The number of sperm cells in a sample is critical for the determination of the GC density. Sperm cells in a suspension were counted with a Neubauer cell counter and a CASY Cell Counter system (Roche). Cell counts obtained by either method were not statistically different. The sperm number ranged from 0.7 × 1010 to 8.7 × 1010 sperm cells/milliliter dry sperm; the mean was 3.4 ± 1.8 × 1010 cells/milliliter (19 animals; Fig. S5 ).
Casy cell counter system
Manufactured by Roche
The CASY Cell Counter system is a laboratory equipment designed for the automated counting and analysis of cells. It utilizes a specialized technology to provide accurate and reliable cell count measurements. The system is capable of measuring cell size, concentration, and viability, making it a versatile tool for various applications in cell biology and cell-based research.
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Lab products found in correlation
2 protocols using casy cell counter system
1
Sperm Cell Counting for GC Density
2
Cultivation and Isolation of Trypanosoma brucei rhodesiense
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Trypanosoma brucei rhodesiense STIB900 is a derivative of STIB704, isolated from a male patient at St. Francis Hospital in Ifakara, Tanzania, in 1981. After several passages in rodents and a cyclic passage through a tsetse fly (Glossina morsitans morsitans), a cloned population was adapted to axenic growth. T. b. rhodesiense STIB900-M and STIB900-P had been selected independently in vitro for resistance to melarsoprol and pentamidine, respectively [24 (link)]. Bloodstream-form trypanosomes were propagated in vitro as described in [20 (link)] and adapted from [25 (link)]. Cells were counted with the CASY® Cell Counter system (Roche). Large numbers of trypanosomes for DNA isolation were obtained by inoculating female Naval Medical Research Institute (NMRI) mice (Harlan Laboratories) with 106 trypanosomes. At peak parasitemia, the trypanosomes were harvested and separated from the blood cells on DEAE-cellulose columns [26 (link)].
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