Log In Sign up
The largest database of trusted experimental protocols

Anti phospho stat1 y701

Manufactured by Abcam
Visit Supplier

Anti-phospho-STAT1 (Y701) is a lab equipment product that detects phosphorylation of the STAT1 protein at tyrosine 701. This product can be used to monitor the activation status of the STAT1 signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Anti irf9, by Proteintech (1 mentions) Anti stat3, by Thermo Fisher Scientific (1 mentions) Anti α tubulin, by Abcam (1 mentions) Anti myc, by Roche (1 mentions) Recombinant human ifn α 2a, by Roche (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Recombinant ifn γ, by R&D Systems (1 mentions) Anti stat1, by Abcam (1 mentions) Anti mhcii, by Abcam (1 mentions)

Close spelling variants of this product

STAT1 (26 citations) Anti-STAT1 (16 citations) Anti-phospho-STAT1 (5 citations) Phospho-STAT1 (3 citations)

2 protocols using anti phospho stat1 y701

1

Antibody and Cytokine Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies and cytokines used were as follows: anti-α-tubulin (Epitomics), anti-phospho-STAT1 (Y701) (Abcam), anti-STAT1 (made in-house), anti-phospho-STAT2 (Y689) (Millipore), anti-STAT2 (made in-house), anti-phospho-STAT3 (Y705) (Epitomics), anti-STAT3 (Invitrogen), anti-IRF9 (Proteintech), anti-PLSCR2 (made in-house), anti-HA for immunoprecipitation and staining (made in-house), anti-HA for western blotting (clone: 12CA5), anti-myc (clone 9E10), recombinant human IFN-α2a (Roche), and recombinant murine IFN-α4 (made in-house).
Tsai M.H, & Lee C.K. (2018). STAT3 Cooperates With Phospholipid Scramblase 2 to Suppress Type I Interferon Response. Frontiers in Immunology, 9, 1886.
+ Open protocol
+ Expand
2

Regulation of Adipocyte MHCII Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was isolated from human subcutaneous differentiated adipocytes (Zen-Bio) using RIPA lysis buffer, treated with recombinant IFNγ (R & D), IFNγ + JAK inhibitor (Sigma), or IFNγ + STAT1 siRNA (Dharmacon) and IFNγ + scrambled RNA and subjected to Western blotting performed as previously described51 (link), using primary antibodies from Cell Signaling Technology: anti-phospho-STAT1 (Y701) (1:1000, 9171), anti-STAT1 (1:1000, 9172), and anti- MHCII (1:3000, 68258), from Abcam: anti-HLA-DPB1 (1:5000, ab157210), and from Santa Cruz: anti-β-actin (1:5000, sc-47778).
Bradley D., Smith A.J., Blaszczak A., Shantaram D., Bergin S.M., Jalilvand A., Wright V., Wyne K.L., Dewal R.S., Baer L.A., Wright K.R., Stanford K.I., Needleman B., Brethauer S., Noria S., Renton D., Joseph J.J., Lovett-Racke A., Liu J, & Hsueh W.A. (2022). Interferon gamma mediates the reduction of adipose tissue regulatory T cells in human obesity. Nature Communications, 13, 5606.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!