or using DAB reagent (DAKO).
Oh S.H., Swiderska-Syn M., Jewell M.L., Premont R.T, & Diehl A.M. (2018). Liver regeneration requires Yap1-TGFβ-dependent epithelial-mesenchymal transition in hepatocytes. Journal of hepatology, 69(2), 359-367.
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9

Immunohistochemical Analysis of Mouse Tissue

Cited 1 time
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Mouse tissues were fixed in 10% (v/v) buffered formalin and embedded in paraffin. Sections of the paraffin-embedded tissues were subjected to immunohistochemical (IHC) analysis as described[8 (link)]. The following primary antibodies were used for IHC analysis: mouse anti–β-catenin (1:800; BD Biosciences, Lexington, KY), rabbit anti-p53 (1:500; Vector Laboratories Inc., Burlingame, CA), goat anti–E-cadherin (1:500; R&D Systems, Minneapolis, MN), Rabbit anti-vimentin (1:500; Epitomics, Burlingame, CA), and mouse anti-CDX2 (1:100; BioGenex, Fremont, CA). The IHC staining with anti-p53 antibody was classified according to the percentage of stained cells. Expression of p53 was considered to be weak if <10% of neoplastic cells were observed to be stained, and strong if >10% of neoplastic cells were stained.
Tang J., Feng Y., Kuick R., Green M., Green M., Sakamoto N., Kurosu Y., Lin J., Cho K.R, & Fearon E.R. (2019). Trp53 null and R270H mutant alleles have comparable effects in regulating invasion, metastasis and gene expression in mouse colon tumorigenesis. Laboratory investigation; a journal of technical methods and pathology, 99(10), 1454-1469.
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