Tri sodium citrate dihydrate

Chitosan (CS, MW: 50–190 kDa, low molecular weight, 75–85% deacetylated), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), cinnamaldehyde, and sodium borohydride were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Mercaptosuccinic acid (MSA) and N-hydroxysuccinimide (NHS) were purchased from Alfa Aesar (Heysham, Lancashire, UK). 2-(N-morpholino) ethanesulfonic acid (MES) was obtained from ACROS Organics (Antwerp, Belgium). Silver nitrate was purchased from BDH Laboratory Suppliers (U) Ltd. (Kampala, Uganda). Tri-Sodium citrate dihydrate (TSC) was purchased from Nacalai Tesque (Kyoto, Japan).

Dulbecco’s PBS(−), heart-infusion broth, and trypticase soy broth were purchased from Nissui Pharmaceutical (Tokyo, Japan). HBSS supplemented with Ca²⁺ and Mg²⁺ was purchased from Gibco Invitrogen (Life Technologies, Carlsbad, CA, USA). Gentamycin sulfate, trisodium citrate dihydrate, dextran (MW ∼200,000 Da), paraformaldehyde, glutaraldehyde, OsO₄, and RIPA buffer containing a protease inhibitor cocktail were purchased from Nacalai Tesque (Kyoto, Japan). Cytochrome c, apocynin, DPI, sheep serum, and zymosan were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bafilomycin A1, glycine, SDS, 2-amino-2-hydroxymethyl-1,3-propanediol [Tris (hydroxymethyl) aminomethane], SOD, and polyoxyethylene [10 (link)] octylphenyl ether (Triton X-100) were purchased from Wako Pure Chemical Industries (Osaka, Japan). DNase was purchased from Roche (Basel, Switzerland).