Tfap2c

Manufactured by Abcam

Sourced in United Kingdom, United States

TFAP2C is a transcription factor that belongs to the AP-2 family. It plays a role in the regulation of gene expression during development and cellular differentiation.

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Lab products found in correlation

Ez chip kit, by Merck Group (1 mentions) Enhanced chemiluminiscence solution, by Thermo Fisher Scientific (1 mentions) C caspase 3, by Abcam (1 mentions) β actin, by Abcam (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Bcl 2, by Abcam (1 mentions) Bcl2 associated x (bax), by Abcam (1 mentions) Phlpp1, by Proteintech (1 mentions) Trim29, by Proteintech (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) Gata2, by Abcam (1 mentions) Phospho smad1 5, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Klf17, by Atlas Antibodies (1 mentions)

Close spelling variants of this product

Rabbit-anti-TFAP2C

4 protocols using tfap2c

ChIP Assay for ZEB1 and TFAP2C

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Cells were harvested for chromatin immunoprecipitation (ChIP) by using an EZ-ChIP kit based on the manufacturer’s instructions (Millipore, Burlington, MA, USA). ZEB1 and TFAP2C were obtained from Abcam. The ChIP primer sequences are listed in Table S1. Immunoprecipitated DNA was quantified using qPCR. Purified DNA was quantified using real-time qPCR.

Chen J., Liu X., Xu Y., Zhang K., Huang J., Pan B., Chen D., Cui S., Song H., Wang R., Chu X., Zhu X, & Chen L. (2019). TFAP2C-Activated MALAT1 Modulates the Chemoresistance of Docetaxel-Resistant Lung Adenocarcinoma Cells. Molecular Therapy. Nucleic Acids, 14, 567-582.

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Western Blot Analysis of Cell Signaling Proteins

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Cold lysis buffer containing 50 mmol/l Tris–HCl (pH 7.4) was used to lyse the obtained cells. The DC protein assay kit (Bio-Rad Laboratories) was used to test the protein content, and a western blot was conducted according to the scheme of Hnasko's protocol. [23 ] Antibodies TFAP2C, TP53, P21, EGFR, Cylin D1, Bcl2, Bax, cCaspase-3 and β-actin were all bought from Abcam (Cambridge, UK). The enhanced chemiluminiscence solution (Life Sciences; Thermo Fisher Scientific) was used to visualize proteins.

Shi S., Huang X., Ma X., Zhu X, & Zhang Q. (2020). Research of the mechanism on miRNA193 in exosomes promotes cisplatin resistance in esophageal cancer cells. PLoS ONE, 15(5), e0225290.

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IHC Analysis of Subcutaneous Tumor Samples

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The collected subcutaneous tumors were fixed with 4% formalin, embedded in paraffin and then sectioned into 4-μm-slices. The standard protocol for IHC of the TMAs and subcutaneous tumors using a streptavidin-peroxidase (SP) Kit (Zhongshan biotech, Beijing, China), as described previously [25 (link)]. The slides were incubated with antibodies specific for TFAP2C (Abcam, cambridge, MA, USA), TRIM29 (Proteintech, Chicago, IL, USA), PHLPP1 (Proteintech, Chicago, IL, USA), p-AKT (Cell Signaling Technology, Danvers, MA, USA) and Ki-67 (Cell Signaling Technology, Danvers, MA, USA). The images of IHC staining were obtained by an Olympus microscope (Tokyo, Japan).

Jiang T., Wang H., Liu L., Song H., Zhang Y., Wang J., Liu L., Xu T., Fan R., Xu Y., Wang S., Shi L., Zheng L., Wang R, & Song J. (2021). CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer. Molecular Cancer, 20, 167.

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Pluripotency Marker Expression Analysis

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KLF17 (1:100; Atlas Antibodies, HPA024629), SOX2 (1:100; R&D systems, MAB2018), TFAP2C (1:500, Abcam, ab218107), OCT4 (1:200; Santa Cruz, sc5279), TBXT (1:500; Abcam, ab209665), GATA2 (1:500; Abcam, ab173817), KLF4 (1:200; Santa Cruz, sc20691), NANOG (1:400, Cell Signalling, 4893), PAR6 (1:200, Santa Cruz, sc67393), aPKC (1:200, Santa Cruz, sc216), β-catenin (1:300, Cell signalling, cs8480), ZO-1 (1:300, Thermo Fisher, ZO1-1A12), OTX2 (1:300, R&D systems, AF1979), CDX2 (1:100, Cell Signalling, 3977), Phospho-SMAD1/5 (1:100, Cell Signalling, 9516), LEF1 (1:100, Cell Signalling, 2230), ISL1 (1:50, Developmental Studies Hybridoma, 39.4DS), TFAP2A (1:100, Invitrogen, MA1-872), VTCN1 (1:250, Abcam, ab209242).

Bergmann S., Penfold C.A., Slatery E., Siriwardena D., Drummer C., Clark S., Strawbridge S.E., Kishimoto K., Vickers A., Tewary M., Kohler T.N., Hollfelder F., Reik W., Sasaki E., Behr R, & Boroviak T.E. (2022). Spatial profiling of early primate gastrulation in utero. Nature, 609(7925), 136-143.

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